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<front>
<journal-meta>
<journal-id journal-id-type="publisher-id">Front. Physiol.</journal-id>
<journal-title>Frontiers in Physiology</journal-title>
<abbrev-journal-title abbrev-type="pubmed">Front. Physiol.</abbrev-journal-title>
<issn pub-type="epub">1664-042X</issn>
<publisher>
<publisher-name>Frontiers Media S.A.</publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="publisher-id">1024418</article-id>
<article-id pub-id-type="doi">10.3389/fphys.2022.1024418</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Physiology</subject>
<subj-group>
<subject>Systematic Review</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group>
<article-title>Effects of plyometric training on skill and physical performance in healthy tennis players: A systematic review and meta-analysis</article-title>
<alt-title alt-title-type="left-running-head">Deng et al.</alt-title>
<alt-title alt-title-type="right-running-head">
<ext-link ext-link-type="uri" xlink:href="https://doi.org/10.3389/fphys.2022.1024418">10.3389/fphys.2022.1024418</ext-link>
</alt-title>
</title-group>
<contrib-group>
<contrib contrib-type="author" corresp="yes">
<name>
<surname>Deng</surname>
<given-names>Nuannuan</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
<xref ref-type="corresp" rid="c001">&#x2a;</xref>
<uri xlink:href="https://loop.frontiersin.org/people/1922680/overview"/>
</contrib>
<contrib contrib-type="author" corresp="yes">
<name>
<surname>Soh</surname>
<given-names>Kim Geok</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
<xref ref-type="corresp" rid="c001">&#x2a;</xref>
<uri xlink:href="https://loop.frontiersin.org/people/587751/overview"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Huang</surname>
<given-names>Dandan</given-names>
</name>
<xref ref-type="aff" rid="aff2">
<sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Abdullah</surname>
<given-names>Borhannudin</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Luo</surname>
<given-names>Shengyao</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
<uri xlink:href="https://loop.frontiersin.org/people/1535277/overview"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Rattanakoses</surname>
<given-names>Watnawat</given-names>
</name>
<xref ref-type="aff" rid="aff3">
<sup>3</sup>
</xref>
</contrib>
</contrib-group>
<aff id="aff1">
<sup>1</sup>
<institution>Faculty of Educational Studies</institution>, <institution>Department of Sports Studies</institution>, <institution>Universiti Putra Malaysia</institution>, <addr-line>Selangor</addr-line>, <country>Malaysia</country>
</aff>
<aff id="aff2">
<sup>2</sup>
<institution>College of Physical Education</institution>, <institution>Chong Qing University</institution>, <addr-line>Chongqing</addr-line>, <country>China</country>
</aff>
<aff id="aff3">
<sup>3</sup>
<institution>Department of Khon Kaen Sport School</institution>, <institution>Thailand National Sport University</institution>, <addr-line>Bueng Nam Rak</addr-line>, <country>Thailand</country>
</aff>
<author-notes>
<fn fn-type="edited-by">
<p>
<bold>Edited by:</bold> <ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/308012/overview">Monoem Haddad</ext-link>, Qatar University, Qatar</p>
</fn>
<fn fn-type="edited-by">
<p>
<bold>Reviewed by:</bold> <ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/324542/overview">Thomas Muehlbauer</ext-link>, University of Duisburg-Essen, Germany</p>
<p>
<ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/639876/overview">Souhail Hermassi</ext-link>, Qatar University, Qatar</p>
<p>
<ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/2033346/overview">Moazzam Hussain Khan</ext-link>, Jamia Millia Islamia, India</p>
</fn>
<corresp id="c001">&#x2a;Correspondence: Nuannuan Deng, <email>GS61303@student.upm.edu.my</email>; Kim Geok Soh, <email>kims@upm.edu.my</email>
</corresp>
<fn fn-type="other">
<p>This article was submitted to Exercise Physiology, a section of the journal Frontiers in Physiology</p>
</fn>
</author-notes>
<pub-date pub-type="epub">
<day>24</day>
<month>11</month>
<year>2022</year>
</pub-date>
<pub-date pub-type="collection">
<year>2022</year>
</pub-date>
<volume>13</volume>
<elocation-id>1024418</elocation-id>
<history>
<date date-type="received">
<day>21</day>
<month>08</month>
<year>2022</year>
</date>
<date date-type="accepted">
<day>14</day>
<month>11</month>
<year>2022</year>
</date>
</history>
<permissions>
<copyright-statement>Copyright &#xa9; 2022 Deng, Soh, Huang, Abdullah, Luo and Rattanakoses.</copyright-statement>
<copyright-year>2022</copyright-year>
<copyright-holder>Deng, Soh, Huang, Abdullah, Luo and Rattanakoses</copyright-holder>
<license xlink:href="http://creativecommons.org/licenses/by/4.0/">
<p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</p>
</license>
</permissions>
<abstract>
<p>
<bold>Background:</bold> Plyometric training (PT) has been researched extensively in athletic populations. However, the effects of PT on tennis players are less clear.</p>
<p>
<bold>Methods:</bold> We aim to consolidate the existing research on the effects of PT on healthy tennis players&#x2019; skill and physical performance. On 30th May 2022, a comprehensive search of SCOPUS, PubMed, Web of Science, and SPORTDiscus (<italic>via</italic> EBSCOhost) databases was performed. PICOS was employed to define the inclusion criteria: 1) healthy tennis players; 2) a PT program; 3) compared a plyometric intervention to a control group or another exercise group, and single-group trials; 4) tested at least one measures of tennis skill or physical performance; and 5) non-randomized study trials and randomized control designs. Individual studies&#x2019; methodological quality was evaluated by using the Cochrane RoB-2 and ROBINS-I instruments. Using Grading of Recommendations Assessment, Development, and Evaluation (GRADE), the certainty of the body of evidence for each outcome was assessed, and Comprehensive Meta-Analysis software was employed for the meta-analysis.</p>
<p>
<bold>Results:</bold> Twelve studies comprising 443 tennis players aged 12.5&#x2013;25&#xa0;years were eligible for inclusion. The PT lasted from 3 to 9&#xa0;weeks. Eight studies provided data to allow for the pooling of results in a meta-analysis. A moderate positive effect was detected for PT programs on maximal serve velocity (ES &#x3d; 0.75; <italic>p</italic> &#x3c; 0.0001). In terms of measures of physical performance, small to moderate (ES &#x3d; 0.43&#x2013;0.88; <italic>p</italic> &#x3d; 0.046 to &#x3c; 0.001) effects were noted for sprint speed, lower extremity muscle power, and agility. While no significant and small effect was noted for lower extremity muscle strength (ES &#x3d; 0.30; <italic>p</italic> &#x3d; 0.115). We found no definitive evidence that PT changed other parameters (i.e., serve accuracy, upper extremity power and strength, reaction time, and aerobic endurance). Based on GRADE, the certainty of evidence across the included studies varied from very low to moderate.</p>
<p>
<bold>Conclusion:</bold> PT may improve maximal serve velocity and physical performance components (sprint speed, lower extremity muscular power, and agility) for healthy tennis players; however, more high-quality evidence about the effects of PT on the skill and physical performance of tennis players merits further investigation.</p>
<p>
<bold>Systematic Review Registration:</bold> [<ext-link ext-link-type="uri" xlink:href="https://inplasy.com/">https://inplasy.com/</ext-link>], identifier [INPLASY202250146].</p>
</abstract>
<kwd-group>
<kwd>plyometric training</kwd>
<kwd>skill</kwd>
<kwd>speed</kwd>
<kwd>power</kwd>
<kwd>muscular strength</kwd>
<kwd>agility</kwd>
</kwd-group>
</article-meta>
</front>
<body>
<sec id="s1">
<title>Introduction</title>
<p>In racket sports such as tennis, technical and tactical skills are crucial for players to reach the top ranks and are primarily related to their performance levels (<xref ref-type="bibr" rid="B100">Strecker et al., 2011</xref>; <xref ref-type="bibr" rid="B66">MacCurdy, 2018</xref>). Tennis technical skills are most noticeable in serves and groundstrokes (<xref ref-type="bibr" rid="B41">Gillet et al., 2009</xref>; <xref ref-type="bibr" rid="B108">Ulbricht et al., 2016</xref>; <xref ref-type="bibr" rid="B31">Fauzi et al., 2021</xref>). Tactical skills refer to the knowledge of on-court decision-making activities and in-game adaptations (<xref ref-type="bibr" rid="B25">Elferink-Gemser et al., 2010</xref>). According to a systematic review, tennis players&#x2019; technical and tactical abilities are positively associated with their competition performance levels (<xref ref-type="bibr" rid="B56">Kolman et al., 2019</xref>). On the other hand, to be successful in tennis, cardiovascular fitness, sprint rehearsal ability, change of direction speed, muscle strength and muscle power are among the required physical components (<xref ref-type="bibr" rid="B32">Fernandez-Fernandez et al., 2009</xref>; <xref ref-type="bibr" rid="B75">Pereira et al., 2016</xref>; <xref ref-type="bibr" rid="B9">Bj&#xf6;rklund et al., 2020</xref>). The stronger the physical foundation, the more opportunities for developing technical, tactical, and psychological skills (<xref ref-type="bibr" rid="B11">Bompa and Haff, 2009</xref>; <xref ref-type="bibr" rid="B17">Chunlei, 2016</xref>; <xref ref-type="bibr" rid="B64">Lambrich and Muehlbauer, 2022</xref>), as well as preventing injuries (<xref ref-type="bibr" rid="B14">Carter and Micheli, 2011</xref>). Furthermore, it is well acknowledged that players need enhanced levels of physical fitness to execute advanced strokes and compete successfully against more advanced opponents (<xref ref-type="bibr" rid="B108">Ulbricht et al., 2016</xref>). Therefore, players require good technical and tactical skills, along with excellent physical performance to succeed in tennis. In this regard, it becomes very important to use training methods specific to the necessities of tennis.</p>
