The first step to resolve to conflict between the “membrane” and “metabolic” hypotheses came from experiments on PC12 cells (an O₂-sensitive catecholaminergic cell line; Taylor et al., 2000) and carotid body slices (Ortega-Saenz et al., 2003) showing that, as it occurs with hypoxia, catecholamine secretion induced by mitochondrial electron transport chain (ETC) inhibitors acting on complexes I, II, III, and IV is fully abolished by removal of extracellular Ca²⁺ or administration of 0.2 mM Cd²⁺, a non-selective voltage-gated Ca²⁺ channel blocker (Urena et al., 1994). Separate experiments showed that ETC blockers also inhibit the O₂-sensitive background K⁺ current in dispersed glomus cells (Wyatt and Buckler, 2004). These data on single cells are in good agreement by previous work on whole CBs showing that dopamine secretion during incubation with cyanide is strongly inhibited in the absence of extracellular Ca²⁺ (Obeso et al., 1989). Ortega-Saenz et al. (2003) found that rotenone, a highly selective mitochondria complex I (MCI) blocker that binds near the last Fe/S cluster (N2 site) and prevents the transfer of electrons to ubiquinone, was very effective in occluding any effect of hypoxia. In contrast, activation of glomus cells by hypoglycemia was unaffected by rotenone (Garcia-Fernandez et al., 2007). These data suggested that hypoxia and hypoglycemia are sensed by separate mechanisms and that a rotenone binding site is directly involved in acute O₂ sensing by glomus cells.

To investigate the role of MCI in acute O₂ sensing, we generated conditional knockout mice lacking the Ndufs2 gene, which codes a 49 kDa protein that contributes to the ubiquinone/rotenone binding site and is also essential for the assembly of the catalytic core in MCI (Kashani-Poor et al., 2001; Carroll et al., 2013). Because generalized bi-allelic deletion of Ndufs2 results in embryonic lethality, we generated conditional Ndufs2 knockout mice with either ablation of Ndufs2 in glomus cells and other catecholaminergic cells (TH-NDUFS2 mice) or ubiquitous tamoxifen (TMX)-induced Ndufs2 deletion in adulthood (ESR-NDUFS2 mice). TH-NDUFS2 mice had a normal development although they had smaller size than adult wild type littermates probably due to dwarfing secondary to the loss of hypothalamic dopaminergic neurons (Diaz-Castro et al., 2012; Fernandez-Aguera et al., 2015). At 2 months of age, these mice exhibited a loss of the hypoxic ventilatory response (HVR; Figure 2A), although they had a normal ventilatory response to hypercapnia (Fernandez-Aguera et al., 2015). CBs from TH-NDUFS2 mice appeared slightly hypertrophied and with normal structural organization. However, Ndufs2-deficient glomus cells showed an almost complete abolition of responsiveness to hypoxia (monitored by either the catecholamine secretory response or the changes in cytosolic [Ca²⁺]), while they responded normally to hypercapnia and hypoglycemia (Figure 2B). Similar results were observed in ESR-NDUFS2 mice, in which Ndufs2 deficiency was induced by TMX treatment in adulthood and exhibited an almost complete disappearance of MCI structure and function (Fernandez-Aguera et al., 2015; Arias-Mayenco et al., 2018). In contrast with the effects of Ndufs2 deficiency, ablation of the Ndufs4 gene, which codes a non-essential MCI auxiliary subunit that reduces MCI activity by approximately 50% (Kruse et al., 2008), did not cause appreciable changes in the catecholamine release and cytosolic Ca²⁺ responses to hypoxia in glomus cells (Fernandez-Aguera et al., 2015). These data indicated that MCI function is essential for acute O₂ sensing and confirmed that hypoxia and hypoglycemia are sensed by means of separate mechanisms. Interestingly, it was found that CB cells contain high levels of succinate, suggesting a highly active Kreb’s cycle, and that upregulation or downregulation of succinate dehydrogenase activity enhances or diminishes, respectively, sensitivity to hypoxia in glomus cells (Fernandez-Aguera et al., 2015; Gao et al., 2017; Arias-Mayenco et al., 2018). Although glomus cells survived several months in the absence of MCI, they rapidly died after ablation of MCII (Diaz-Castro et al., 2012; Platero-Luengo et al., 2014).

