AUTHOR=Xiao Fangyu , Qiu Jihua , Zhao Ying 

TITLE=Exploring the Potential Toxicological Mechanisms of Vine Tea on the Liver Based on Network Toxicology and Transcriptomics

JOURNAL=Frontiers in Pharmacology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2022.855926

DOI=10.3389/fphar.2022.855926

ISSN=1663-9812

ABSTRACT=Objective This paper focuses on whether vine tea contains potentially toxic components that trigger hepatotoxicity as a mechanism of action, which further provides some reference for the consumption and guide future product development of vine tea. Methods The chemical components of vine tea was collected from the reported literature and the toxicological information matched with CTD database was collected, and the data set of potential toxic components was established. The toxic components were submitted to the PharmMapper server to obtain potential targets. At the same time, the relevant targets were searched in CTD database and GeneCards database with keywords such as "Hepatic Toxicity", "Liver Damage" and "Drug-induced liver injury". After intersection, the potential hepatotoxic targets of vine tea were obtained. The protein interactions of potential hepatotoxic targets of vine tea were analyzed by STRING database. Protein-protein interaction (PPI) network were constructed by Cytoscape3.6.1 software. The GO molecular function and KEGG pathway of hepatotoxic targets were enriched by R package to screen the key targets. The role of components and key targets were analyzed by LEDOCK program . The data from GEO database was mined for the functional correlation characterized by cell transcriptional expression caused by vine tea as a disturbance factor. Results This study has searched 34 potential toxic components and 57 potential hepatotoxic targets of vine tea and the result showed that these targets were mainly involved in oxidative stress, cell metabolism and apoptosis to affect the liver. Conclusion Vine tea has the interrelationship of multi-components, multi-targets and multi-pathways. At the cellular level, the toxic components of Vine tea, mainly flavonoids, may promote oxidative stress, promote oxidation to produce free radicals, guide apoptosis, affect cell metabolism and other cytotoxic mechanisms. But this hepatotoxicity is related to the dose, duration of vine tea and individual differences. This study reveals the potential hepatotoxic components of vine tea, and provides a reference for further research and development of related functional products.