<p>Fortunately, several types of exercise interventions can enhance performance in tennis players. For instance, resistance training can increase serve velocity (<xref ref-type="bibr" rid="B61">Kraemer et al., 2003</xref>), while, strength training can enhance forehand and backhand hitting speed (<xref ref-type="bibr" rid="B103">Terraza-Rebollo et al., 2017</xref>). Moreover, core training, balance training and sprint training are known to improve the speed and strength of tennis players (<xref ref-type="bibr" rid="B93">Sannicandro et al., 2014</xref>; <xref ref-type="bibr" rid="B7">Bashir et al., 2019</xref>; <xref ref-type="bibr" rid="B71">Moya-Ramon et al., 2020</xref>). High-intensity interval training improves young tennis athletes&#x2019; aerobic performance (<xref ref-type="bibr" rid="B36">Fernandez-Fernandez et al., 2017</xref>). Notably, among the many kinds of exercises, plyometrics assist to develop power, a foundation from which the athlete can refine the skills of their sport (<xref ref-type="bibr" rid="B20">Davies et al., 2015</xref>).</p>
<p>P<italic>lyometric training</italic> involves a stretch-shortening cycle (SSC) that comprises a lengthening action (eccentric movement), followed by a shortening action (concentric movement) (<xref ref-type="bibr" rid="B18">Clark et al., 2016</xref>). The mechanism underlying PT mainly involves two parts. The first part transforms the elastic energy stored during muscle stretching into the power output of concentric contraction (<xref ref-type="bibr" rid="B111">Wilk et al., 1993</xref>). The second part applies proprioceptor signals induced during muscle stretching to detect muscle tension and length (stretch reflex) (<xref ref-type="bibr" rid="B6">Bal et al., 2012</xref>). The sensory signal then sends nerve impulses into the spinal cord to transfer information to alpha motor neurons that activate agonist muscles, recruit motor units, and suppress the contraction of antagonist muscles (<xref ref-type="bibr" rid="B76">Potach, 2004</xref>). Meanwhile, the SSC is a model that explains the energy-storing capacities of the series elastic component and the activation of the stretch reflex, which allow for a maximal increase in muscle recruitment in the shortest time (<xref ref-type="bibr" rid="B76">Potach, 2004</xref>). There are three distinct phases to the SSC: Prior to muscular activation, phase I, the eccentric phase, increases muscle spindle activity by pre-stretching the muscle; the muscle&#x2019;s elastic characteristics store the potential energy created during the loading period (<xref ref-type="bibr" rid="B76">Potach, 2004</xref>; <xref ref-type="bibr" rid="B20">Davies et al., 2015</xref>). Phase II, the amortization phase, is the interval between the conclusion of the eccentric contraction and the beginning of the concentric contraction; during this phase, dynamic stabilization occurs (<xref ref-type="bibr" rid="B16">Cavagna, 1977</xref>). Rapid transition from an eccentric contraction to a concentric contraction elicits a strong reaction (<xref ref-type="bibr" rid="B18">Clark et al., 2016</xref>). Phase III, the concentric phase, consists of a concentric contraction that improves muscular function after the eccentric phase (<xref ref-type="bibr" rid="B111">Wilk et al., 1993</xref>; <xref ref-type="bibr" rid="B18">Clark et al., 2016</xref>).</p>
<p>Based on the physiological principles mentioned above, PT optimizes the SSC and related neuro-mechanical mechanisms (<xref ref-type="bibr" rid="B68">Markovic and Mikulic, 2010</xref>) has the potential and training advantage in improving sports performance in athletic populations. Previous studies have proven PT&#x2019;s effects on skill performance, and the results are extremely positive. For instance, <xref ref-type="bibr" rid="B57">Komal and Singh. (2013)</xref> indicates that PT of 8&#xa0;weeks has a significant effect on the dribbling and speed shot performance of basketball players. <xref ref-type="bibr" rid="B45">Hall et al. (2016)</xref> found that the implementation of a 6-week PT program can contribute to improving the handspring vault performance of competitive gymnasts. The study by <xref ref-type="bibr" rid="B90">Rubley et al. (2011)</xref> found an improvement in the kicking performance of soccer players after a 14-week PT program. At the same time, PT has been shown to be effective in improving athletes&#x2019; physical performance (e.g., sprint, jump, muscle strength, balance, endurance, agility, and flexibility) regardless of age, gender, training experience, and competition level (<xref ref-type="bibr" rid="B1">Agostini et al., 2017</xref>; <xref ref-type="bibr" rid="B10">Bogdanis et al., 2019</xref>; <xref ref-type="bibr" rid="B30">Fathi et al., 2019</xref>; <xref ref-type="bibr" rid="B65">Li et al., 2019</xref>; <xref ref-type="bibr" rid="B12">Bouteraa et al., 2020</xref>; <xref ref-type="bibr" rid="B54">Jlid et al., 2020</xref>; <xref ref-type="bibr" rid="B101">Tammam and Hashem, 2020</xref>; <xref ref-type="bibr" rid="B2">Ahmadi et al., 2021</xref>; <xref ref-type="bibr" rid="B88">Rojano Ortega et al., 2021</xref>; <xref ref-type="bibr" rid="B89">Romero et al., 2021</xref>; <xref ref-type="bibr" rid="B91">S&#xe1;ez De Villarreal et al., 2021</xref>; <xref ref-type="bibr" rid="B55">Kim et al., 2022</xref>; <xref ref-type="bibr" rid="B59">Kosova et al., 2022</xref>). Moreover, PT has also been used to benefit the prevention of ankle injuries (<xref ref-type="bibr" rid="B53">Huang et al., 2021</xref>). Thus, we formulated two hypotheses based on previous work. First, we hypothesized that PT would improve skill performance in tennis players. Second, we hypothesized PT would exert beneficial effects on physical performance among tennis players.</p>
<p>In recent years, several reviews and meta-analyses on the impact of PT on athletic performance characteristics have been published (<xref ref-type="bibr" rid="B95">Silva et al., 2019</xref>; <xref ref-type="bibr" rid="B81">Ramirez-Campillo et al., 2020a</xref>; <xref ref-type="bibr" rid="B82">2020b</xref>; <xref ref-type="bibr" rid="B84">2021a</xref>; <xref ref-type="bibr" rid="B83">2021b</xref>). However, these meta-analyses included athletes from various sports (i.e., volleyball players, soccer players, basketball players). Because the effects of PT may differ based on the athlete&#x2019;s athletic history, the results of these studies cannot be applied directly to tennis players (<xref ref-type="bibr" rid="B83">Ramirez-Campillo et al., 2021b</xref>; <xref ref-type="bibr" rid="B99">Sole et al., 2021</xref>). According to our knowledge, no previous systematic reviews and meta-analyses have been undertaken on the effects of PT on tennis players, exposing a gap in the literature. Implementing a systematic review and meta-analysis may assist in identifying gaps and limitations in the PT literature and provide practitioners and researchers in adjacent domains with vital information regarding potential future research routes (<xref ref-type="bibr" rid="B85">Ramirez-Campillo et al., 2022</xref>). Increasingly, experimental research has investigated the influence of PT on tennis players. For example, comparing PT to conventional tennis exercises, <xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al. (2016)</xref> found that PT appears to be an acceptable stimulus for enhancing physical qualities and serve velocity in tennis players. However, this evidence has not yet been gathered comprehensively. Consequently, a systematic review and meta-analysis are required to examine the effects of PT on tennis performance.</p>
<p>Therefore, this systematic review and meta-analysis serves a dual function. The initial purpose of this review was to summarize the present status of the literature on this topic, noting deficiencies in existing research and outlining the path that future researchers should take to address these deficiencies. The second objective was to finally quantify the effects of PT on healthy tennis players in order to determine the influence of this type of training on skill and physical performance, extending the existing understanding of the effects of PT on athletes and widening tennis-specific training methods.</p>
</sec>
<sec sec-type="methods" id="s2">
<title>Methods</title>
<p>There was adherence to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines throughout the entire process of conducting this systematic review (<xref ref-type="bibr" rid="B74">Page et al., 2021</xref>), and the review protocol has been registered on <ext-link ext-link-type="uri" xlink:href="http://Inplasy.com">Inplasy.com</ext-link> (INPLASY202250146).</p>
<sec id="s2-1">
<title>Search strategy</title>
