The traditional uses, pharmacology, and phytochemistry of Peucedanum praeruptorum Dunn

Bai Hua Qian Hu (Qianhu; Peucedanum praeruptorum Dunn) is a classical medicinal plant traditionally prescribed for respiratory ailments, including cough, pulmonary hypertension, and asthma. In this review, we summarize the research progress of the toxicology, pharmacokinetics, pharmacology, phytochemistry, botany, quality control, and traditional uses of P. praeruptorum in order to support future investigations into the scientific and therapeutic promise of this important medicinal plant. Information pertaining to P. praeruptorum was collected from scientific databases (ScienceDirect, Springer, SciFinder, PubMed, Baidu Scholar, Google Scholar, Web of Science), as well as toxicology papers from local conferences, M. Sc. and Ph.D. theses and dissertations, local magazines, classic texts on Chinese botanical drugs, and peer-reviewed journals. The Plant List (www.theplantlist.org) was utilized to verify the taxonomy of P. praeruptorum. P. praeruptorum was found to contain more than 119 distinct phytochemicals, including simple coumarins, pyranocoumarins, furanocoumarins, flavonoids, ketones, organic acids, and sterols, among others (e.g., praeruptorins A and B). Both crude plant extracts and purified metabolites of P. praeruptorum have been reported as treatments for hypertension, osteoporosis, Huntington’s disease, and cancer. In addition, extracts of P. praeruptorum are reported to exhibit diverse pharmacological activities, including osteogenic, anti-osteoclastogenic, antidepressant, neuroprotective, antitumor, and anti-inflammatory effects. Research into the pharmacology and phytochemistry of P. praeruptorum partially support both traditional uses and extraction methods. However, further research is required to elucidate the relationships between these metabolites, their molecular mechanisms, their structure-function roles, and their antagonistic and synergistic effects.


GRAPHICAL ABSTRACT 1 Introduction
Peucedanum L. (Umbelliferae) consists of 120 species of herbaceous perennial plants which are distributed widely across the globe (Editorial at an altitude of 250-2000 m (Liu et al., 2021).P. praeruptorum can reach a height of 60-100 cm.The plant has a cylindrical stem, with a glabrous lower part and piliferous branches on the upper part.The medulla is solid.Leaves are wide ovate or triangular ovate.The compound umbel is terminal or lateral, and 3.5-9 cm in diameter.The fruits are oval, 4 mm in length and 3 mm in width.The back of the fruit is flat.The rhizomes are strong, brown, and 1-1.5 cm in diameter.The roots are conical and branched, with thin root ends.
For medicinal use, roots are collected from winter to spring.Abluent, fresh, thin slices are dried prior to medicinal use.

Traditional uses
The List of Mingyi Bielu (I名医别录J), which dates to the Wei-Jin and South-North Dynasties (A.D. 220-450), was the first to The aerial tissues (A), medicinal root tissues (B), and commercial presentation (C) of Peucedanum praeruptorum Dunn.

FIGURE 2
The natural distribution of Peucedanum praeruptorum Dunn across southern China.

