AUTHOR=Wang Wenjuan , Liu Hong , Wen Yongqing , Zhang Yanhua , Ma Xu 

TITLE=An integrated approach based on FDA adverse event reporting system, network pharmacology, molecular docking, and molecular dynamics simulation analysis to study the cardiac adverse reactions and mechanism of action of osimertinib

JOURNAL=Frontiers in Pharmacology

VOLUME=Volume 16 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1619517

DOI=10.3389/fphar.2025.1619517

ISSN=1663-9812

ABSTRACT=ObjectivesThis study investigates osimertinib-induced cardiac adverse reactions (CAR) using real-world FDA Adverse Event Reporting System (FAERS) data and explores molecular mechanisms via network pharmacology, molecular docking, and dynamics simulations.MethodsWe analyzed osimertinib-related adverse events from Q4 2015 to Q4 2024 using FAERS data, applying reporting odds ratio (ROR) and Bayesian confidence propagation neural network (BCPNN) methods. Potential CAR targets were identified via PharmMapper, Swiss Target Prediction, and GeneCards. Protein-protein interaction (PPI) networks, gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, molecular docking, and dynamics simulations were performed.ResultsAmong 15,382 reports, 274 were CAR-related, including pericardial effusion, cardiomyopathy, and cardiac dysfunction (25.00% mortality). Key targets (AKT1, ESR1, EGFR, SRC, ALB, CASP3) and pathways (PI3K-Akt, Ras, MAPK, calcium, JAK-STAT, TNF) were identified. Molecular docking confirmed strong binding affinity with binding energies below −7.5 kJ/mol for key targets (AKT1: -9.9 kJ/mol; ALB: −8.4 kJ/mol). Molecular dynamics simulations (100 ns) demonstrated stable binding of osimertinib-AKT1/ALB complexes, with average RMSD values of 0.52 nm and 0.50 nm, respectively, and binding free energies of −44.63 kJ/mol (AKT1) and −42.92 kJ/mol (ALB).ConclusionThis study clarifies osimertinib-induced CAR mechanisms involving multi-target interactions and pathway dysregulation, aiding clinical safety and future research.