<p>We systematically searched SCOPUS, PubMed, Web of Science, and SPORTDiscus (<italic>via</italic> EBSCOhost) electronic databases from inception until 30th May 2022. A systematic investigation of the topic was carried out utilizing the Boolean operations AND and OR. For keyword selection and search strategy development, the authors sought advice from experienced librarians. The keywords are as follows: (&#x201c;plyometric training&#x201d; OR &#x201c;plyometric exercise&#x2a;&#x201d; OR &#x201c;plyometric drill&#x2a;&#x201d; OR &#x201c;plyometr&#x2a;&#x201d; OR &#x201c;ballistic six&#x201d; OR &#x201c;ballistic training&#x201d; OR &#x201c;explosive&#x201d; OR &#x201c;force-velocity&#x201d; OR &#x201c;stretch-shortening cycle&#x201d; OR &#x201c;stretch-shortening exercise&#x201d; OR &#x201c;complex training&#x201d; OR &#x201c;jump training&#x201d;) AND (&#x201c;tennis&#x201d; OR &#x201c;tennis player&#x2a;&#x201d; OR &#x201c;tennis athlete&#x2a;&#x201d;). The main databases search string is provided in <xref ref-type="sec" rid="s12">Supplementary Appendix S1</xref>. Furthermore, to find additional literature that might not have shown up in the search results using the four databases, a search was also carried out on Google Scholar and based on the reference lists of selected papers, previously relevant reviews and meta-analyses (<xref ref-type="bibr" rid="B96">Singla et al., 2018</xref>; <xref ref-type="bibr" rid="B99">Sole et al., 2021</xref>; <xref ref-type="bibr" rid="B19">Colomar et al., 2022</xref>; <xref ref-type="bibr" rid="B112">Xiao et al., 2022</xref>).</p>
</sec>
<sec id="s2-2">
<title>Study selection</title>
<p>Firstly, two authors (ND, DH) uploaded the collected literature information to the Endnote X9 reference management software based on title, type, authors, and year of publication. After that, all duplicates were removed (ND). Two independent authors (ND, KS) screened each study&#x2019;s title and abstract for potentially relevant full-text literature. After that, the same authors compared the entire study text to the inclusion and exclusion criteria and chose publications that fulfilled the requirements. Two independent authors (ND, KS) worked out their disagreements through discussion; if they disagreed, a third author (BA) was consulted until they reached an agreement. <xref ref-type="fig" rid="F1">Figure 1</xref> illustrates the details of the selection procedure.</p>
<fig id="F1" position="float">
<label>FIGURE 1</label>
<caption>
<p>PRISMA flow diagram.</p>
</caption>
<graphic xlink:href="fphys-13-1024418-g001.tif"/>
</fig>
</sec>
<sec id="s2-3">
<title>Eligibility criteria</title>
<p>To evaluate whether studies could be included in the review, we employed the PICOS approach (<xref ref-type="table" rid="T1">Table 1</xref>). The selection criteria that we considered for our systematic review are stated below:<list list-type="simple">
<list-item>
<p>1) Healthy tennis players, with no limitations on their gender, age or level.</p>
</list-item>
<list-item>
<p>2) Experimental studies included a PT program to determine tennis players&#x2019; skill and/or physical performance; studies using multicomponent training with plyometric components (e.g., neuromuscular training) or combinations of PT with other types of exercise training were also included.</p>
</list-item>
<list-item>
<p>3) Compare a PT intervention to a control or exercise group, and single-group trials were also included. Additionally, studies involving a meta-analysis need to have the mean and standard deviation for the experimental and control groups at pre-and post-test.</p>
</list-item>
<list-item>
<p>4) Reported one or more of the following outcomes: skill performance (e.g., maximal stroke velocity, stroke accuracy) and physical performance (e.g., jump height, sprint speed, agility).</p>
</list-item>
<list-item>
<p>5) To avoid the exclusion of potentially pertinent high-quality research, we took the inclusion of full-text, peer-reviewed, original RCTs and non-RCTs into consideration. Our study focused only on English-language articles, since it may be difficult to translate articles written in different languages, and previous research has demonstrated that nearly all of the literature (99.6%) on plyometric jump training was in English (<xref ref-type="bibr" rid="B80">Ramirez-Campillo et al., 2018</xref>).</p>
</list-item>
</list>
</p>
<table-wrap id="T1" position="float">
<label>TABLE 1</label>
<caption>
<p>Eligibility criteria according to the PICOS conditions.</p>
</caption>
<table>
<thead valign="top">
<tr>
<th align="left">Category</th>
<th align="left">Inclusion criteria</th>
<th align="left">Exclusion criteria</th>
</tr>
</thead>
<tbody valign="top">
<tr>
<td align="left">Population</td>
<td align="left">Healthy tennis players</td>
<td align="left">Tennis players with health problems (e.g., injuries, recent surgery)</td>
</tr>
<tr>
<td align="left">Intervention</td>
<td align="left">A plyometric training program, defined as upper-body plyometrics (medicine ball exercises, push-ups, and chess press) or lower-body plyometrics (unilateral or bilateral bounds, jumps, hops, and/or skips) or combined upper and lower-body plyometrics that commonly utilize a pre-stretch or countermovement stressing the stretch-shortening cycle</td>
<td align="left">Exercise interventions not involving plyometric training or exercise interventions involving plyometric training programs representing less than 50% of the total training load (i.e., volume, e.g., number of exercises) when delivered in conjunction with other training interventions (e.g., sprint training)</td>
</tr>
<tr>
<td align="left">Comparator</td>
<td align="left">Two or more groups and single-group trials</td>
<td align="left">Not under supervision</td>
</tr>
<tr>
<td align="left">Outcome</td>
<td align="left">At least one measure related to tennis skill (e.g., maximal stroke velocity, stroke accuracy) or physical performance (e.g., sprint speed, jump height, agility)</td>
<td align="left">Lack of baseline and/or follow-up data</td>
</tr>
<tr>
<td align="left">Study design</td>
<td align="left">RCT or non-RCT</td>
<td align="left">Cross-sectional studies, case studies, articles not written in English</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<fn>
<p>RCT, Randomized control trial; Non-RCT, Non-Randomized control trial.</p>
</fn>
</table-wrap-foot>
</table-wrap>
<p>The following exclusion criteria were used in our systematic review:<list list-type="simple">
<list-item>
<p>1) Unhealthy tennis players or those with injuries.</p>
</list-item>
<list-item>
<p>2) Training programs not involving PT or training interventions with plyometric exercises representing less than half of the training volume when delivered with other training interventions (e.g., sprint training).</p>
</list-item>
<list-item>
<p>3) Cross-sectional, review papers and training-related works that did not focus on the impact of PT were excluded.</p>
</list-item>
<list-item>
<p>4) Excluding case reports, brief communications, letters to the editor, invited comments, errata, overtraining studies, patents, and retrospective, papers for which only the abstract was available.</p>
</list-item>
</list>
</p>
</sec>
<sec id="s2-4">
<title>Data extraction</title>
<p>Two reviewers (ND, DH) retrieved data from each study using a Microsoft Excel spreadsheet (Microsoft Corporation, Redmond, WA, United States), and a third reviewer (KS) validated the data. The data considered were: 1) Name of the first author and year of publication; 2) Subject characteristics: sample size, gender, age (years), and tennis experience; and 3) Characteristics of the PT intervention, which are training protocol, training frequency (days/week), duration (weeks), intensity level (e.g., maximal), rest time between sessions (hours), rest time between sets (s), rest time between repetitions (s), type of progressive PT overload (e.g., volume-based; technique-based), training period (e.g., in-season), PT replaced (if applicable) a component of the regular tennis practice.</p>
</sec>
<sec id="s2-5">
<title>Risk of bias in individual studies and certainty of evidence</title>
<p>According to the guideline on the webpage for Cochrane Training, two reviewers (ND, KS) independently assessed the risk of bias of each of the identified RCTs using the updated Cochrane risk of bias assessment for randomized trials (RoB-2) (<xref ref-type="bibr" rid="B50">Higgins et al., 2022</xref>). Risk Of Bias In Non-randomized Research of Interventions (ROBINS-I) was used to evaluate the risk of bias in non-randomized controlled trials (<xref ref-type="bibr" rid="B50">Higgins et al., 2022</xref>). GRADE was used to analyze and summarize the confidence of evidence following the GRADE handbook&#x2019;s principles (<xref ref-type="bibr" rid="B94">Sch&#xfc;nemann et al., 2020</xref>). First, we categorized all relevant trials based on the outcomes they reported. Then, to determine the confidence of evidence, the following six factors were considered: research design, study limitations, inconsistency, indirectness, imprecision, and publication bias. Outcomes were assessed separately for RCTs and non-RCTs. Two individuals authored this work (ND, KS).</p>
</sec>
<sec id="s2-6">
<title>Data synthesis and meta-analysis</title>