Anti-inflammatory activity
Praeruptorin C (pyranocoumarins) was traditionally used as an antibechic and antibronchitic drug.In one study, praeruptorin C treatment was found to regulate excitatory synaptic proteins in the anterior cingulate cortex, attenuate the release of proinflammatory cytokines, and inhibit the activation of microglia (Su et al., 2021).However, further studies need to assess the effects of praeruptorin C in other pain models.In another study, administration of (±)-praeruptorin A (30, 60, 120 mg/kg) to ovalbumin-sensitized BALB/c mice for 56 days increased the level of INF-γ and reduced the expression of IL-13/-4 in bronchoalveolar lavage fluid (BALF); decreased the level of immunoglobulin (Ig) E in serum; and suppressed airway inflammation, hyperresponsiveness, and remodeling.In the study, levels of cytokines in BALF, immunoglobulin (Ig) E in serum as well as expression of TGF-β1 and Smad proteins in lung tissue were measured by enzyme-linked immunosorbent assay, immunohistochemistry or Western blot analysis.(Xiong et al., 2012).Treatment of inflamed human periodontal membrane cells with praeruptorin D (10, 20, 30, 40 μg/mL) inhibited TNF-α and IL-1β expression.According to experimental requirements, the negative control group was healthy cells group and positive control group was minocycline hydrochloride group (Yu, 2022).Administration of praeruptorins C, D, and E (2, 4, 8, 16 μg/mL) to RAW264.7 macrophages stimulated with lipopolysaccharide (LPS) for 24 h inhibited NF-κB and STAT3 activation.Although all three had anti-inflammatory activities, praeruptorins D and E exhibited greater anti-inflammatory activities than praeruptorin C and in vivo pharmacological potencies need to be further evaluated.(Yu et al., 2012).One study demonstrated that the administration of IMP (furanocoumarins) at doses of 5, 10, and 15 μmol/L for 1 h in the RBL-2H3 allergic inflammatory cell model promoted IFN-γ expression; decreased the expression of TNF-α, COX-2, IL-6, IL-4, and IL-3; and inhibited RBL-2H3 cell degranulation.These results indicate that IMP is effective for inhibiting the inflammatory response in the RBL-2H3 allergic inflammatory cell model mediated by IgE immunoregulation (Long et al., 2019).However, the authors did not evaluate the effectiveness of IMP using animal models.Moreover, P. praeruptorum polysaccharide treatments ranging from 25 to 200 μg/mL increased the expression of costimulatory and accessory factors, increased the secretion of chemokines and inflammatory factors, and enhanced phagocytosis and pinocytosis.In this way, P. praeruptorum polysaccharides modulated the inflammatory response of macrophages via the NF-κB and TLR2/TLR4-dependent MAPK pathways (Zhao et al., 2022).Another study demonstrated that Dl-praeruptorin A reduced inflammation in an LPS-induced acute lung injury mouse model, specifically inhibiting endothelial inflammation (Wang et al., 2012;Zhou et al., 2016).Nonetheless, the    "-" denotes no useful information found in the study.
Frontiers in Pharmacology frontiersin.orgmechanism of Dl-praeruptorin A against acute lung injury needs further study prior to its use in clinical treatment.

Expectorant and antitussive effects
P. praeruptorum water extract treatment (45 g/kg) was found to resolve phlegm (Liu et al., 1997;Meng et al., 1997).The administration of praeruptorin C and nodakenin (10 mg/kg) to mice, with phenolsulfonphthalein as an expectorant indicator, increased phenolsulfonphthalein excretion in tracheal tissues.Both praeruptorin C and nodakenin showed expectorant effects.And ammonium chloride served as a positive control (Liu et al., 2009).However, phenolsulfonphthalein is mainly excreted from the urine by the kidneys, and drugs that affect renal function are prone to false positive results.Similarly, treating BALB/c mice for 5 days with nodakenin (10 mg/kg) promoted the DNA binding activity of NF-κB, increased the levels of IκBα and P65 in the cytoplasm, decreased the expression of p-P65 and P65 in the nucleus, reduced the level of IgE in serum and IL-13/-5/-4 in BALF, and suppressed airway hyperreactivity and inflammation (Xiong et al., 2014).Notably, the pharmacological properties of P. praeruptorum before and after honey roasting were found to be different.The 5.0 and 10.0 g/kg doses of honey-roasted products showed stronger expectorant and antitussive effects than raw products.However, the 2.5 g/kg dose of honey-roasted and raw products was more effective as relieving asthma (Zhang et al., 2010b).Although P. praeruptorum has long been used to resolve phlegm, descend Qi, clear heat, and dissipate wind in TCM (Zhao et al., 2022), comprehensive studies of its constitutive bioactive monomers and their molecular mechanisms, as well as clinical trials, are necessary to improve its expectorant and antitussive activities with minimal side effects.

