AUTHOR=Noman Muhammad , Qazi Neelum Gul , Rehman Najeeb Ur , Khan Arif-ullah 

TITLE=Pharmacological investigation of brucine anti-ulcer potential

JOURNAL=Frontiers in Pharmacology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2022.886433

DOI=10.3389/fphar.2022.886433

ISSN=1663-9812

ABSTRACT=Gastric ulcer is one of the most common chronic gastrointestinal disease characterized by a significant defect in the mucosal barrier. The current study has been conducted to evaluate brucine anti-ulcer effect. Brucine has binding energy values of - 2.99 to - 8.11 Kcal/mol against chosen targets, according to in-silico research. Brucine exhibits inhibitory effect against helicobactor pylori. In-vivo findings revealed that brucine (3 mg/Kg) showed effective result in healing ethanol-induced ulcer lesions of gastric region in rats. Brucine shown inhibitory effect against H+/K+-ATPase. Levels of glutathione, glutathione-s-transferase and catalase enhanced in the gastric rat tissue with the use of brucine while significant decrease in lipid peroxide level was seen. Histopathological evaluation showed improvement in cellular architecture and decrease in inflammatory indicators like cyclooxygenase, tumour necrosis factor, and nuclear factor kappa B expression, validated through immunohistochemistry, enzyme-linked immunosorbent assay and western blot techniques. In the reverse transcription-polymerase chain reaction brucine decreased H+/K+-ATPase mRNA levels. This study reveals that brucine possess stable binding affinities against selected targets. Brucine exhibits anti-ulcer effect, mediated via anti-H. pylori, H+/K+-ATPase inhibition, anti-oxidant and anti-inflammatory pathways.