AUTHOR=Lin Xinda , Zhang Ling , Jiang Yanyun 

TITLE=Distinct Roles of Met and Interacting Proteins on the Expressions of takeout Family Genes in Brown Planthopper

JOURNAL=Frontiers in Physiology

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2017.00100

DOI=10.3389/fphys.2017.00100

ISSN=1664-042X

ABSTRACT=<p>The <italic>takeout</italic> family genes encode relatively small proteins that are related to olfaction and are regulated by juvenile hormone (JH). The <italic>takeout</italic> genes modulate various physiological processes, such as behavioral plasticity in the migratory locust <italic>Locusta migraloria</italic> and feeding and courtship behaviors in <italic>Drosophila</italic>. Therefore, to understand the regulatory mechanism of these physiological processes, it is important to study the expressions of the <italic>takeout</italic> genes that are regulated by JH signaling. We used quantitative real-time PCR (qRTPCR) to study the role of JH signaling in the regulation of the <italic>takeout</italic> family genes in the brown planthopper <italic>Nilaparvata lugens</italic> (<italic>N. lugens</italic>) through the application of Juvenile hormone III (JHIII) and the down-regulation of key genes in the JH signaling pathway. The topical application of JHIII induced the expressions of most of the <italic>takeout</italic> family genes, and their expressions decreased 2 and 3 days after the JHIII application. Down-regulating the brown planthopper JH receptor NlMethoprene-tolerant (NlMet) and its interacting partners, NlTaiman (NlTai) and Nlß-Ftz-F1 (Nlß-Ftz), through RNAi, exhibited distinct effects on the expressions of the <italic>takeout</italic> family genes. The down-regulation of <italic>NlMet</italic> and <italic>NlKrüppel-homolog 1</italic> (<italic>NlKr-h1</italic>) increased the expressions of the <italic>takeout</italic> family genes, while the down-regulation of the Met interacting partners <italic>NlTai</italic> and <italic>Nlß-Ftz</italic> decreased the expressions of most of the <italic>takeout</italic> family genes. This work advanced our understanding of the molecular function and the regulatory mechanism of JH signaling.</p>