AUTHOR=Araya Nicolas , Leiva-Soto Martín A. , Bruna Maria V. , Castro-Munoz Almendra , Behrend-Keim Beatriz , Moraga-Espinoza Daniel , Bahamondez-Canas Tania F. 

TITLE=Formulation of water-soluble Buddleja globosa Hope extracts and characterization of their antimicrobial properties against Pseudomonas aeruginosa

JOURNAL=Frontiers in Pharmacology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2022.921511

DOI=10.3389/fphar.2022.921511

ISSN=1663-9812

ABSTRACT=Buddleja globosa Hope (BG) extracts are traditionally used to treat skin and gastric ulcers due to their healing properties. Non-aqueous solvents such as ethanol and DMSO are usually used to extract naturally occurring compounds. However, the cytotoxicity of these solvents and the low water-solubility of the extracted compounds can hinder their biomedical applications. To overcome the limited solubility of the BG extracts, we aimed to enhance the solubility by processing a standardized hydroalcoholic extract (BG-126) through spray drying (SD), with and without two solubility enhancers. Spray dried BG extract (BG-SD) and spray dried BG extracts plus polyvinylpyrrolidone (BG-SD PVP) and Soluplus® (BG-SD SP) were developed starting from BG-126 (containing 53% ethanol). These four formulations were characterized by total phenolic content, water solubility at 25 and 37 °C, and antimicrobial properties against Pseudomonas aeruginosa. All the SD formulations presented a solubility that allowed to reach maximum concentrations of 1024 µg/mL catechin for BG-SD and 2048 µg/mL catechin for BG-SD PVP and BG-SD SP for antimicrobial testing. BG-SD showed the highest antimicrobial potency with a minimum inhibitory concentration (MIC) of 512 µg/mL catechin, followed by BG-126 with a MIC of 1024 µg/mL catechin and SP. BG-126 also showed to inhibit biofilm formation as well as the excipients PVP and SP. Spray dried BG extract (BG-SD) represents a promising natural active with enhanced antimicrobial properties against P. aeruginosa for further research and development of novel phytopharmaceuticals.