AUTHOR=Johansen Jan-Michael , Goleva-Fjellet Sannija , Sunde Arnstein , Gjerløw Lars Erik , Skeimo Lars Arne , Freberg Baard I. , Sæbø Mona , Helgerud Jan , Støren Øyvind TITLE=No Change – No Gain; The Effect of Age, Sex, Selected Genes and Training on Physiological and Performance Adaptations in Cross-Country Skiing JOURNAL=Frontiers in Physiology VOLUME=Volume 11 - 2020 YEAR=2020 URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2020.581339 DOI=10.3389/fphys.2020.581339 ISSN=1664-042X ABSTRACT=The aim was to investigate the effect of training, sex, age and selected genes on physiological and performance variables and adaptations before, and during six months of training in well-trained cross-country skiers. In order to do this we recruited 46 national-level cross-country skiers for a six months observational study (pre – post 1 - post 2 test). All participants were tested in a double poling time trial (TTDP) maximal oxygen uptake in running (RUN-VO2max), peak oxygen uptake in double poling (DP-VO2peak), lactate threshold in double poling (LT), oxygen cost of double poling (CDP), jump height and maximal strength (1RM) in half squat and pull-down. At baseline, blood samples were drawn to investigate the genetic status of the ACTN3 R577X, ACE I/D, PPARGC1A rs8192678, PPARG rs1801282, PPARA rs4253778, ACSL1 rs6552828 and IL6 rs1474347 polymorphisms. The skiers were instructed to train as normal and report all training in training diaries based on heart rate measures from May to October. 29 skiers completed all testing and registered their training sufficiently throughout the study period. At pre-test, significant sex and age differences were observed in TTDP (p < 0.01), DP-VO2peak (p < 0.01), CDP (p < 0.05), MAS (p < 0.01), LTv (p < 0.01), 1RM half squat (p < 0.01) and 1RM pull-down (p < 0.01). For sex, there was also a significant difference in RUN-VO2max (p < 0.01). No major differences were detected in physiological or performance variables based on genotypes. The training characteristics of the included skiers were in accordance with general recommendations for endurance performance development. Total training volume and ski-specific training increased significantly (p < 0.05) throughout the study period for the whole group, while the training intensity distribution was maintained. No physiological or performance variables improved during the six months of training for the whole group. No differences were observed in training progression or adaptation between sex or age-groups. In conclusion, sex and age affected physiological and performance variables, with only a minor impact from selected genes, at baseline, but not on training adaptations. Increased total training volume did not affect physiological and performance variables.