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ORIGINAL RESEARCH article
Front. Pharmacol.
Sec. Experimental Pharmacology and Drug Discovery
Volume 15 - 2024 |
doi: 10.3389/fphar.2024.1451695
This article is part of the Research Topic Pharmacological Mechanisms of Drugs Affecting Bone Formation and Bone Resorption Volume II View all 3 articles
Identification of miRNAs Related to Osteoporosis by Highthroughput Sequencing
Provisionally accepted- 1 Guangzhou University of Chinese Medicine, Guangzhou, China
- 2 The Third Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China
Background: Osteoporosis is a major health issue. MicroRNAs (miRNAs) play multiple roles in regulating cell growth and development. High-throughput sequencing technology is widely used nowadays.Objective: To screen for and validate miRNAs associated with osteoporosis.Method: Bone specimens from patients with (n=3) and without (n=3) osteoporosis were collected.High-throughput sequencing was used to screen for miRNAs that were then analyzed using volcano maps, Wayne maps, gene ontology (GO) analysis, and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis.Confirmation of the miRNAs was done using qRT-PCR. Results: The analysis of sequencing showed that there were 12 miRNAs that were down-regulated and five miRNAs that were upregulated in osteoporosis.GO and KEGG identified these miRNAs as being associated with bone metabolism.The qRT-PCR results showed that miR-140-5p, miR-127-3p, miR-199b-5p, miR-181a-5p, miR-181d-5p, and miR-542-3p exhibited a decrease of 2. 27-, 3.00-, 3.48-, 2.67-, 2.41-, and 1.98-fold (all P<0.05) in osteoporosis compared to controls. Conversely, miR-486-3p and miR-486-5p demonstrated an increase of 2.17-and 3.89-fold (P<0.05) (all P<0.05).This study utilized high-throughput sequencing to detect miRNAs that were expressed differently in individuals with osteoporosis.In osteoporosis, six miRNAs (miR-140-5p, miR-127-3p, miR-199b-5p, miR-181a-5p, miR-181d-5p, and miR-542) were found to have decreased expression, whereas two miRNAs (miR-486-3p and miR-486-5p) were found to have increased expression.The initial manifestation of various miRNAs might serve as predictive indicators and potentially anticipate the progression of osteoporosis.
Keywords: Osteoporosis, microRNA, Bone, High-throughout sequencing, qRT-PCR
Received: 19 Jun 2024; Accepted: 17 Jul 2024.
Copyright: © 2024 Yang, Huang, Chai, Lin, Huang, Zhang and Wan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Jiachun Huang, The Third Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China
Shuang Chai, Guangzhou University of Chinese Medicine, Guangzhou, China
Yanping Lin, The Third Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China
Hongxing Huang, The Third Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China
Zhihai Zhang, The Third Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China
Lei Wan, Guangzhou University of Chinese Medicine, Guangzhou, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Jiachun Huang
1,2*