Taken together, these experimental findings suggested a model of acute O₂ sensing in which mitochondria, acting as a sensor and effector of the hypoxic response, modulate membrane excitability. We proposed that decreased cytochrome c oxidase activity under hypoxia causes a backlog of electrons along the ETC and an increase in the ratio of reduced/oxidized ubiquinone (QH₂/Q), which results in slowing down or even reversion of MCI with NADH accumulation and reactive oxygen species (ROS) production (Figure 2C). NADH and ROS are the signals that modulate plasmalemmal ion channels to produce depolarization and activation of glomus cells. Graded accumulation of NADH in glomus cells induced by lowering PO₂ was abolished in Ndufs2-deficient mice (Fernandez-Aguera et al., 2015; Arias-Mayenco et al., 2018; Figure 2D). We were also able to monitor in real time the changes in mitochondrial ROS production by means of a fluorescent genetic probe targeted to either mitochondrial intermembrane space (IMS) or matrix. Using this methodology, we demonstrated that acute hypoxia induces in glomus cells a dose-dependent increase in IMS (and cytosol) ROS, which is markedly decreased by rotenone and in Ndufs2-deficient mice (Figure 2E). However, the possibility that IMS ROS produced in other sites along a reduced ETC (e.g., in MCIII) also contribute to the hypoxic response cannot be discarded (Figure 2C; Waypa et al., 2010). In support of the MMS model, we showed that intracellular dialysis of glomus cells with NADH and H₂O₂ mimic hypoxia (increase in input resistance and decrease in voltage-gated K⁺ current amplitude) and prevents further modulation of K⁺ channels by lowering PO₂ (Fernandez-Aguera et al., 2015). Other mitochondrial signals (e.g., decrease in cytosolic ATP level restricted to O₂-sensing microdomains; see below) could also contribute to modulation of membrane channels and the hypoxic response (Varas et al., 2007).

In the past decades, several groups have reported gene expression data focusing on different aspects of CB glomus cell function and, recently, two such studies provided relevant clues for advancing the understanding of glomus cell acute O₂ sensing. In one case, single neonatal glomus cell RNA sequencing confirmed the constitutive high expression of Hif2α and highlighted the elevated expression of two atypical mitochondrial subunits (Ndufa4l2 and Cox4i2), and several ion channels, in particular, Task1 and the low-threshold Ca²⁺ channel α1H subunit (Zhou et al., 2016). This work also showed the high level of expression of genes coding for molecules involved in G-protein signaling, an observation compatible with the elevated number of metabotropic ligands and receptors in glomus cells. A parallel microarray study performed in our laboratory focused on the comparative expression profile of adult CB, adrenal medulla (AM), and superior cervical ganglion (SCG), which are tissues of the same neural crest embryological origin but variable O₂ sensitivity (CB>AM>SCG). Our work confirmed most of the genes reported in the single-cell sequencing study mentioned above and demonstrated a set of genes highly expressed in CB, and less markedly in the AM, in comparison with the SCG with a potential role in acute O₂ sensing (Gao et al., 2017). The most relevant genes in the CB signature gene expression profile code Hif2α, three atypical mitochondrial subunits (Ndufa4l2, Cox4i2, and Cox8b), pyruvate carboxylase (Pcx), and some types of ion channels (Task1, Task3, and the α1H Ca²⁺ channel subunit). In the context of the MMS model, it was of special relevance the identification of Pcx and the three nuclear-encoded atypical mitochondrial subunits (Ndufa4l2, Cox4i2, and Cox8b), which could be responsible for the special O₂-sensitivity of glomus cells. In particular, the high level of Pcx mRNA expression is compatible with the accumulation of biotin, a cofactor necessary for the function of Pcx and other carboxylases, accumulated in large quantity in glomus cells (Ortega-Saenz et al., 2016). Pcx is an anaplerotic enzyme that catalyzes the formation of oxaloacetate, thereby replenishing the pool of Krebs’s cycle intermediates required for an accelerated synthesis of substrates (NADH and FADH₂) for the ETC. Therefore, Pcx probably contributes to the active oxidative metabolism and high O₂ consumption characteristic of CB cells. This idea is also compatible with the high levels of succinate found in the CB and the strict dependence of CB survival and function on succinate dehydrogenase activity (Diaz-Castro et al., 2012; Platero-Luengo et al., 2014; Fernandez-Aguera et al., 2015).