<p>If three or more relatively homogeneous studies supplied explicit pre-and post-test data for the control and experimental groups using the same parameters, these papers were combined for meta-analysis (<xref ref-type="bibr" rid="B83">Ramirez-Campillo et al., 2021b</xref>; <xref ref-type="bibr" rid="B115">Ramachandran et al., 2021</xref>). In contrast, a narrative synthesis of the findings is conducted (<xref ref-type="bibr" rid="B26">Elgueta-Cancino et al., 2022</xref>). On the basis of pre- and post-intervention performance means and standard deviations, between-group effect sizes (ES; Hedge&#x2019;s g) were computed (SD). The data were standardized using the post-score SD. The inverse-variance random-effects model was utilized for meta-analyses because it assigns a proportional weight to trials depending on the magnitude of their individual standard errors (<xref ref-type="bibr" rid="B23">Deeks et al., 2008</xref>) and aids analysis while accounting for heterogeneity across studies (<xref ref-type="bibr" rid="B58">Kontopantelis et al., 2013</xref>). If the needed information was not available in the original article or additional materials, the authors were contacted. If contact was not returned or data was unavailable, the study was excluded from the meta-analysis. The ES values were presented with 95% confidence intervals (95% CIs). The ES magnitudes were interpreted using the following scale: &#x3c; 0.2, trivial; 0.2&#x2013;0.6, small; &#x3e; 0.6&#x2013;1.2, moderate; &#x3e;1.2&#x2013;2.0, large; &#x3e;2.0&#x2013;4.0, very large; &#x3e;4.0, extremely large (<xref ref-type="bibr" rid="B51">Hopkins et al., 2009</xref>). In some investigations including multiple PT groups, the control group was proportionally divided to permit comparisons across all participants (<xref ref-type="bibr" rid="B49">Higgins et al., 2008</xref>). To assess heterogeneity, the I<sup>2</sup> statistic was employed. Low, moderate, and high degrees of heterogeneity, were determined to be 25%, 25&#x2013;75%, and &#x3e;75%, respectively (<xref ref-type="bibr" rid="B48">Higgins et al., 2003</xref>). The risk of publication bias across studies was assessed using the extended Egger&#x2019;s test (<xref ref-type="bibr" rid="B24">Egger et al., 1997</xref>). Furthermore, a sensitivity analysis was performed when Egger&#x2019;s test was significant (<italic>p</italic> &#x3c; 0.05). An analysis of all available data was carried out using Comprehensive Meta-Analysis software (version three; Biostat, Englewood, NJ, United States).</p>
</sec>
</sec>
<sec sec-type="results" id="s3">
<title>Results</title>
<sec id="s3-1">
<title>Study selection</title>
<p>The database search produced 361 articles, with an additional five articles discovered <italic>via</italic> Google Scholar and reference lists. In total, 224 research articles remained after duplicates were removed, and the remaining articles were eliminated based on title and abstract screening. An evaluation of the remaining 111 studies was conducted independently by two researchers. The systematic review included twelve studies after the final screening process (<xref ref-type="table" rid="T2">Table 2</xref>), and eight articles were included in the meta-analysis (<xref ref-type="sec" rid="s12">Supplementary Appendix S2</xref>). The detailed selection procedure for the studies is shown in <xref ref-type="fig" rid="F1">Figure 1</xref>.</p>
<table-wrap id="T2" position="float">
<label>TABLE 2</label>
<caption>
<p>Characteristics of the studies examined in the present review.</p>
</caption>
<table>
<thead valign="top">
<tr>
<th rowspan="2" align="left">Study</th>
<th rowspan="2" align="left">Design</th>
<th colspan="4" align="left">Population characteristics</th>
<th rowspan="2" align="left">Interventions</th>
<th rowspan="2" align="left">Comparator</th>
<th colspan="2" align="left">Measures index</th>
<th colspan="2" align="left">Outcomes</th>
</tr>
<tr>
<th align="left">N</th>
<th align="left">Sex</th>
<th align="left">Age</th>
<th align="left">Exp/level</th>
<th align="left">Skill</th>
<th align="left">Physical</th>
<th align="left">Skill</th>
<th align="left">Physical</th>
</tr>
</thead>
<tbody valign="top">
<tr>
<td align="left">
<xref ref-type="bibr" rid="B92">Salonikidis and Zafeiridis (2008)</xref>
</td>
<td align="left">Pre-post test</td>
<td align="left">64</td>
<td align="left">M</td>
<td align="left">21.1 &#xb1; 1.3&#xa0;yrs</td>
<td align="left">2&#x2013;3&#xa0;yrs novice</td>
<td align="left">Freq: 3&#xa0;times/week Time: NR Length: 9&#xa0;weeks</td>
<td align="left">Plyometric training (EG1), Plyometric <bold>&#x2b;</bold> tennis regular drills (EG2), Control group (CG)</td>
<td align="left"/>
<td rowspan="2" align="left">Reaction time (RTSS); Sprint (4&#xa0;mSS, 4&#xa0;mFS, 12&#xa0;mFS, 12&#xa0;mFST); Strength (L: Fmax); Power (L: DJ)</td>
<td align="left"/>
<td rowspan="2" align="left">EG1:RTSS&#x2191;, 4mSS&#x2191;, 4mFS&#x2191;, 12mFS&#x2194;, 12mFST&#x2194;, DJ&#x2191;, Fmax&#x2191;; EG2: RT&#x2191;, 4mSS&#x2191;, 4mFS&#x2191;, 12mFS&#x2191;, 12mFST&#x2191;, DJ&#x2191;, Fmax&#x2191;</td>
</tr>
<tr>
<td align="left">
<xref ref-type="bibr" rid="B40">Gelen et al. (2012)</xref>
</td>
<td align="left">within-subject, repeated-measures</td>
<td align="left">26</td>
<td align="left">NR</td>
<td align="left">15.1 &#xb1; 4.2&#xa0;yrs</td>
<td align="left">8.4 &#xb1; 3.8&#xa0;yrs elite</td>
<td align="left">Time: 34&#xa0;min</td>
<td align="left">Static stretching (EG1), Dynamic exercises (EG2), Plyometric exercises (EG3), Control group (CG)</td>
<td align="left">Serve (SV)</td>
<td align="left">SV&#x2191;</td>
</tr>
<tr>
<td align="left">
<xref ref-type="bibr" rid="B8">Behringer et al. (2013)</xref>
</td>
<td align="left">Pre-post test</td>
<td align="left">36</td>
<td align="left">M</td>
<td align="left">15.03 &#xb1; 1.64&#xa0;yrs</td>
<td align="left">averaged 6.15&#xa0;yrs local tennis club</td>
<td align="left">Freq: 2&#xa0;times/week Time: 45&#xa0;min Length: 8&#xa0;weeks</td>
<td align="left">Resistance training (EG1), Plyometric training (EG2), Control group (CG)</td>
<td align="left">Serve (SV, SA)</td>
<td align="left">Strength (10RM test: U: chest press, pull-down machine, abdominal press; L: leg press)</td>
<td align="left">SV&#x2191;, SA&#x2194;</td>
<td align="left">10RM test&#x2191;</td>
</tr>
<tr>
<td align="left">
<xref ref-type="bibr" rid="B72">&#xd6;l&#xe7;&#xfc;c&#xfc; et al. (2013)</xref>
</td>
<td align="left">Pre-post test</td>
<td align="left">40</td>
<td align="left">M</td>
<td align="left">20&#x2013;25&#xa0;yrs</td>
<td align="left">NR tournament</td>
<td align="left">Freq: 2&#xa0;times/week Time: 35&#xa0;min Length: 8&#xa0;weeks</td>
<td align="left">Plyometric training (EG), Control group (CG)</td>
<td rowspan="2" align="left">Serve (SV)</td>
<td align="left">Strength (isokinetic test, U: shoulder joint; L: knee joint)</td>
<td rowspan="2" align="left">SV&#x2191;</td>
<td align="left">All isokinetic tests &#x2191;</td>
</tr>
<tr>
<td align="left">
<xref ref-type="bibr" rid="B34">Fernandez-Fernandez et al. (2015)</xref>
</td>
<td align="left">Pre-post test</td>
<td align="left">16</td>
<td align="left">M</td>
<td align="left">16.9 &#xb1; 0.5&#xa0;yrs</td>
<td align="left">8.0 &#xb1; 2.6&#xa0;yrs elite</td>
<td align="left">Freq: 2&#xa0;times/week Time: 30&#x2013;60&#xa0;min Length: 8&#xa0;weeks</td>
<td align="left">Explosive strength and repeated sprint training (EG), Control group (CG)</td>
<td align="left">Sprint (10&#xa0;m, 20&#xa0;m, 30&#xa0;m, RSAb, RSAm); Power (L: CMJ); Aerobic endurance (VIFT)</td>
<td align="left">10&#xa0;m&#x2191;, CMJ&#x2191;, RSAb&#x2191;, RSAm&#x2191;, 20&#xa0;m&#x2194;, 30&#xa0;m&#x2194;, VIFT&#x2194;</td>
</tr>
<tr>
<td align="left">
<xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al. (2016)</xref>
</td>
<td align="left">Pre-post test</td>
<td align="left">60</td>
<td align="left">M</td>
<td align="left">12.5 &#xb1; 0.3&#xa0;yrs</td>
<td align="left">&#x3e;3&#xa0;yrs international tennis academy</td>
<td align="left">Freq: 2&#xa0;times/week Time: 30&#x2013;60&#xa0;min Length: 8&#xa0;weeks</td>
<td align="left">Plyometric training (EG), Control group (CG)</td>
<td rowspan="2" align="left">Serve (SV, SA)</td>
<td align="left">Sprint (5&#xa0;m,10&#xa0;m,20&#xa0;m); Power (U: MBT; L: CMJ, SLJ); Agility (5-0&#x2013;5 test)</td>
<td rowspan="2" align="left">SV&#x2191;, SA&#x2191;</td>
<td align="left">5&#xa0;m&#x2191;, 10&#xa0;m&#x2191;, 20&#xa0;m&#x2191;, 5-0&#x2013;5 test&#x2191;, SLJ&#x2191;, MBT&#x2191;, CMJ&#x2191;</td>
</tr>
<tr>
<td align="left">
<xref ref-type="bibr" rid="B86">Rathore (2016)</xref>
</td>
<td align="left">Pre-post test</td>
<td align="left">60</td>
<td align="left">M</td>
<td align="left">18&#x2013;23&#xa0;yrs</td>
<td align="left">NR professional</td>
<td align="left">Freq: 3&#xa0;times/week Time: 45&#xa0;min Length: 8&#xa0;weeks</td>
<td align="left">Plyometric training (EG1), Resistance training (EG2), Control group (CG)</td>
<td align="left">Agility (Illinois test)</td>
<td align="left">Illinois test&#x2191;</td>
</tr>
<tr>
<td align="left">
<xref ref-type="bibr" rid="B37">Fernandez-Fernandez et al. (2018)</xref>
</td>
<td align="left">Pre-post test</td>
<td align="left">16</td>
<td align="left">M</td>
<td align="left">12.9 &#xb1; 0.4&#xa0;yrs</td>
<td align="left">3.0 &#xb1; 1.2&#xa0;yrs elite</td>
<td align="left">Freq: 2&#xa0;times/week Time: 20&#x2013;40&#xa0;min Length: 8&#xa0;weeks</td>
<td align="left">NMT before tennis-specific training (EG1), NMT after tennis-specific training (EG2)</td>
<td rowspan="5" align="left">Serve (SV)</td>
<td align="left">Sprint (5, 10, and 20&#xa0;m); Power (U: MBT; L: CMJ); Agility (5-0-5 test)</td>
<td rowspan="5" align="left">SV&#x2192;</td>
