AUTHOR=Ma Siqing , Xu Heng , Huang Weihua , Gao Yongchao , Zhou Honghao , Li Xiong , Zhang Wei 

TITLE=Chrysophanol Relieves Cisplatin-Induced Nephrotoxicity via Concomitant Inhibition of Oxidative Stress, Apoptosis, and Inflammation

JOURNAL=Frontiers in Physiology

VOLUME=Volume 12 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2021.706359

DOI=10.3389/fphys.2021.706359

ISSN=1664-042X

ABSTRACT=Cisplatin (DDP) is one of the most frequently prescribed chemotherapy medications. However, its nephrotoxicity which often leads to acute kidney injury (AKI), greatly limits its clinical application. Chrysophanol (CHR), a mainly active anthraquinone ingredient, possesses various biological activity and pharmacological activity. In this study, we aimed to investigate the underlying protective mechanisms of CHR against DDP-induced acute kidney injury (DDP-AKI) by using C57BL/6 mice and human proximal tubule epithelial cells. In vivo, we found that pre-treatment with CHR greatly relieved DDP-AKI and improved the kidney function and morphology. The mechanistic studies indicated that it might alleviated DDP-AKI by inhibiting oxidative stress, apoptosis and IKKβ/IκB𝛂/p65/NF-κB inflammation signaling pathway induced by DDP. Moreover, we found that the cell viability of HK2 cells reduced by DDP was partially rescued by CHR pre-incubation. Flow cytometry results further indicated that CHR pre-incubation suppressed DDP induced cellular ROS generation and inhibited cell apoptosis in a dose-dependent manner. In summary, our results suggested that CHR might be a novel therapy for DDP-induced acute kidney injury.