AUTHOR=Liu Guanjie , Chang Ling , Qian Yihan , Lin Jiacheng , Shang Zhi , Xu Min , Wang Fang , Sun Xuehua , Jiang Yun , Gao Yueqiu , Kong Xiaoni 

TITLE=Quantitative proteomics reveals Polygonum perfoliatum L. ameliorates hepatic steatosis by promoting PPARs/CPT1A/CPT2-mediated fatty acid β-oxidation

JOURNAL=Frontiers in Pharmacology

VOLUME=Volume 14 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2023.1016129

DOI=10.3389/fphar.2023.1016129

ISSN=1663-9812

ABSTRACT=Non-alcoholic fatty liver disease (NAFLD) is a prevailing cause of end-stage liver disease in the coming decades. Polygonum perfoliatum L. (PPL) is a medicinal herb with anti-lipid peroxidation and anti-inflammatory properties, exerting a hepatoprotective effect. However, the protective effect of PPL against NAFLD and its underlying mechanism remain incompletely understood. Here, we show that PPL protects high fat diet (HFD)-induced hepatic steatosis, lipid peroxidation, and glucose-lipid metabolism dysfunction in NAFLD mice. We performed a label-free quantitative proteomic profiling to determine the effect of PPL treated the proteomics profile of liver tissue and identified activated PPARs/CPT1A/CPT2-mediated hepatic fatty acid β oxidation (FAO) process was significantly altered. In vitro treatment of hepatocytes with PPL extract confirmed these altered process and FAO inhibitor Etomoxir (ETO) attenuated the lipid-lowering activity of PPL extract in hepatocytes. Ultra-high-performance liquid chromatography/ Q Exactive-HFX (UPLC/QE-HFX) was used to determine the material basis for PPL against NAFLD. Our results demonstrated the effects and potential mechanisms of PPL as an effective pharmacological treatment of NAFLD.