AUTHOR=Tan Aditya , Scortecci Katia Castanho , Cabral De Medeiros Nathalia Maira , Kukula-Koch Wirginia , Butler Thomas J. , Smith Sinéad Marian , Boylan Fabio 

TITLE=Plukenetia volubilis leaves as source of anti-Helicobacter pylori agents

JOURNAL=Frontiers in Pharmacology

VOLUME=Volume 15 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1461447

DOI=10.3389/fphar.2024.1461447

ISSN=1663-9812

ABSTRACT=This piece of work showcases the plant Plukenetia volubilis as a source of pharmacologically active polyphenols. These compounds were identified by High-Performance Liquid Chromatography coupled to Electrospray Ionisation and Quadrupole Time-of-Flight Mass Spectrometry (HPLC-ESI-QTOF-MS-MS) analysis of its water infusion. HSCCC from the ethyl acetate extract of the water infusion allowed for the separation and structure characterisation of Astragalin. In vitro testing with the water extract, the ethyl acetate extract and Astragalin demonstrated the anti-Helicobacter pylori activity using a clarithromycin-resistant clinical isolate (MIC = 0.53, 0.51 qnd 0.49 µg/mL, respectively). Moreover in-silico analysis for astragalin confirmed its strong interactions with target proteins for H. pylori.