Although it was known long ago that Hif2α is constitutively expressed at high levels in normoxic catecholaminergic tissues (Tian et al., 1998), the role of this factor in CB function has not been studied until the last few years. It has been shown that transgenic overexpression of Epas1 (the gene coding Hif2α) produces CB hypertrophy (Macias et al., 2014) and embryonic ablation of Epas1 results in CB atrophy (Macias et al., 2018), thereby suggesting that Hif2α is essential for CB development and function. Heterozygous (Epas1+/−) mice were reported to have an exaggerated CB responsiveness to hypoxia (Peng et al., 2011) but more recent experiments performed independently by two different groups have shown that these mice have a decrease in the HVR (Hodson et al., 2016; Moreno-Dominguez et al., 2020). Inhibition of the HVR is also seen in variable degrees in mice with homozygous partial (Hodson et al., 2016) or complete (Moreno-Dominguez et al., 2020) conditional deletion of Epas1 in adulthood. In agreement with these observations, glomus cells from conditional Epas1-null mice show selective abolition of the rise in cytosolic [Ca²⁺] (Figure 3A, left and center) or the secretory response to hypoxia. Moreover, NADH and IMS ROS signals induced by hypoxia are strongly inhibited in Epas1-deficient glomus cells (Figures 3B,C). Interestingly, the hypoxia-induced decrease in matrix ROS (Arias-Mayenco et al., 2018) was not altered by Epas1 deficiency (Figure 3D) thereby indicating that the lack of Hif2α did not change the basic mitochondria metabolism but selectively inhibited signaling in response to low PO₂. In parallel with these functional data, it has been shown that abolition of Epas1 results in a selective downregulation of mRNAs coding Pcx and the atypical mitochondrial ETC subunits characteristic of CB cells (Moreno-Dominguez et al., 2020). These results are compatible with previous studies reporting that hypoxia induces Cox4i2 (Fukuda et al., 2007) and Ndufa4l2 (Tello et al., 2011) in a Hif-dependent manner (see also Aras et al., 2013), and that Cox8b promoter contains Hif binding sites (Gao et al., 2017). Together these findings indicate that the expression of Hif2α-dependent genes confer acute O₂ responsiveness to CB glomus cells. Indeed, the Epas1-null phenotype (inhibition of HVR and lack of glomus cells sensitivity to hypoxia) is also observed in mice with ablation of the Cox4i2 gene in catecholaminergic cells (TH-COX4I2 mice; Figure 3A, right; Moreno-Dominguez et al., 2020).

The data reported so far provide molecular and mechanistic explanation for an MMS model of acute O₂ sensing by arterial chemoreceptor cells in which cytochrome c oxidase acts as an O₂ sensor that, depending on O₂ availability, determines the redox state of the steps upstream in the ETC. In response to hypoxia, the increase in the reduced state of MCIII and accumulation of QH₂ results in the generation of the signals (NADH and ROS) that modulate membrane ion channels (Figures 4A,B, see also Figure 2C). However, the precise role of each of the atypical MCIV subunits and how they influence acute O₂ sensing in the CB and, possibly, other acutely responding organs, remains to be studied. Ndufa4l2, which is coded by one of the most abundant mRNA species in CB glomus cells, is an isoform of the most widely expressed Ndufa4 subunit, which appears to be associated to MCIV rather than to MCI (Carroll et al., 2006; Balsa et al., 2012). Ndufa4l2 is highly expressed in lung and brain pericytes and some tumor cells (Lucarelli et al., 2018) but its function is poorly known. Expression of Ndufa4l2 attenuates oxygen consumption and decreases ROS production in mitochondria (Tello et al., 2011; Meng et al., 2019), however ablation of the Ndufa4l2 gene did not produce any clear effect on glomus cell function or HVR (Moreno-Dominguez et al., 2020). Therefore, the precise role of Ndufa4l2 in the context of acute O₂ sensing remains to be determined. On the other hand, Cox4i2 and Cox8b are atypical isoforms of the more broadly distributed Cox4i1 and Cox8a subunits, which are part of the catalytic core of MCIV (Tsukihara