Antitumor activity
More than 85% of all kidney cancers worldwide are characterized as renal cell carcinoma (RCC).In one study, treating RCC cells for 24 h with praeruptorin B (0-30 μmol/L) inhibited both migrability and invasibility, as well as downregulated the expression of cathepsins V and C in ACHN and 786-O cells (Lin et al., 2020).Similarly, praeruptorin B (20, 40, 60 μmol/L) was reported to inhibit both the proliferation and migration of SK-OV-3 ovarian cancer cells, as well as downregulate the expression of FASN, c-Myc, SREBP-1c, and cyclin D1.The likely mechanism is that the SREBP-1c/FASN signaling pathway regulates the energy metabolism of SK-OV-3 ovarian cancer cells, thus inhibiting their proliferation.Realtime fluorescence quantitative polymerase chain reaction (RT-qPCR) was used to detect the mRNA expressions of proliferating genes such as c-Myc and CyclinD1 and the mRNA expressions of key genes of energy metabolism such as SREBP-1c and FASN in tumor cells and Western blot was used to detect the expressions of SREBP-1c, FASN proteins (Xue et al., 2021).
A 48 h treatment with praeruptorin A (25 μmol/L) combined with taxol (125 nmol/L) induced apoptosis in A2780/TAX ovarian cancer cells, and inhibited their migration by downregulating MMP9 and MMP2 expression.However, it remains to be seen whether praeruptorin A can enhance the chemotherapeutic efficacy of taxol, or whether it retains its curative effect against ovarian cancer in vivo (Chen et al., 2022).Another study on HeLa and SiHa cell lines reported that praeruptorin A could increase the levels of tissue inhibitors of metalloproteinase-2 and decrease the expression of matrix metalloproteinase-2; downregulate S-phase kinaseassociated protein 2 and cyclin D1; upregulate p27, p21, p16, and Rb; and induce G 0 /G 1 phase cell cycle arrest.However, praeruptorin A could not inhibit cell viability in IgG-treated cells, the effect of IgG interference with praeruptorin A in HeLa cells (Wu et al., 2017).Additionally, praeruptorin A has been found effective at inhibiting the migrability and invasibility of hepatocellular carcinoma (HCC) cells by activating extracellular signal-regulated kinase signaling and inhibiting matrix metalloproteinase-1 expression.However, in vivo metastasis animal model is even worthier for further investigation to examine the antimetastatic effect and safety evaluation of praeruptorin A (Yu et al., 2021).In LS174T cells, praeruptorin A (2.5, 10, 40 μmol/L) could significantly upregulate cytochrome P450 3A4 levels and activity via a pregnane X receptor-mediated pathway.However, siRNA knockdown of the pregnane X receptor resulted in suppressed expression of cytochrome P450 3a11 in mouse primary hepatocytes (Huang et al., 2013).
Administration of praeruptorins A and B to SGC7901 human gastric cancer cells for 24 h produced cytotoxic effects in SGC7901 cells, resulting in antiproliferation.praeruptorin A could also enhance the action of doxorubicin on SGC7901 cells (Liang et al., 2010).Administration of praeruptorins A and C (10, 25, 50 μmol/L) to HepG2 cells in vitro for 24 or 48 h increased the expression of multidrug resistance-associated protein 2 by way of the constitutive androstane receptor-mediated pathway (Zhou et al., 2013).In H1299, PC9, H1975, and PC9/ER human non-small-cell lung cancer (NSCLC) cell lines, praeruptorin A and pteryxin restricted the HGF-induced phosphorylation of MET in PC9/ER and H1975 cells, increased PARP cleavage in H1975 cells and the proportion of annexin V-positive cells, and overall induced apoptosis and reduced cell viability.However, praeruptorin A and pteryxin could not inhibit HGF-induced AKT phosphorylation and prompted apoptosis in NSCLC cells regardless of EGFR TKI resistance or epidermal growth factor receptor (EGFR) mutation status (Park et al., 2022).

