AUTHOR=Sun Jing-yi , Hou Ya-jun , Fu Xiao-yan , Fu Xiao-ting , Ma Jin-kui , Yang Ming-feng , Sun Bao-liang , Fan Cun-dong , Oh Jinrok 

TITLE=Selenium-Containing Protein From Selenium-Enriched Spirulina platensis Attenuates Cisplatin-Induced Apoptosis in MC3T3-E1 Mouse Preosteoblast by Inhibiting Mitochondrial Dysfunction and ROS-Mediated Oxidative Damage

JOURNAL=Frontiers in Physiology

VOLUME=Volume 9 - 2018

YEAR=2019

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2018.01907

DOI=10.3389/fphys.2018.01907

ISSN=1664-042X

ABSTRACT=Selenium-containing protein from selenium-enriched S. platensis (Se-SP) exhibited novel hepatoprotective potential through inhibiting oxidative damage. However, the molecular mechanism remains unclear. Herein, the protective effect of Se-SP against cisplatin-induced oxidative damage and apoptosis in MC3T3-E1 mouse preosteoblast was investigated, and the underlying mechanism was evaluated. The results showed that cisplatin treatment significantly inhibited cell viability of preosteoblast by triggering mitochondria-mediated apoptosis pathway. Cisplatin treatment also caused mitochondrial dysfunction and reactive oxide species (ROS)-mediated oxidative damage. However, Se-SP pre-treatment significantly blocked cisplatin-induced mitochondrial dysfunction by balancing Bcl-2 family expression and regulating the opening of mitochondrial permeability transition pore (MPTP), attenuated cisplatin-induced oxidative damage through inhibiting the overproduction of ROS and superoxide anion, and eventually reversed cisplating-induced apoptosis by inhibiting PARP cleavage and caspases activation. Our findings validated the strategy of using Se-SP as a promising Se species could be a highly effective way in combating oxidative damage-mediated diseases.