et al., 1996). Besides in the CB, Cox4i2 is highly expressed in the lung and some cell types (e.g., pericytes; Huttemann et al., 2012) and Cox8b appears associated to the browning of adipose tissue (Wang et al., 2016). Interestingly, Cox4 and Cox8 subunits contain single adjacent transmembrane helices running in parallel at the periphery of MCIV (Tsukihara et al., 1996; Kadenbach and Huttemann, 2015) that, although located relatively far from the catalytic site (heme a3/CuB), could induce subtle structural changes in MCIV or in its association with supercomplexes that influence the affinity for or reaction rate with O₂. Structural studies have suggested that the Cox8 subunit contributes to the formation of mitochondrial supercomplexes (Wu et al., 2016; Rieger et al., 2017) and a recent study on tumor cells lines have reported that expression of Cox4i2 (instead Cox4i1) decreases the Km of cytochrome c oxidase for O₂ (Pajuelo Reguera et al., 2020). In this last study, the Km of cytochrome c oxidase for O₂ varied between ~0.5 mm Hg (in mitochondria expressing Cox4i1) and ~1 mm Hg (in mitochondria expressing Cox4i2). These are PO₂ values much lower than those necessary for activation of glomus cells, even assuming a steep O₂ gradient between the extracellular medium and mitochondria. Therefore, it seems that in addition to the Cox4 subunit isoforms, other factors may influence the Km of cytochrome c oxidase for O₂ in glomus cells. In sum, the MMS model provides a satisfactory molecular explanation for acute O₂ sensing by arterial chemoreceptor cells, which depends on a Hif2α-dependent expression of specific genes. The special O₂ sensitivity of glomus cells seems to result from the combination of an accelerated ETC and O₂ consumption due to an active Krebs cycle and a relatively low affinity of cytochrome c oxidase for O₂. In this way, electron flux in mitochondrial ETC is modulated in a physiological range of O₂ tensions. Whether an MMS model, involving similar genes and regulatory mechanisms, also participates in acute O₂ sensing by other tissues remains to be studied. In this regard, it is important to note that Cox4i2-deficient mice exhibit strong inhibition of hypoxic pulmonary vasoconstriction (Sommer et al., 2017), an acute response to hypoxia that, similar to hypoxic glomus cell activation, depends on the production of ROS by mitochondria and the modulation of O₂-sensitive K⁺ channels (Weir et al., 2005).

CB activation is the first line of defense against hypoxic challenges and, therefore, CB dysfunction may have fatal consequences. Indeed, bilateral resection of the CB, most commonly due neck tumor surgery or asthma treatment, leaves the patients unaware of hypoxemia (Timmers et al., 2003). These patients cannot adapt to hypoxic environments and although they appear to live unaffected in normoxic conditions, disturbances during sleep and unexplained cases of death have been reported. Genetic/developmental CB defects, such as congenital central hypoventilation syndrome (CCHS) and sudden infant death syndrome (SIDS) are life-threatening disorders partially related to alterations in CB function that, although rare in humans, can seriously impair O₂-dependent respiratory control. CCHS is frequently associated with mutations in genes (such as RET or PHOX2B), which are relevant to development of neural crest-derived tissues (Amiel et al., 2003; Gaultier et al., 2004). Interestingly, CB glomus cells express high levels of GDNF (Villadiego et al., 2005), a neuroprotective dopaminotrophic factor that can activate RET, and genetic ablation of GDNF in adulthood results in a marked reduction in the number of TH-positive cells in the CB (Pascual et al., 2008). Decrease in size with reduction in the number of TH-positive cells and increased number of type II cells has been reported in CBs of autopsied CCHS (Cutz et al., 1997) and SIDS (Porzionato et al., 2013) patients. Prematurity and environmental factors, such as hyperoxia, retard maturation of CB chemoreceptors. Maternal smoking inhibits CB development and the excitability of AM chromaffin cells (Buttigieg et al., 2009).