<td align="left">EG1: 5&#xa0;m&#x2191;, 10&#xa0;m&#x2191;, 20&#xa0;m&#x2191;, 5-0-5test&#x2191;, CMJ&#x2191;, MBT&#x2191;, SV&#x2191;; EG2: 10&#xa0;m&#x2191;, 20&#xa0;m&#x2193;, 5&#xa0;m&#x2193;, 5&#x2013;05-test&#x2193;, MBT&#x2194;, CMJ&#x2193;</td>
</tr>
<tr>
<td align="left">
<xref ref-type="bibr" rid="B63">Lakshmikanth et al. (2018)</xref>
</td>
<td align="left">Pre-post test</td>
<td align="left">30</td>
<td align="left">Mixed</td>
<td align="left">18&#x2013;22&#xa0;yrs</td>
<td align="left">NR college students</td>
<td align="left">Freq: 1&#xa0;times/week Time: NR Length: 6&#xa0;weeks</td>
<td align="left">Plyometric training (EG1), Control group (CG)</td>
<td align="left">Agility (Illinois test, tennis-specific agility test)</td>
<td align="left">Illinois test&#x2191;, tennis-specific agility test&#x2191;</td>
</tr>
<tr>
<td align="left">
<xref ref-type="bibr" rid="B114">Ziagkas et al. (2019)</xref>
</td>
<td align="left">Pre-post test</td>
<td align="left">24</td>
<td align="left">M</td>
<td align="left">20.9 &#xb1; 0.66&#xa0;yrs</td>
<td align="left">1&#x2013;3&#xa0;yrs amateur</td>
<td align="left">Freq: 2&#xa0;times/week Time:30&#x2013;60&#xa0;min Length:8&#xa0;weeks</td>
<td align="left">Plyometric training (EG), Control group (CG)</td>
<td align="left">Agility (Hexagon Test, Spider Test)</td>
<td align="left">Hexagon Test&#x2191;, Spider Test&#x2191;</td>
</tr>
<tr>
<td align="left">
<xref ref-type="bibr" rid="B70">Mohanta et al. (2019)</xref>
</td>
<td align="left">Pre-post test</td>
<td align="left">40</td>
<td align="left">M</td>
<td align="left">18&#x2013;25&#xa0;yrs</td>
<td align="left">&#x3e;2&#xa0;yrs local tennis academy</td>
<td align="left">Freq: 2&#xa0;times/week Time: 30&#x2013;60&#xa0;min Length: 8&#xa0;weeks</td>
<td align="left">Plyometric training (EG1), Circuit training (EG2)</td>
<td align="left">Sprint (50-meter dash test); Strength (U:1RM chest press test); Power (L: VJ); Agility (T-test)</td>
<td align="left">50-meter dash test&#x2191;, 1RM chest press test&#x2191;, VJ&#x2191;, T-test&#x2191;</td>
</tr>
<tr>
<td align="left">
<xref ref-type="bibr" rid="B52">Hotwani et al. (2021)</xref>
</td>
<td align="left">Pre-post test</td>
<td align="left">31</td>
<td align="left">Mixed</td>
<td align="left">15&#x2013;18&#xa0;yrs</td>
<td align="left">NR local tennis academy</td>
<td align="left">Freq: NR Time: 60&#xa0;min Length: 3&#xa0;weeks</td>
<td align="left">Plyometrics &#x2b; sprint training (EG)</td>
<td align="left">Agility (T-test, Illinois Test)</td>
<td align="left">T-test&#x2191;, Illinois Test&#x2191;</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<fn>
<p>NR, Not reported; yrs, Years; Exp, Tennis experience; M, Male; F, Female; Freq, Frequency; CG, Control group; EG, Experimental group; NMT, Neuromuscular training; CMJ, Vertical countermovement jump; SLJ, Standing long jump; 505 Test, modified 505 agility test; DJ, drop jump; VJ, Vertical jump; MBT, Medicine ball throw; SV, Serve velocity; SA, Serve accuracy; RTSS, Reaction time single step; 4mSS, 4-m side-step; 4mFS, 4-m forward sprint; 12&#xa0;mFS, 12-m forward sprint; 12&#xa0;mFST, 12-m forward sprint with turn; RSAb, Repeated sprint ability best; RSAm, Repeated sprint ability mean; 10RM, 10 Repetition Maximum Testing; VIFT, Velocity of the intermittent fitness test; 1RM, 1 Repetition maximum; 10RM; 10 Repetition maximum; Fmax, Maximum isometric force (leg); U, Upper extremity; L, Lower extremity; &#x2191;, significant within-group improvement; &#x2194;, non-significant within-group.</p>
</fn>
</table-wrap-foot>
</table-wrap>
</sec>
<sec id="s3-2">
<title>Risk of bias in individual studies and certainty of evidence</title>
<p>RoB-2 was applied to five RCTs, while ROBINS-I was applied to seven non-RCTs. Ten trials were found to have an overall moderate risk of bias or presented some concerns, only two papers showed a lower risk of bias (see <xref ref-type="fig" rid="F2">Figure 2</xref>). <xref ref-type="fig" rid="F2">Figure 2A</xref> depicts the findings of the RoB-2 evaluations. Only one research described the method for generating randomization sequences utilizing stratified block randomization and specifically documented allocation concealment (<xref ref-type="bibr" rid="B8">Behringer et al., 2013</xref>), whereas four articles did not completely describe the randomized procedure (<xref ref-type="bibr" rid="B92">Salonikidis and Zafeiridis, 2008</xref>; <xref ref-type="bibr" rid="B40">Gelen et al., 2012</xref>; <xref ref-type="bibr" rid="B86">Rathore, 2016</xref>; <xref ref-type="bibr" rid="B114">Ziagkas et al., 2019</xref>). Only two studies had preregistered protocols (<xref ref-type="bibr" rid="B92">Salonikidis and Zafeiridis, 2008</xref>; <xref ref-type="bibr" rid="B8">Behringer et al., 2013</xref>), and three of the studies therefore had some concerns regarding bias in the selection of the reported results (<xref ref-type="bibr" rid="B40">Gelen et al., 2012</xref>; <xref ref-type="bibr" rid="B86">Rathore, 2016</xref>; <xref ref-type="bibr" rid="B114">Ziagkas et al., 2019</xref>). <xref ref-type="fig" rid="F2">Figure 2B</xref> depicts a graphical representation of the outcomes of ROBINS-I evaluations. One of the non-RCTs discovered some concerns due to missing data, owing to an approximate 15% dropout rate (<xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al., 2016</xref>), two studies had moderate risk of bias in domain of deviations from intended intervention (<xref ref-type="bibr" rid="B72">&#xd6;l&#xe7;&#xfc;c&#xfc;, 2013</xref>; <xref ref-type="bibr" rid="B63">Lakshmikanth et al., 2018</xref>), and three studies had moderate risk regarding bias in the selection of the reported results (<xref ref-type="bibr" rid="B72">&#xd6;l&#xe7;&#xfc;c&#xfc; et al., 2013</xref>; <xref ref-type="bibr" rid="B37">Fernandez-Fernandez et al., 2018</xref>; <xref ref-type="bibr" rid="B52">Hotwani et al., 2021</xref>).</p>
<fig id="F2" position="float">
<label>FIGURE 2</label>
<caption>
<p>Risk of bias. <bold>(A)</bold> Results for RCTs, <bold>(B)</bold> results for non-RCTs. &#x2a;Created using Robvis (visualization tool): <xref ref-type="bibr" rid="B69">McGuinness and Higgins (2021)</xref>.</p>
</caption>
<graphic xlink:href="fphys-13-1024418-g002.tif"/>
</fig>
<p>According to the GRADE assessment (<xref ref-type="sec" rid="s12">Supplementary Appendix S3</xref>), in terms of RCTs, the certainty of evidence is considered very lower to moderate. In terms of non-RCTs, the certainty of evidence is considered very lower to low.</p>
</sec>
<sec id="s3-3">
<title>Study characteristics</title>
<p>
<xref ref-type="table" rid="T2">Table 2</xref> summarizes the study and intervention characteristics. Moreover, the training protocol of each study can be found in <xref ref-type="sec" rid="s12">Supplementary Appendix S4</xref>. The twelve included papers were carried out between 2008 and 2021, with 443 tennis players. Across the studies, 1) Gender: nine studies focused on men (<xref ref-type="bibr" rid="B92">Salonikidis and Zafeiridis, 2008</xref>; <xref ref-type="bibr" rid="B8">Behringer et al., 2013</xref>; <xref ref-type="bibr" rid="B34">Fernandez-Fernandez et al., 2015</xref>; <xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al., 2016</xref>; <xref ref-type="bibr" rid="B37">Fernandez-Fernandez et al., 2018</xref>; <xref ref-type="bibr" rid="B70">Mohanta et al., 2019</xref>), no studies focused on female tennis players alone, two studies reported mixed gender (<xref ref-type="bibr" rid="B63">Lakshmikanth et al., 2018</xref>; <xref ref-type="bibr" rid="B52">Hotwani et al., 2021</xref>), and one study did not specify gender (<xref ref-type="bibr" rid="B40">Gelen et al., 2012</xref>); 2) Age: all studies recorded the participants&#x2019; ages, and an overview of age reports from twelve research revealed that the participants&#x2019; ages ranged from 12.5 to 25&#xa0;years; 3) Tennis experience: eight studies reported on the training experience of the tennis players (<xref ref-type="bibr" rid="B92">Salonikidis and Zafeiridis, 2008</xref>; <xref ref-type="bibr" rid="B40">Gelen et al., 2012</xref>; <xref ref-type="bibr" rid="B8">Behringer et al., 2013</xref>; <xref ref-type="bibr" rid="B34">Fernandez-Fernandez et al., 2015</xref>; <xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al., 2016</xref>; <xref ref-type="bibr" rid="B37">Fernandez-Fernandez et al.2018</xref>; <xref ref-type="bibr" rid="B70">Mohanta et al., 2019</xref>), ranging from 12 to 96&#xa0;months, four studies did not report on training experience (<xref ref-type="bibr" rid="B72">&#xd6;l&#xe7;&#xfc;c&#xfc; et al., 2013</xref>; <xref ref-type="bibr" rid="B86">Rathore, 2016</xref>; <xref ref-type="bibr" rid="B63">Lakshmikanth et al., 2018</xref>; <xref ref-type="bibr" rid="B52">Hotwani et al., 2021</xref>); 4) Intervention: regarding the training regimen in this review, four studies conducted PT for the upper and lower extremities (<xref ref-type="bibr" rid="B8">Behringer et al., 2013</xref>; <xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al., 2016</xref>; <xref ref-type="bibr" rid="B70">Mohanta et al., 2019</xref>; <xref ref-type="bibr" rid="B114">Ziagkas et al., 2019</xref>), and one study used only upper limb PT (<xref ref-type="bibr" rid="B40">Gelen et al., 2012</xref>), six studies used lower-extremity PT (<xref ref-type="bibr" rid="B92">Salonikidis and Zafeiridis, 2008</xref>; <xref ref-type="bibr" rid="B34">Fernandez-Fernandez et al., 2015</xref>; <xref ref-type="bibr" rid="B86">Rathore, 2016</xref>; <xref