Neuroprotective activity
One study demonstrated that 24 h of treatment with praeruptorin C (0, 1, and 10 μmol/L) could partially reverse the upregulated expression of GluN2B-containing N-methyl-Daspartate receptors, inhibit neuronal apoptosis, and balance Bax and Bcl-2 expression.Although it was suggested that praeruptorin C exerted its neuroprotective effects by reversing intracellular Ca 2+ overload, it is possible other pathways or mechanisms were involved (Yang et al., 2013).In another study, praeruptorin C (1.5, 3.0 mg/kg) was found to alleviate depressive behavior, motor deficit, and neuronal excitotoxicity in 3-nitropropionic acid (3-NP)-treated mice via upregulating the expression of HTT, DARPP32, and BDNF in striatum tissue.Motor behavior was tested using the open field test and rotarod test, while psychiatric symptoms were tested using the forced swimming test and tail suspension test.We suggest that, based on these findings, praeruptorin C may prove therapeutic for cognitive, psychiatric, and movement disorders associated with Huntington's disease (Wang et al., 2017).

Anti-osteoclastogenic activity
Osteoporosis results in an elevated risk of fracture, compromised bone strength due to low bone density, and metabolic defects.Bone homeostasis depends on the resorption of bone by osteoclasts and formation of bone by osteoblasts.Imbalance of this tightly coupled process can cause diseases such as osteoporosis (Chen et al., 2018).In one study, exposure of RAW264.7 cells for 4 h to praeruptorin C (0, 20 μmol/L) reduced osteoclast formation by obstructing the JNK and NF-κB pathways, without disturbing the p38 and ERK pathways.In ovariectomized (OVX) mice, a model for post-menopausal bone loss, praeruptorin C was found to increase bone mass and decrease osteoclast activity.The antiresorptive properties of praeruptorin C suggest that it may be an effective treatment for osteoporosis, although further research is required (Liu et al., 2017).In bone marrow-derived macrophages, praeruptorin A (10 μmol/L) treatment for 30 min inhibited RANKL-stimulated osteoclast differentiation and p38 and Akt signaling (Yeon, Jeong-Tae, et al., 2014).Praeruptorin D could promote the osteogenic differentiation and proliferation of inflammatory periodontal membrane cells, and upregulate osteogenic gene (RUNX-2, ALP, and OCN) expression at doses of 10, 20, 30, and 40 μg/mL by using RT-qPCR and Alizarin red S staining (Yu, 2022).

Antidepressant activity
Clinical depression is characterized by sustained depressive mood and cognitive dysfunction, including restlessness, anhedonia, sleep disorders, guilt, and repeated thoughts of suicide (Liu et al., 2011).The occurrence of depression is closely related to damage to hippocampal neurons.Chronic stress can damage the hippocampus, resulting in atrophy, apoptosis, or reduced regeneration of hippocampal neurons (Wang and Xu, 2014).Dl-praeruptorin A can protect the nervous and cardiocerebrovascular systems.For example, 28 days of treatment with Dl-praeruptorin A (10, 30, 60 mg/kg) improved the synaptic ultrastructure of hippocampal CA 1 region and increased neurotrophic factors and nerve growth factors in the hippocampus (Wang and Xu, 2014).In addition, 28 days of treatment with IMP (15, 30 mg/kg) significantly enhanced 5-HT 1A R expression, 5-HT level, and sucrose preference; increased the incidence of grooming, rearing, and crossing behaviors; reduced immobility; and decreased 5-HTT expression.These results indicate that IMP exhibits antidepressant effects in rats, likely due to changes in the concentration of 5-HT and 5-HTT, and in the expression of 5-HT 1A R, in the hippocampus and prefrontal cortex (Zheng et al., 2019).However, the mechanism responsible for the antidepressant activity of P. praeruptorum extracts remains unknown, and clinical pharmacological experiments are lacking.

