AUTHOR=Jiang Xue-Lian , Ma Gai-Fan , Zhao Bin-Bin , Meng Yan , Chen Lin-Lin 

TITLE=Structural characterization and immunomodulatory activity of a novel polysaccharide from Panax notoginseng

JOURNAL=Frontiers in Pharmacology

VOLUME=Volume 14 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2023.1190233

DOI=10.3389/fphar.2023.1190233

ISSN=1663-9812

ABSTRACT=A water-soluble polysaccharide (PNPB1) was isolated from rhizome of Panax notoginseng by anion exchange and gel permeation chromatography. The molecular weight of PNPB1 was determined to be 9.3×105 Da. Monosaccharide composition analysis indicated that PNPB1 consisted of Glc (88.2%), Gal (9.0%), Ara (2.4%) and trace GlcA, with the major backbone of (1→4)-linked α-Glcp, (1→6)-linked β-Glcp, and (1, 4→6)-linked β-Glcp. The immunoregulatory function of PNPB1 was further investigated in cyclophosphamide induced immunosuppressive mice and immune cells. The polysaccharide was found to significantly enhance murine body weight, improve their thymus and spleen indices and increase the white blood cells (WBC). PNPB1 significantly enhanced splenic lymphocyte proliferation, NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production, as well as the phagocytosis and TLR2 expression of peritoneal macrophages, indicating potent immunoenhancement effect. These findings provide a theoretical basis for elucidating the structure and immune activity of notoginseng polysaccharides.