AUTHOR=Sang Xia’nan , Ying Jialiang , Wan Xuedong , Han Xin , Shan Qiyuan , Lyu Qiang , Yang Qiao , Wang Kuilong , Hao Min , Liu Erlong , Cao Gang 

TITLE=Screening of Bioactive Fraction of Radix Paeoniae Alba and Enhancing Anti-Allergic Asthma by Stir-Frying Through Regulating PI3K/AKT Signaling Pathway

JOURNAL=Frontiers in Pharmacology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2022.863403

DOI=10.3389/fphar.2022.863403

ISSN=1663-9812

ABSTRACT=Allergic asthma is a common respiratory inflammation disease. The crude Radix Paeoniae Alba (RPA) and its processed products have been used frequently as antipyretic and anti-inflammatory agents in traditional medicine. To evaluate the effect of honey and bran-processing, the different fractions were isolated from RPA for anti-allergic asthma through ovalbumin (OVA)-induced mice model, and then, the most effective fraction from RPA and Stir-frying Radix Paeoniae Alba with honey and bran (FRPA) for anti-allergic asthma were compared mutually on pharmacological effect. The results showed that the treatment of dichloromethane fraction from RPA significantly improved the pathological condition of lung tissue, decreased the number of eosinophils and other cells in bronchoalveolar lavage fluid (BALF), and the expression of various inflammatory factors. Further, the study discovered that the lung pathological conditions, compared with RPA high dose of dichloromethane fraction, could be ameliorated by FRPA high dose of dichloromethane fraction treatment. Moreover, the expression of inflammatory factors and the phosphorylation of the PI3K/AKT signaling pathway could be diminished by FRPA. Finally, the contents of compounds with the significant difference in FRPA dichloromethane fraction were paeoniflorin, ethyl gallate, pentagalloylglucose, galloylpaeoniflorin, and others by UPLC/Q-TOF-MS analysis. These findings suggest that the dichloromethane fraction of FRPA has an enhancement effect on anti-allergic asthma, and provide the experimental basis for exploring the processed mechanism of RPA.