ref-type="bibr" rid="B37">Fernandez-Fernandez et al., 2018</xref>; <xref ref-type="bibr" rid="B63">Lakshmikanth et al., 2018</xref>; <xref ref-type="bibr" rid="B52">Hotwani et al., 2021</xref>), but three studies did not detailed description the training protocol (<xref ref-type="bibr" rid="B72">&#xd6;l&#xe7;&#xfc;c&#xfc;, 2013</xref>; <xref ref-type="bibr" rid="B86">Rathore, 2016</xref>; <xref ref-type="bibr" rid="B114">Ziagkas et al., 2019</xref>). In addition, three studies combined PT with other types of exercise training (i.e., sprint training, acceleration/deceleration/change of direction drills) (<xref ref-type="bibr" rid="B34">Fernandez-Fernandez et al., 2015</xref>; <xref ref-type="bibr" rid="B37">Fernandez-Fernandez et al., 2018</xref>; <xref ref-type="bibr" rid="B52">Hotwani et al., 2021</xref>). The frequency of training was one to three times per week, and two studies did not report the frequency (<xref ref-type="bibr" rid="B40">Gelen et al., 2012</xref>; <xref ref-type="bibr" rid="B52">Hotwani et al., 2021</xref>). The duration of the intervention was primarily between 20 and 60&#xa0;min, but one study did not report the period (<xref ref-type="bibr" rid="B92">Salonikidis and Zafeiridis, 2008</xref>). The interventions ranged from three to 9&#xa0;weeks, and most of the interventions were conducted for 8&#xa0;weeks (<italic>n</italic> &#x3d; 8), and only one study did not report the length (<xref ref-type="bibr" rid="B40">Gelen et al., 2012</xref>).</p>
</sec>
<sec id="s3-4">
<title>Results from meta-analysis</title>
<p>The meta-analysis was conducted on certain trials that measured maximal serve velocity (<italic>n</italic> &#x3d; 3), sprint speed (<italic>n</italic> &#x3d; 3), lower extremity power (<italic>n</italic> &#x3d; 3) and strength (<italic>n</italic> &#x3d; 3), agility (<italic>n</italic> &#x3d; 4). Other outcomes were insufficient and produced limited data for pooling, consequently, they were not considered for meta-analysis. The data used for meta-analyses and the forest plots are presented in <xref ref-type="sec" rid="s12">Supplementary Appendix S3, S5</xref>
<bold>,</bold> respectively.</p>
<p>The PT showed a significant increase (<italic>p</italic> &#x3c; 0.001) of maximal serve velocity with moderate effect (ES &#x3d; 0.75; 95% CI &#x3d; 0.38&#x2013;1.12; Egger&#x2019;s test <italic>p</italic> &#x3d; 1.00; <italic>n</italic> &#x3d; 115) and low heterogeneity (I<sup>2</sup> &#x3d; 0.0%) (<xref ref-type="sec" rid="s12">Supplementary Appendix S2</xref>; <xref ref-type="sec" rid="s12">Supplementary Figure S1</xref>).</p>
<p>The PT showed a significant increase (<italic>p</italic> &#x3d; 0.046) of sprint speed with moderate effect (ES &#x3d; 0.43; 95% CI &#x3d; 0.01&#x2013;0.85; Egger&#x2019;s test <italic>p</italic> &#x3d; 1.00; <italic>n</italic> &#x3d; 115) and low heterogeneity (I<sup>2</sup> &#x3d; 18.7%) (<xref ref-type="sec" rid="s12">Supplementary Appendix S2</xref>; <xref ref-type="sec" rid="s12">Supplementary Figure S2</xref>).</p>
<p>The PT showed a significant increase (<italic>p</italic> &#x3d; 0.022) of lower extremity power with small effect (ES &#x3d; 0.50; 95% CI &#x3d; 0.07&#x2013;0.93; Egger&#x2019;s test <italic>p</italic> &#x3d; 0.734; <italic>n</italic> &#x3d; 115) and lower heterogeneity (I<sup>2</sup> &#x3d; 19.9%) (<xref ref-type="sec" rid="s12">Supplementary Appendix S2</xref>; <xref ref-type="sec" rid="s12">Supplementary Figure S3</xref>).</p>
<p>The PT showed a non-significant change (<italic>p</italic> &#x3d; 0.115) in lower extremity muscle strength with small effect (ES &#x3d; 0.30; 95% CI &#x3d;-0.07&#x2013;0.68; Egger&#x2019;s test <italic>p</italic> &#x3d; 0.471; <italic>n</italic> &#x3d; 108) and lower heterogeneity (I<sup>2</sup> &#x3d; 0.0%) (<xref ref-type="sec" rid="s12">Supplementary Appendix S2</xref>; <xref ref-type="sec" rid="s12">Supplementary Figure S4</xref>).</p>
<p>The PT showed a significant increase (<italic>p</italic> &#x3c; 0.001) of agility with moderate effect (ES &#x3d; 0.88; 95% CI &#x3d; 0.53&#x2013;1.23; Egger&#x2019;s test <italic>p</italic> &#x3d; 0.060; <italic>n</italic> &#x3d; 105) and lower heterogeneity (I<sup>2</sup> &#x3d; 6.42%) (<xref ref-type="sec" rid="s12">Supplementary Appendix S2</xref>; <xref ref-type="sec" rid="s12">Supplementary Figure S5</xref>).</p>
</sec>
</sec>
<sec id="s4">
<title>Synthesis of results</title>
<sec id="s4-1">
<title>Effect of plyometric training on maximal serve velocity and accuracy</title>
<p>Five studies contained in this review evaluated maximal serve velocity. Among these studies, ball speed measurement by radar gun (<xref ref-type="bibr" rid="B40">Gelen et al., 2012</xref>; <xref ref-type="bibr" rid="B8">Behringer et al., 2013</xref>; <xref ref-type="bibr" rid="B72">&#xd6;l&#xe7;&#xfc;c&#xfc; et al., 2013</xref>; <xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al., 2016</xref>; <xref ref-type="bibr" rid="B37">Fernandez-Fernandez et al., 2018</xref>). Two RCTs measured this skill aspect. <xref ref-type="bibr" rid="B40">Gelen et al. (2012)</xref> compared EG3 and CG employing high-volume upper body PT <italic>vs</italic>. normal tennis practice. They discovered a significant (<italic>p</italic> &#x3d; 0.001) acute effect in maximal serve speed. <xref ref-type="bibr" rid="B8">Behringer et al. (2013)</xref> conducted an 8-week PT for the upper and lower bodies and increased the maximum serve velocity in a statistically significant (<italic>p</italic> &#x3c; 0.05) way. Three non-RCTs studied maximal serve velocity. <xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al. (2016)</xref> found a significant increase (<italic>p</italic> &#x3c; 0.05) in maximal serve velocity after an 8-week upper and lower body PT program. Meanwhile, in another study, the comparison of PT <italic>versus</italic> a traditional training regimen indicated positive effects on maximal serve velocity (<italic>p</italic> &#x3c; 0.01) (<xref ref-type="bibr" rid="B72">&#xd6;l&#xe7;&#xfc;c&#xfc; et al., 2013</xref>). In addition, the report by <xref ref-type="bibr" rid="B37">Fernandez-Fernandez et al. (2018)</xref> demonstrates that the EG1 significantly improved the maximal serve velocity. In contrast, the EG2 did not observe any changes.</p>
<p>One RCT (<xref ref-type="bibr" rid="B8">Behringer et al., 2013</xref>) and one non-RCT (<xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al., 2016</xref>) measured serve accuracy. The accuracy of the service is scored by calculating the position of the ball landing in the specified target area. The RCT reported neither positive nor adverse impacts have been observed on service accuracy scores after an 8-week PT program (<xref ref-type="bibr" rid="B8">Behringer et al., 2013</xref>). Interestingly, <xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al. (2016)</xref> reported a significant change (<italic>p</italic> &#x3c; 0.01) in serve accuracy after a similar 8-week PT program.</p>
</sec>
<sec id="s4-2">
<title>Effect of plyometric training on sprint speed</title>
<p>Five of the studies (one RCT and four non-RCTs) assessed sprint speed. The sprint tests used in these investigations comprised the linear sprint test of 4&#xa0;m (<xref ref-type="bibr" rid="B92">Salonikidis and Zafeiridis, 2008</xref>), 5&#xa0;m (<xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al., 2016</xref>), 10&#xa0;m (<xref ref-type="bibr" rid="B34">Fernandez-Fernandez et al., 2015</xref>; <xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al., 2016</xref>; <xref ref-type="bibr" rid="B37">Fernandez-Fernandez et al., 2018</xref>), 12&#xa0;m (<xref ref-type="bibr" rid="B92">Salonikidis and Zafeiridis, 2008</xref>), 20&#xa0;m (<xref ref-type="bibr" rid="B34">Fernandez-Fernandez et al., 2015</xref>; <xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al., 2016</xref>; <xref ref-type="bibr" rid="B37">Fernandez-Fernandez et al., 2018</xref>), 30&#xa0;m (<xref ref-type="bibr" rid="B34">Fernandez-Fernandez et al., 2015</xref>), 50&#xa0;m (<xref ref-type="bibr" rid="B70">Mohanta et al., 2019</xref>); lateral sprint test of 4&#xa0;m (<xref ref-type="bibr" rid="B92">Salonikidis and Zafeiridis, 2008</xref>); and change of direction sprint test of 12&#xa0;m with turn (<xref ref-type="bibr" rid="B92">Salonikidis and Zafeiridis, 2008</xref>), repeated sprint ability (<xref ref-type="bibr" rid="B34">Fernandez-Fernandez et al., 2015</xref>).</p>
<p>The RCT reveals that it significantly improves (<italic>p</italic> &#x3c; 0.05) 12&#xa0;m sprint without turn performance in the combined group (PT &#x2b; regular tennis drills) and PT alone group. Meanwhile, the performance in the 12&#xa0;m sprint with turn was improved only in the EG2 and failed to reach significant differences in the EG1 (<xref ref-type="bibr" rid="B92">Salonikidis and Zafeiridis. 2008</xref>). Three 8-week non-RCTs revealed improvements (<italic>p</italic> &#x3c; 0.05) in the linear sprint test (<xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al., 2016</xref>; <xref ref-type="bibr" rid="B70">Mohanta et al., 2019</xref>) and repeated sprint ability test (<xref ref-type="bibr" rid="B34">Fernandez-Fernandez et al., 2015</xref>). Of note, <xref ref-type="bibr" rid="B34">Fernandez-Fernandez et al. (2015)</xref> reported that the 10&#xa0;m test was significantly improved (<italic>p</italic> &#x3c; 0.05), in contrast to the 20&#xa0;m test and 30&#xa0;m test. <xref ref-type="bibr" rid="B37">Fernandez-Fernandez et al. (2018)</xref> reported that the EG1 had a positive effect on pre-to post-test measures of the sprint, while the EG2 training method had negative effects on 5&#xa0;m, or trivial effects on 10&#xa0;m and 20&#xa0;m.</p>