The most frequent cause of CB inhibition is the use (or abuse) of anesthetics, myorelaxants, and analgesics. Volatile anesthetics (halothane and others) depress glomus cell excitability because they increase the open probability of background TASK1-like K⁺ channels (Buckler et al., 2000). Most of the myorelaxant drugs used in anesthesia are cholinergic antagonists, which interfere with the activation of the CB chemosensory synapse and inhibit the hypoxic ventilatory response (Jonsson et al., 2004). Endogenous opioids (enkephalins) are produced in the CB, where they have an auto‐ or paracrine inhibitory effect (Kirby and McQueen, 1986). Systemic administration of opioids produces a strong respiratory depression, due in part to inhibition of peripheral chemoreceptors (Pokorski and Lahiri, 1981). However, opioid-induced respiratory depression (OIRD) in conscious rats is enhanced after bilateral CB denervation, suggesting a protective rather than causative role of the CB in OIRD (Baby et al., 2018). The design of well-tolerated drugs to activate peripheral chemoreceptors, which in turn stimulate the respiratory center, is a promising strategy to alleviate OIRD in humans; a clinical condition that has become a major health problem, particularly in the United States with a toll of over 150 deaths daily. In this regard, blockers of several types of K⁺ channels are already being tested in the clinical setting as respiratory stimulants (Chokshi et al., 2015; Roozekrans et al., 2015). Within the context of this discussion, it is worth mentioning that these CB stimulants, which act downstream of the O₂-sensing mechanism, might be useful to treat “silent hypoxemia,” a bewildering frequent clinical manifestation found in patients with coronavirus disease 19 (COVID-19), who exhibit severe hypoxemia without clear signs of distress (dyspnea) or significant acceleration of breathing (Tobin et al., 2020). Given that in the early stages of coronavirus infection human cells undergo profound changes in the expression of mitochondrial proteins (Gordon et al., 2020), a plausible explanation for “silent hypoxemia” is the alteration of the mitochondria-based O₂-sensor in coronavirus-infected CB glomus cells (Archer et al., 2020; Tobin et al., 2020). Another aspect of the MMS model of acute O₂ sensing with translational relevance is the identification of the mitochondrial ETC as a potential pharmacological target to stimulate respiration. In this regard, it should be tested whether MCI inhibitors, such as metformin, one of the most broadly used drugs to treat type II diabetes (Protti, 2018), can activate the CB and stimulate respiration. It could be optimal to combine metformin with novel highly membrane permeant precursors of succinate (bis-1-acetoxy-ethyl succinate or diacetoxy-methyl succinate; Ehinger et al., 2016). We have shown that increased levels of ubiquinol (CoQH₂) resulting from the application of membrane permeant dimethyl succinate increases responsiveness to hypoxia (Arias-Mayenco et al., 2018). A combination of both therapies (metformin plus succinate prodrugs) would potentiate CB activation and at the same time prevent lactic acidosis secondary to metformin seen in some patients (Protti, 2018).

Chronic activation of the CB, as it occurs in patients with sleep apnea, metabolic syndrome or chronic left cardiac failure, due to intermittent hypoxia, high fat diet, or carotid hypoperfusion, respectively, can lead to exaggerated sympathetic outflow and autonomic dysfunction (see for review Ortega-Saenz and Lopez-Barneo, 2020). Although the pathophysiology of these maladaptive processes is still poorly known (Marcus et al., 2010; Paton et al., 2013; Schultz et al., 2013; Ribeiro et al., 2018), it has been shown in animal models that CB resection or deafferentation restores the sympathetic tone and improves the associated cardiovascular and metabolic alterations (Del Rio et al., 2013, 2016; McBryde et al., 2013; Ribeiro et al., 2013). However, translation of this therapy to the clinical setting has numerous limitations because the lack of CB may be cause of cardiovascular events, particularly during episodes of hypoxia and hypercapnia. CB resection could also have severe side effects such as altered glucose regulation or a reduced ability to acclimatize to high altitudes (Johnson and Joyner, 2013; Pijacka et al., 2018). In a pilot clinical trial performed on patients with chronic heart failure, bilateral CB ablation improved the autonomic imbalance but also increased the occurrence of nocturnal hypoxia, particularly in subjects with concomitant sleep apnea (Niewinski et al., 2017). An alternative to CB resection is the development of pharmacological drugs to selectively modulate CB chemosensory activity and plasticity. In this regard, it has been shown that a purinergic P2X3 receptor blocker (AF-219) inhibits CB afferent activity and alleviates hypertension in a rat model (Pijacka et al., 2016). The translation of these findings to the clinical setting may be facilitated by the fact that purinergic P2X3 receptor blockers (i.e., AF-219; also known as MK-7264 or Gefapixant) are already used in clinical trials to treat refractory chronic cough in humans, notwithstanding the unwanted side effect that taste sensation is also affected (Smith et al., 2020). A novel potential therapeutic option is represented by Hif2 antagonists, drugs already in clinical trials for the treatment of some types of cancer (Courtney et al., 2018; Fallah and Rini, 2019). Acute O₂ sensing by glomus cells depends on Hif2α (Moreno-Dominguez et al., 2020) and systemic administration of a Hif2 inhibitor (PT2385) results in inhibition of the HVR (Cheng et al., 2020). Moreover, Hif2α is necessary for the proliferation of CB cells in hypoxia (Hodson et al., 2016) and activation of glomus cells is necessary for the proliferation and differentiation of CB progenitors and neuroblasts into mature O₂-sensitive glomus cells (Platero-Luengo et al., 2014; Sobrino et al., 2018). Hence, Hif2 inhibitors may be beneficial to selectively modulate CB responsiveness to hypoxia and sympathetic over-activation.

