AUTHOR=Yokota Rodrigo , Ronchi Fernanda Aparecida , Fernandes Fernanda Barrinha , Jara Zaira Palomino , Rosa Rodolfo Mattar , Leite Ana Paula de Oliveira , Fiorino Patricia , Farah Vera , Nascimento Nilberto Robson Falcão do , Fonteles Manassés C. , Casarini Dulce Elena 

TITLE=Intra-Renal Angiotensin Levels Are Increased in High-Fructose Fed Rats in the Extracorporeal Renal Perfusion Model

JOURNAL=Frontiers in Physiology

VOLUME=Volume 9 - 2018

YEAR=2018

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2018.01433

DOI=10.3389/fphys.2018.01433

ISSN=1664-042X

ABSTRACT=Overconsumption of fructose leads to metabolic syndrome as a result of
hypertension, insulin resistance, and hyperlipidemia. In this study, the renal
function of animals submitted to high fructose intake was analyzed from weaning
to adulthood using in-vivo and ex-vivo methods, being compared with a normal
control group. We investigated in ex-vivo model of the role of the renin angiotensin
system (RAS) in the kidney. The use of perfused kidney from animals submitted to
8-week fructose treatment showed that high fructose intake caused metabolic and
cardiovascular alterations that were consistent with other studies. Moreover, the
isolated perfused kidneys obtained from rats under high fructose diet showed a
33% increase in renal perfusion pressure throughout the experimental period due
to increased renal vascular resistance and a progressive fall in the glomerular
filtration rate, which reached a maximum of 64% decrease. Analysis of RAS
peptides in the high fructose group showed a three-fold increase in the renal
concentrations of angiotensin I and a two-fold increase in angiotensin II levels,
whereas no change in angiotensin 1-7 was observed when compared with the
control animals. We detected a decrease in ACE activity in renal tissues, but high
concentrations of angiotensin II. These observations suggest that alternative
pathways for angiotensin II production exist in this model. Chymase, tonin, and
cathepsin are possible enzymes responsible for the observed angiotensin II
increase. The increased production of the intrinsic renal angiotensin I and II in
high-fructose fed rats led to adaptive renal function changes that are probably
associated with both increased blood pressure in these animals and kidney
damage associated with this model.