AUTHOR=Li Dan-Dan , Ling Shi-Cheng , Wu Kun , Luo Zhi 

TITLE=Identification of Five Key Genes Involved in Intrinsic Apoptotic Pathway From Yellow Catfish Pelteobagrus fulvidraco and Their Transcriptional Responses to High Fat Diet (HFD)

JOURNAL=Frontiers in Physiology

VOLUME=Volume 10 - 2019

YEAR=2019

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2019.00921

DOI=10.3389/fphys.2019.00921

ISSN=1664-042X

ABSTRACT=The hypothesis of the present study is that apoptosis through intrinsic mitochondrial pathway may mediate high fat diet (HFD)-induced changes of metabolism of P. fulvidraco. To this end, we cloned the full-length cDNA sequences of Cycs, Apaf1, Casp9, Casp3a and Casp3b involved in mitochondria apoptotic pathway, and explored their mRNA tissue expressions and transcriptional responses to HFD. All of these members shared similar domains to their orthologous genes of vertebrates. They were constitutively expressed in all analyzed tissues, but varied from tissue to tissue. Compared to the control, HFD up-regulated the mRNA expression of partial genes among these five key genes (Cycs, Apaf1, Casp9, Casp3a and Casp3b) in mesenteric fat, intestine, ovary and kidney, indicating the induction of apoptosis in these tissues; in contrast, HFD down-regulated mRNA levels of partial genes among the five key genes (Cycs, Apaf1, Casp9, Casp3a and Casp3b) in heart, spleen and gill, indicating the inhibition of apoptosis in these tissues. The present study will facilitate further exploration into their functions of these genes at the molecular level and disclose the critical involvement of these genes against nutrient changes, indicating that the processes of apoptosis in various tissues may differentially be modified by HFD.