Chemodectomas are rare and mostly benign CB tumors that have attracted special attention because they are often used as a model to investigate the pathogenesis of paragangliomas (PGL), tumors generated in tissues of the peripheral nervous system derived from neural crest precursors. The most common cause of hereditary CB PGL is germ line mutations in genes coding subunits of mitochondrial succinate dehydrogenase (most frequently mutations in Sdhd and Sdhc; Baysal, 2008; Her and Maher, 2015). Patients are heterozygous (with a normal and a mutated allele) and tumorigenesis is believed to be triggered by the loss of the normal allele (loss of heterozygosity) in CB glomus cells. However, the reasons why this allele is lost in some cell types (e.g., cells in CB and other paraganglia) and not in others as well as the mechanisms leading to tumor formation are unknown (Millan-Ucles et al., 2014). Given that the histology of CB PGL resembles that of hypertrophied CBs seen in chronically hypoxic subjects and that PGL incidence increases in populations living at high altitude (Astrom et al., 2003), a widely accepted hypothesis of tumor generation is the so called “pseudo hypoxic drive” (Selak et al., 2005; Smith et al., 2007). This hypothesis is based on the fact that succinate accumulation, secondary to succinate dehydrogenase dysfunction, causes downstream inhibition of prolyl hydroxylases involved in normal degradation of Hif as well as inhibition of histone demethylases and other enzymes, thereby causing cell proliferation. Indeed, overexpression of nondegradable Hif2α (but not Hif1α) induces CB hypertrophy (Macias et al., 2014). Moreover, deletion of the gene coding for prolyl hydroxylase 2 in mice induces Hif2α-dependent CB glomus cell proliferation with a PGL-like phenotype (Fielding et al., 2018). However, experimental evidence indicates that unlike humans, heterozygosity for mutations in succinate dehydrogenase subunits does not predispose mice to PGL. Adult knockout mice heterozygous for Sdhd show practically normal CB function, with only a subtle glomus cell hyperplasia and organ hypertrophy (Piruat et al., 2004). In addition, conditional (embryonic or adult) bi-allelic ablation of Sdhd causes a marked glomus cell loss (Diaz-Castro et al., 2012). It seems therefore that in addition to succinate dehydrogenase subunit mutations, other hits, related to animal species, age, or cell metabolism, are necessary for tumorigenesis in vivo. Although there is convincing in vitro and in vivo evidence that multipotent stem cells contribute to CB angiogenesis and expansion of parenchyma during exposure to sustained hypoxia (Pardal et al., 2007; Annese et al., 2017), it has also been shown that proliferation of TH-positive cells greatly contributes to the growth of the glomus cell pool during the first 2–3 days of hypoxia (Paciga et al., 1999; Chen et al., 2007; Wang et al., 2008; Fielding et al., 2018). In the rat, and probably also in other species, this initial glomus cell expansion is due to proliferation and maturation of a population of TH-positive neuroblasts, which differentiate into O₂-sensing glomus cells (Sobrino et al., 2018). Because hypoxia does not seem to induce proliferation of CB stem cells and undifferentiated progenitors in vitro (Platero-Luengo et al., 2014), a fundamental question that remains to be answered is whether hypoxia-induced release of transmitter and cytokines by mature glomus cells is a critical paracrine signal to trigger CB TH-positive cell proliferation and possibly the initial stages of tumor transformation. This would explain why Hif2α stabilization increases CB growth and the expansion of TH-positive cell population, and it would also support the use of Hif2 antagonists as potential therapeutic options to prevent CB PGL formation and growth.

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.