AUTHOR=Caldeira Dayene de Assis Fernandes , Oliveira Dahienne Ferreira de , Cavalcanti-de-Albuquerque João Paulo , Nascimento Jose Hamilton Matheus , Zin Walter Araujo , Maciel Leonardo TITLE=Isolation of Mitochondria From Fresh Mice Lung Tissue JOURNAL=Frontiers in Physiology VOLUME=12 YEAR=2021 URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2021.748261 DOI=10.3389/fphys.2021.748261 ISSN=1664-042X ABSTRACT=

Direct analysis of isolated mitochondria enables a better understanding of lung dysfunction. Despite well-defined mitochondrial isolation protocols applicable to other tissues, such as the brain, kidney, heart, and liver, a robust and reproductive protocol has not yet been advanced for the lung. We describe a protocol for the isolation of mitochondria from lung tissue aiming for functional analyses of mitochondrial O2 consumption, transmembrane potential, reactive oxygen species (ROS) formation, ATP production, and swelling. We compared our protocol to that used for heart mitochondrial function that is well-established in the literature, and achieved similar results.