Other activities
P. praeruptorum has been shown to exhibit other therapeutic activities, including ventricular remodeling, inhibiting hepatic microsomal drug-metabolizing enzymes, ameliorating memory disruption, and alleviating ischemia/reperfusion injury.Administration of P. praeruptorum alcohol extracts to rats for 28 days affected ventricular remodeling and apoptosis-related proteins in different ways, and had a positive influence on ventricular remodeling (Tu et al., 2004).Treatment of mice with total coumarins (50, 100, 200 mg/kg) prolonged the hypnotic duration of pentobarbital sodium in a dose-dependent manner, inhibited the activities of aniline hydroxylase and aminopyrine N-demethylase, minimally influenced the hypnotic effect of barbital sodium, and inhibited the activity of hepatic microsomal drug-metabolizing enzymes (Wang et al., 2004).In addition, administration of nodakenin (10 mg/kg) reduced scopolamineinduced cognitive impairments associated with the Y-maze test and passive avoidance test, as well as minimized escape latency in the Morris water maze test.Nodakenin has been shown to block acetylcholinesterase activity in a dose-dependent manner in vitro (Kim et al., 2007).Administration of Dl-praeruptorin A (0.5, 1.0, 2.0 mg/kg) reduced the levels of bcl-2, bax, Fas, and IL-6, and raised the bcl-2/bax ratio, under hypotension without bradycardia.A positive, linear correlation has been demonstrated between bax, Fas, bcl-2, and IL-6, where neutrophil infiltration was minimal.Dlpraeruptorin A was also found to prevent postischemic cell death in rat heart, likely due to the automodulation of immediate-early gene expression of bax, Fas, bcl-2, and IL-6 during myocardial ischemia/ reperfusion (Chang et al., 2003).In rats, praeruptorin C (5, 15, 30 mg/kg) was found to reduce ischemia/reperfusion injury induced by coronary ligation, most likely by reducing oxygen free radicals (Liu and Wang, 2009).

Pharmacokinetic studies
Praeruptorins A, B, and C, the primary metabolites of P. praeruptorum, exhibit diverse biological activities, including neuroprotective, antitumor, anti-inflammatory, immunoregulatory, anti-osteoclastogenic, and antidepressant effects.Recently, researchers developed a liquid chromatography-selected ion monitoring-mass spectrometry (LC-SIM-MS) method to conduct a pharmacokinetic study of WaiGan KeSou Formula decoction administered to rats at a dose of 20 mL.The monarch drug of WaiGan KeSou Formula was Qianhu, and its index compound was Praeruptorin A. Within 24 h, the peak concentration (C max ) of praeruptorin A in plasma was 172.697 ± 17.254 ng/mL, the peak time was 1.50 h, the elimination half-life (t 1/2 ) was 1.02 h, the mean retention time was 3.42 h, the AUC 0-τ (area under the curve) was 504.866 ± 50.317 h ng/mL, and the AUC 0-∞ was 514.401 ± 36.950 h ng/mL (He and Wu, 2021).In addition, LC-MS/MS was utilized to evaluate the plasma concentrations of praeruptorin A in rats after a single intragastric dose of 8 g/kg body weight.The researchers found that praeruptorin A was detectable up to 24 h after administration, with an AUC 0-t of 311.80 ± 42.38 ng h/mL, a C max of 31.09± 4.84 ng/mL, and a t 1/2 of 7.52 ± 1.00 h (Zhou et al., 2015).
A sensitive, selective, and rapid online solid phase extractionchiral LC-MS/MS method was developed to conduct a pharmacokinetic study of praeruptorins B and C after orally administering P. praeruptorum extract to rats.Praeruptorins B and C were detectable in rat plasma up to 24 h after administration, with AUC 0-t values of 187.29 ± 15.02 (B) and 91.64 ± 9.37 h ng/mL (C), C max values of 19.66 ± 4.25 (B) and 7.59 ± 1.98 ng/mL (C), and t 1/2 values of 8.20 ± 1.21 h (B) and 14.97 ± 3.66 h (C) (Zhou et al., 2015).Nonetheless, additional pharmacokinetic studies should be conducted on the other bioactive metabolites present in P. praeruptorum, including praeruptorin D, praeruptorin E, qianhucoumarin B, and praeroside I.