</sec>
<sec id="s4-3">
<title>Effect of plyometric training on upper and lower extremity power</title>
<p>Two non-RCTs measured upper extremity power <italic>via</italic> medicine ball throw tests (<xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al., 2016</xref>; <xref ref-type="bibr" rid="B37">Fernandez-Fernandez et al., 2018</xref>). <xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al. (2016)</xref> compared EG and CG applying an 8-week PT program <italic>vs</italic>. normal tennis practice. They discovered that MBT performance had improved significantly (<italic>p</italic> &#x3c; 0.05). <xref ref-type="bibr" rid="B37">Fernandez-Fernandez et al., 2018</xref> observed a considerable increase in MBT following the EG1 training method, but no improvement in EG2.</p>
<p>One RCT (<xref ref-type="bibr" rid="B92">Salonikidis and Zafeiridis, 2008</xref>) and four non-RCTs (<xref ref-type="bibr" rid="B34">Fernandez-Fernandez et al., 2015</xref>; <xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al., 2016</xref>; <xref ref-type="bibr" rid="B37">Fernandez-Fernandez et al., 2018</xref>; <xref ref-type="bibr" rid="B70">Mohanta et al., 2019</xref>) measured lower extremity power. This outcome test consisted of CMJ test (<xref ref-type="bibr" rid="B34">Fernandez-Fernandez et al., 2015</xref>; <xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al., 2016</xref>; <xref ref-type="bibr" rid="B37">Fernandez-Fernandez et al., 2018</xref>), drop jump test (<xref ref-type="bibr" rid="B92">Salonikidis and Zafeiridis, 2008</xref>), SLJ test (<xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al., 2016</xref>), VJ test (<xref ref-type="bibr" rid="B70">Mohanta et al., 2019</xref>). <xref ref-type="bibr" rid="B92">Salonikidis and Zafeiridis (2008)</xref> found that EG2 activities had a significant gain (<italic>p</italic> &#x3c; 0.05) in DJ height when compared to EG1. <xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al. (2016)</xref> demonstrate that EG <italic>vs</italic>. CG significantly enhanced (<italic>p</italic> &#x3c; 0.01) CMJ and SLJ performance. <xref ref-type="bibr" rid="B34">Fernandez-Fernandez et al. (2015)</xref> compared EG and CG using an 8-week combination of explosive strength and repeated sprint training. The results indicate that CMJ achieved a positive effect. <xref ref-type="bibr" rid="B37">Fernandez-Fernandez et al. (2018)</xref> reported data on EG1 <italic>vs</italic>. EG2, and it is noted that the EG1 training approach markedly improved (<italic>p</italic> &#x3c; 0.05) the CMJ performance, in contrast to the EG2. Furthermore, <xref ref-type="bibr" rid="B70">Mohanta et al. (2019)</xref> showed that 8&#xa0;weeks of PT could significantly improve VJ height (<italic>p</italic> &#x3d; 0.027).</p>
</sec>
<sec id="s4-4">
<title>Effect of plyometric training on upper and lower extremity strength</title>
<p>One RCT (<xref ref-type="bibr" rid="B8">Behringer et al., 2013</xref>) and two non-RCTs (<xref ref-type="bibr" rid="B72">&#xd6;l&#xe7;&#xfc;c&#xfc; et al., 2013</xref>; <xref ref-type="bibr" rid="B70">Mohanta et al., 2019</xref>) measured upper extremity strength. The tests applied at this point involved the 1RM and 10RM chest press test (<xref ref-type="bibr" rid="B8">Behringer et al., 2013</xref>; <xref ref-type="bibr" rid="B70">Mohanta et al., 2019</xref>), and the torque of the shoulder joint test (<xref ref-type="bibr" rid="B72">&#xd6;l&#xe7;&#xfc;c&#xfc; et al., 2013</xref>). The RCT (8-week PT program) conducted by <xref ref-type="bibr" rid="B8">Behringer et al. (2013)</xref> observed a greater increase (<italic>p</italic> &#x3c; 0.05) in upper extremity strength (10RM chest press test) when compared to the control group. Two similar 8-week PT programs (non-RCT design) also found a statistically significant difference (<italic>p</italic> &#x3c; 0.05) in upper limb strength (1RM chest press and torque of the shoulder joint test) measurement between the experimental group&#x2019;s pre-and post-test (<xref ref-type="bibr" rid="B72">&#xd6;l&#xe7;&#xfc;c&#xfc; et al., 2013</xref>; <xref ref-type="bibr" rid="B70">Mohanta et al., 2019</xref>).</p>
<p>The lower extremity strength test consists of a 10RM leg press test (<xref ref-type="bibr" rid="B8">Behringer et al., 2013</xref>), a maximum isometric force test (leg) (<xref ref-type="bibr" rid="B92">Salonikidis and Zafeiridis, 2008</xref>), and a torque of the knee joint test (<xref ref-type="bibr" rid="B72">&#xd6;l&#xe7;&#xfc;c&#xfc; et al., 2013</xref>). Two RCTs indicated that tennis players who trained with 8&#xa0;weeks of PT had considerably larger increases in lower extremity strength (<italic>p</italic> &#x3c; 0.05) than the control group (<xref ref-type="bibr" rid="B92">Salonikidis and Zafeiridis, 2008</xref>; <xref ref-type="bibr" rid="B8">Behringer et al., 2013</xref>). One non-RCT also achieved a positive effect in the strength test (<xref ref-type="bibr" rid="B72">&#xd6;l&#xe7;&#xfc;c&#xfc; et al., 2013</xref>) after 8-week PT.</p>
</sec>
<sec id="s4-5">
<title>Effect of plyometric training on agility</title>
<p>In the twelve studies that were reviewed, agility was reported in seven. The factors valued and assessment instruments used included the 5-0-5 test (<xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al., 2016</xref>; <xref ref-type="bibr" rid="B37">Fernandez-Fernandez et al., 2018</xref>), T-test (<xref ref-type="bibr" rid="B70">Mohanta et al., 2019</xref>; <xref ref-type="bibr" rid="B52">Hotwani et al., 2021</xref>), Illinois test (<xref ref-type="bibr" rid="B86">Rathore, 2016</xref>; <xref ref-type="bibr" rid="B63">Lakshmikanth et al., 2018</xref>; <xref ref-type="bibr" rid="B52">Hotwani et al., 2021</xref>), tennis-specific agility test (<xref ref-type="bibr" rid="B63">Lakshmikanth et al., 2018</xref>), hexagon test and spider test (<xref ref-type="bibr" rid="B114">Ziagkas et al., 2019</xref>). Two RCTs (<xref ref-type="bibr" rid="B86">Rathore, 2016</xref>; <xref ref-type="bibr" rid="B114">Ziagkas et al., 2019</xref>) show that EG <italic>vs</italic>. CG substantially increased agility performance (hexagon test, spider test, Illinois test; <italic>p</italic> &#x3c; 0.0001). Among non-RCTs, a statistically significant difference between the pre-and post-test in several agility tests (i.e., 5-0-5 test, T-test, Illinois test, tennis-specific agility test) was revealed by the EG of studies (<xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al., 2016</xref>; <xref ref-type="bibr" rid="B63">Lakshmikanth et al., 2018</xref>; <xref ref-type="bibr" rid="B70">Mohanta et al., 2019</xref>). Moreover, <xref ref-type="bibr" rid="B52">Hotwani et al., 2021</xref> revealed that 3&#xa0;weeks of combining plyometric with sprint training sessions resulted in an extremely significant (<italic>p</italic> &#x3c; 0.0001) gain in agility (Illinois test and T-test). However, <xref ref-type="bibr" rid="B37">Fernandez-Fernandez et al. (2018)</xref> discovered positive effects from pre-test to post-test measures on agility (5-0-5 test) in the EG1. On the contrary, the agility test for the EG2 showed negative impacts.</p>
</sec>
<sec id="s4-6">
<title>Effect of plyometric training on reaction time/aerobic endurance</title>
<p>One RCT (<xref ref-type="bibr" rid="B92">Salonikidis and Zafeiridis, 2008</xref>) and one non-RCT (<xref ref-type="bibr" rid="B34">Fernandez-Fernandez et al., 2015</xref>) evaluated reaction time and aerobic endurance performance, respectively.</p>
<p>The reaction time was measured based on the load cells. <xref ref-type="bibr" rid="B92">Salonikidis and Zafeiridis 2008</xref> reported outcomes on EG1 <italic>vs</italic>. EG2; both PT regimens induce favorable changes to reaction time performance (<italic>p</italic> &#x3c; 0.05). Another study using the 30&#x2013;15 interval fitness test evaluated aerobic endurance performance, and the study reported no significant improvement in this aspect of factor (<xref ref-type="bibr" rid="B34">Fernandez-Fernandez et al., 2015</xref>).</p>
</sec>
<sec id="s4-7">
<title>Adverse effects</title>
<p>It is worthy to note that one out of the twelve studies reported that eight players in the control group and one in the PT group were dropped from the final collection of data because of acute injuries (i.e., ankle sprain) (<xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al., 2016</xref>). Apart from that, no other studies in this review reported fatigue, soreness, injury, pain, damage, or adverse effects associated with PT intervention.</p>
</sec>
</sec>
<sec sec-type="discussion" id="s5">
<title>Discussion</title>
<p>This is the first meta-analysis to investigate the impact of PT on healthy tennis players. This study involved twelve trials, and five studies involved skill, but only serve performance. Eleven articles were related to physical performance. Besides, no data were found on body composition, balance, flexibility, and other aspects of skills (<xref ref-type="table" rid="T2">Table 2</xref>). The results demonstrated that PT interventions induced small-to-moderate improvements (ES &#x3d; 0.43&#x2013;0.88) in tennis players&#x2019; maximal serve velocity and physical performance parameters (i.e., sprint speed, lower body power, agility), and a non-significant, small improvement (ES &#x3d; 0.30) in lower extremity strength. In most cases, the results indicated above had a low level of heterogeneity (I<sup>2</sup> &#x3d; 0.0%&#x2013;19.9%). In this review, one study reported acute injuries during the intervention which the authors had recorded. It is still necessary that coaches and players remain cautious and vigilant about injuries sustained during PT drills, and that the training must be performed under supervision. Furthermore, only two of the twelve studies in this work were rated with a low risk of bias. Additionally, a very low to moderate level of evidence was reported for the measured variables. Therefore, the outcomes should be interpreted with caution.</p>