Quality control
Qianhu is typically processed by washing and immediately drying at low temperature, which must be kept below 60 °C.Fresh slices should be thicker than 6 mm (Ren et al., 2021).To maintain medicinal quality, the Chinese Pharmacopoeia dictates the use of microscopic, morphological, HPLC, and TLC detection and identification, as well as ethanol extraction and cold-dipping.By utilizing cold-dipping, the ethanol extract must be more than 20.0% for P. praeruptorum.According to the requirements of the Chinese Pharmacopoeia (Chinese Pharmacopoeia Committee of People's Repulic of China, 2020), the moisture content (after drying) must not exceed 12.0% and the ash content must not exceed 8.0%.Moreover, different extraction methods have different effects on the index metabolites of P. praeruptorum (praeruptorins A and B).The reflux method is favored for the extraction of praeruptorin A, producing a much higher praeruptorin A content than ultrasonic methods.Conversely, the ultrasonic method is favored for the extraction of praeruptorin B, producing a much higher praeruptorin B content than the reflux method (Xu et al., 2022).However, it is inadvisable to utilize only one crude, quantitative marker when assessing the quality of P. praeruptorum extracts.An array of bioactive metabolites has been detected in P. praeruptorum by HPLC, UV, gas chromatography (GC), NMR, high-speed counter-current chromatography coupled with electrospray ionization multi-stage MS (prep-HSCCC/ESI-MS(n)) (Zhou et al., 2015).
The medicinal quality of P. praeruptorum is affected by the altitude at which it is produced.According to reports by Luo et al. (2022), an altitude of 900 m improved the praeruptorin A content, while an altitude of 650 m improved the praeruptorin B content.Moreover, the influence of altitude was greater on praeruptorin B content than on praeruptorin A content (Luo et al., 2022).In another study, praeruptorin A and B contents were higher in plants cultivated at high altitudes than in plants cultivated at low altitudes by using HPLC-DAD method to determine the contents of praeruptorin A and B in the 24 batches of Qianhu from different producing areas.According to the experimental results, cluster analysis and principal component analysis were carried out (Yang et al., 2021).In addition, the key climatic factors affecting the praeruptorin content are average relative humidity, average maximum temperature in July, average annual temperature, and average temperature in July (Xu et al., 2021).However, the relationships between Qianhu quality and climatic factors have not been widely investigated, and warrant further study.
Praeruptorin A and B contents are also influenced by plant organ, harvesting time, cultivation environment, and fertilization strategy.According to reports by Li et al. (2022), the HPLC method was used to determine the content of praeruptorin A and B in the cultured P. praeruptorum at different harvesting periods, as well as to analyze the fluctuation of praeruptorin A and B at different harvesting periods.Praeruptorin A and B contents are highest in P. praeruptorum roots, followed by stems, and are lowest in leaves.In the 14 samples, the praeruptorin A content was found to be much higher in 2-year-old P. praeruptorum than in 1-year-old P. praeruptorum.Moreover, plants cultivated on southern slopes exhibited an approximately 80% higher content of praeruptorin A than plants grown on slopes of other orientations.However, no significant differences in the contents of praeruptorin A or B were observed in P. praeruptorum harvested before or after bolting (Li et al., 2022).Finally, the reasonable application of P and K fertilizers has been found to improve both the yield and medicinal quality P. praeruptorum, although the application of N fertilizer should be controlled (Zhou et al., 2022).

Safety
Praeruptorin C exerted no toxicity on primary cultures of mouse neurons at doses of 0 and 10 μg/mol (Yang et al., 2013).Moreover, an emulsion of praeruptorin C did not exert any toxicity or induce any behavioral changes at doses of 5 and 40 μg/mol in OVX mice by performing a CCK-8 assay (Liu et al., 2017).In BMMs, praeruptorin A was found to be atoxic at doses under 10 mmol/L, but significantly cytotoxic at doses over 20 mmol/L (Yeon et al., 2014).In HepG2 cells, praeruptorins A and C were found to be atoxic at doses of 10 and 100 μg/mol, respectively (Zhou et al., 2013).However, a maximum dose of 200 μg/mol dramatically increased cell toxicity.The toxicity of praeruptorin B on SK-OV-3 ovarian cancer cells was not obvious at doses of 20 and 60 μmol/L (Xue et al., 2021).HCC cell line and normal liver THLE-2 cells were treated with different concentrations of praeruptorin A (0, 10, 20, 30, 40 μg/ mL) for 24 h.The cell viability was assessed through 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay.The results implied that praeruptorin A did not induce cytotoxicity in an HCC cell line at doses of 10 and 40 μg/mL (Yu et al., 2021).In addition, praeruptorin A was atoxic to normal liver THLE-2 cells under at concentrations of 10-30 μg/mol.The Chinese Pharmacopoeia 2020 (Chinese Pharmacopoeia Committee of People's Repulic of China, 2020) recommends a Qianhu dosage of 3-10 g/d.In addition, Qianhu can safely be used with Pinelliae Rhizoma, but not with Gleditsia sinensis Lam. or Veratrum nigrum L. ("Bencaojing Jizhu" I本草经集注J).Finally, Qianhu is not considered suitable for people with Yin deficiency syndrome (a series of symptoms caused by the deficiency of yin essence or fluid in the human body.Yin: the dark, not active, female principle of the Universe in Chinese philosophy) (Wen, 2023), cough, cold, or cold fluid syndrome.According to cell studies, P. praeruptorum appears to be atoxic at a low dose but cytotoxic at higher doses (Zhou et al., 2013;Yeon et al., 2014).
10 Conclusion and future perspectives P. praeruptorum is a classical medicinal plant commonly used in TCM preparations.Here, we systematically evaluated the toxicology, molecular mechanisms, pharmacology, phytochemistry, botany, quality control, and traditional uses of P. praeruptorum in order to validate the medicinal use of this species.According to the Chinese Pharmacopoeia and classical Chinese botanical drugs, P. praeruptorum has been historically prescribed to treat a wide spectrum of diseases including cough, asthma, and pulmonary hypertension.Pharmacological studies suggest that P. praeruptorum exhibits anti-inflammatory, expectorant, antitussive, antitumor, neuroprotective, anti-osteoclastogenic, and antidepressant effects, and largely support the traditional uses of this plant.To date, more than 119 distinct phytochemicals have been identified in P. praeruptorum extracts, the most common of which are pyranocoumarins and furanocoumarins.
Although there has been considerable progress in evaluating the phytochemistry and pharmacology of P. praeruptorum, there are still gaps in our knowledge.First, according to the Chinese Pharmacopoeia 2020 (Chinese Pharmacopoeia Committee of People's Repulic of China, 2020), Qianhu can disperse wind-heat, reduce cough and phlegm, and dissipate adverse Qi, indicating that Qianhu may alleviate the effects of wind-heat on the lungs (Song et al., 2015).However, most of the active metabolites of P. praeruptorum effective against cough and wind-heat are currently administered as crude extracts.Therefore, comprehensive investigations should be conducted to identify the effective metabolites and elucidate their modes of action in order to facilitate clinical trials.Second, we found few reports on the toxicity of P. praeruptorum extracts or potential botanical drugs interactions.The potential adverse effects, contraindications, and toxicities of P. praeruptorum extracts and their bioactive metabolites should therefore be studied in vitro, in vivo, and in clinical trials.Third, the majority of the reviewed research was conducted in cell cultures or animal models.Clinical trials in humans will be required to truly evaluate the efficacy P. praeruptorum in addressing depression, osteoporosis, cancer, and inflammation, among other diseases.Forth, most of the P. praeruptorum containing health products are mainly derived from its root rich in chemical compounds, while non-medicinal parts are rarely exploited.Therefore, it may be interesting to extend the research to the non-medicinal parts of the inexpensive flowers, leaves, and stems of P. praeruptorum to ensure the fully utilization of its edible and medicinal values.Finally, new and updated analytical and quality control methods will be required to identify novel markers of quality for the assessment of TCM preparations.
In conclusion, P. praeruptorum is rich in medicinal materials, and its pharmacological effects are extensive.With the advantages of modern instruments and data analysis technology in identifying chemical components and separation, Qianhu medicinal materials can be better developed and new drug discovery (Liu, 2020).Future research should be conducted to investigate the mode of action responsible for the pharmacological activities of P. praeruptorum extracts, as well as to comprehensively evaluate the potential toxicities, adverse effects, and contraindications of this botanical drugs.Alongside updated quality control measures, these investigations will facilitate clinical trials.Updated in vivo pharmacological studies must be performed to validate the traditional uses of P. praeruptorum.Finally, the clinical safety and efficacy of P. praeruptorum-derived phytochemicals in the treatment of depression, osteoporosis, cancer, and other diseases, require validation.

FIGURE 1
FIGURE 1 from the Chinese Pharmacopoeia 2005 (Chinese Pharmacopoeia Committee of People's Repulic of China, 2005).More recently, the Chinese Pharmacopoeia 2010, 2015, and 2020 (Chinese Pharmacopoeia Committee of People's Repulic of China, 2010; Chinese Pharmacopoeia Committee of People's Repulic of China, 2015; Chinese Pharmacopoeia Committee of People's Repulic of China, 2020) define Qianhu as the roots of P. praeruptorum exclusively, while Zihuaqianhu is defined as the roots of P. decursivum.

FIGURE 8
FIGURE 8Possible mechanisms associated with the pharmacology of Peucedanum praeruptorum Dunn.
4H-1-benzopyran-4-one,5-hydroxy-6methoxy-2-phenyl-7-O-α-D-glucuronyl acid 1034H-1-benzopyran-4-one,5-hydroxy-6methoxy-2-phenyl-7-O-α-D-glucuronyl methyl ester 116 Root MS, NMR, OCC, SGCC Ethanol Zhang et al. (2012) Polyacetylene 117 Root CS, NMR, HR-ESI-MS Ethanol Lee et al. (2015) (Continued on following page) Frontiers in Pharmacology frontiersin.orgrespiratory failure, acute bronchitis, and iridocyclitis after cataract surgery (Wang, 2016).The roots of P. praeruptorum have been utilized in a variety of traditional preparations, and are often used in combination with Mentha haplocalyx Briq., Arctium lappa L., and Platycodon grandiflorum (Jacq.) A. DC. to treat external wind-heat, body heat, headache, cough, and phlegm.In addition, P. praeruptorum is used in combination with Citrus reticulata Blanco, Pinellia ternata (Thunb.)Breit., and Prunus armeniaca L. var.ansu Maxim.to treat cough, chest congestion, vomiting, and nausea.The roots of P. praeruptorum have been used in more than 189 TCM preparations and 525 classical prescriptions (https://db.yaozh.com,accessed 7th December, 2023).Examples of TCM prescriptions containing P. praeruptorum are listed in Table 1."Bai He Qian Hu Tang," "Da Qian Hu Tang," "Fu Ling Qian Hu Tang," "Jiu Wei Qian Hu Tang," and "Xing Su San" are Chinese classical prescriptions recorded in many ancient books.The botanical drugs "Tong Xuan Li Fei Ke Li" and "Tong Xuan Li Fei Pian" (Chinese Pharmacopoeia Committee of People's Repulic of China, 2020), which are accredited by the National Medical Products Administration, are produced and marketed in China to treat cough.However, further research is required to clarify any potential synergisms or interactions between the bioactive phytochemicals in P. praeruptorum and those of other medicinal plants, as well as to elucidate their mechanisms of action.According to the Chinese Pharmacopoeia 2020 (Chinese Pharmacopoeia Committee of People's Repulic of China, 2020), although Qianhu can disperse wind-heat, reduce cough and phlegm, and dissipate adverse Qi, comprehensive studies of its constitutive bioactive monomers should be conducted.

TABLE 3
Pharmacology of phytochemicals extracted from Peucedanum praeruptorum Dunn.