<sec id="s5-1">
<title>Effect of plyometric training on maximal serve velocity and accuracy</title>
<p>The tennis serve is undoubtedly one of the most challenging tennis shots to master, but it can significantly contribute to a point win or advantage (<xref ref-type="bibr" rid="B43">Guillot et al., 2013</xref>). Based on our meta-analysis, PT interventions were found to have a moderate effect (ES &#x3d; 0.75) on maximal serve velocity in tennis players. The serve is a complicated stroke that involves a sequence of forces (legs, trunk, and arm/racquet) moving from proximal to distal (<xref ref-type="bibr" rid="B28">Elliott, 2006</xref>). During a serve, it is crucial to transfer power from the legs, trunk, and arm to the ball as quickly as possible to maximize the velocity (<xref ref-type="bibr" rid="B109">van den Tillaar. 2004</xref>). According to <xref ref-type="bibr" rid="B38">Ferrauti and Bastiaens, (2007)</xref>, service velocity results from an efficient force transfer along a convoluted kinetic chain dependent on intermuscular coordination and muscle strength. Simultaneously, PT helps to increase muscular strength and intermuscular coordination, so that force transfer during service can be improved (<xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al., 2016</xref>). Previously, resistance training has been recommended to increase ball velocity in tennis (<xref ref-type="bibr" rid="B116">Kraemer et al., 2000</xref>; <xref ref-type="bibr" rid="B61">Kraemer et al., 2003</xref>); yet, a study in the current review specifically compared the effects of PT and resistance training on this factor and found that serve speed improvement was significantly higher in the PT group than in the resistance training group (<xref ref-type="bibr" rid="B8">Behringer et al., 2013</xref>). In addition, <xref ref-type="bibr" rid="B33">Fernandez-Fernandez et al., 2013</xref> suggested that upper-extremity plyometric exercises can be frequently used by athletes in the pursuit of more powerful functional performance. In the present review, of the five studies measuring serve speed, four designed upper-body plyometrics in the training protocols (<xref ref-type="sec" rid="s12">Supplementary Appendix S4</xref>), therefore, the increase in maximal serve velocity was expected.</p>
<p>In tennis, the main determinants of shot quality include not only ball velocity but also ball placement accuracy (<xref ref-type="bibr" rid="B60">Kovacs and Ellenbecker, 2011</xref>). <xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al. (2016)</xref> speculated that the improved kinetic chain as a result of the power gains from the PT would contribute to the stability of tennis players from a technical perspective, leading to a higher accuracy test score. However, another study found that similar plyometric exercises did not affect serve accuracy (<xref ref-type="bibr" rid="B8">Behringer et al., 2013</xref>). In the literature, service accuracy was also unaffected by other forms of strength training (<xref ref-type="bibr" rid="B38">Ferrauti and Bastiaens, 2007</xref>; <xref ref-type="bibr" rid="B33">Fernandez-Fernandez et al., 2013</xref>). <xref ref-type="bibr" rid="B104">Terraza-Rebollo and Baiget. (2020)</xref> explained in their study that it was likely because subjects were instructed to hit the ball at maximum speed, so it was difficult to take into account the accuracy. However, there is some controversy because it has also been observed a positive correlation between velocity and accuracy in tennis (<xref ref-type="bibr" rid="B15">Cauraugh et al., 1990</xref>).</p>
<p>Therefore, PT is an effective method that may have greater chances of improvement in tennis players&#x2019; maximal serve velocity. Concerning serve accuracy, it was impossible to reach a firm conclusion, because only two studies examined the effect of PT on this point. Furthermore, more research is required to explore the relationship between training-induced increases in serve velocity and accuracy.</p>
</sec>
<sec id="s5-2">
<title>Effect of plyometric training on sprint speed</title>
<p>Better sprint performance will enable tennis players to get to the ball faster and will give them more time to prepare for the shot (<xref ref-type="bibr" rid="B62">Kramer et al., 2021</xref>). The meta-analysis, summarizing the effects of PT on the sprint speed performance of tennis players, showed a small but significant effect across studies (ES &#x3d; 0.43). Increases in sprint performance following PT may be due to increased neuromuscular activation of the exercised muscles (<xref ref-type="bibr" rid="B44">Hakkinen and Komi, 1985</xref>). Moreover, these adaptations induced by lower body plyometric exercises (e.g., sprinting, hopping, jumping), such as improving muscle-tendon stiffness and increasing neural drive to agonist muscles (<xref ref-type="bibr" rid="B68">Markovic and Mikulic, 2010</xref>) may improve SSC efficacy. As a result of gains in SSC efficacy in lower limb musculature, stronger force generation likely occurs in the concentric action phase after a rapid eccentric muscle movement (<xref ref-type="bibr" rid="B68">Markovic and Mikulic., 2010</xref>; <xref ref-type="bibr" rid="B77">Radnor et al., 2018</xref>), which is a key requirement for enhancing tennis players&#x2019; sprint performance. However, one study found that PT had no effect on a 12&#xa0;m sprint with a turn test (<xref ref-type="bibr" rid="B92">Salonikidis and Zafeiridis, 2008</xref>). This observation may have resulted from the activity being conducted only on one leg (<xref ref-type="bibr" rid="B92">Salonikidis and Zafeiridis, 2008</xref>). Furthermore, <xref ref-type="bibr" rid="B34">Fernandez-Fernandez et al. (2015)</xref> concluded that a PT program did not result in significant gains in the 20&#xa0;m and 30&#xa0;m sprint measurements. This discovery might be connected to the athletes&#x2019; stride frequency and the coordination of the lower limb muscles (<xref ref-type="bibr" rid="B113">Young et al., 2001</xref>). In fact, only a few studies in the literature did not report the positive effects of PT on sprint performance (<xref ref-type="bibr" rid="B73">Oxfeldt et al., 2019</xref>). For example, <xref ref-type="bibr" rid="B46">Hammami et al. (2019)</xref> investigated the impact of a night-week PT program on young female handball players. There were no changes reported for the 5&#xa0;m and 10&#xa0;m sprint times, which could be explained by the fact that initial acceleration (over 5 and 10&#xa0;m) has proven to be more difficult to improve than maximal velocity, most likely due to the smaller margin for improvement and the different forces involved. Interestingly, <xref ref-type="bibr" rid="B47">Hammami et al. (2020)</xref> found inconsistent results on young female handball players, that there were significant increases in speed over distances of 5&#x2013;30&#xa0;m after 10&#xa0;weeks of similar plyometric exercises. Differences between PT programs (e.g., frequency, duration) may help to explain the different magnitudes of physical fitness changes among studies (<xref ref-type="bibr" rid="B82">Ramirez-Campillo et al., 2020b</xref>). However, the paucity of available data limits our attempts to explore the effects of these factors on training effects. Therefore, although we have found evidence to support the use of PT as an effective training modality to improve sprint performance, more high-quality research on sprint speed performance is still needed.</p>
</sec>
<sec id="s5-3">
<title>Effect of plyometric training on upper and lower extremity muscle power</title>
<p>Power development is paramount, irrespective of the sport and proportion of each energy system engaged, since certain critical actions are executed as quickly and forcefully as possible (<xref ref-type="bibr" rid="B29">Elliott et al., 2007</xref>). On the one hand, upper extremity power was studied in two studies included in this review, and this variable was examined using medicine ball throws. These outcomes showed positive effects (<xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al., 2016</xref>; <xref ref-type="bibr" rid="B37">Fernandez-Fernandez et al., 2018</xref>). Ulbricht and others discovered that the upper extremity power test (MBT) was an important predictor of tennis performance (<xref ref-type="bibr" rid="B108">Ulbricht et al., 2016</xref>). These two studies in our review incorporating PT of the upper limb involving multiple medicine ball exercises could be an important factor in improving upper extremity power. Furthermore, PT works by utilizing the natural elastic components of muscles and tendons, and stretch reflexes, to increase the power of subsequent movements (<xref ref-type="bibr" rid="B106">Trajkovic et al., 2016</xref>).</p>
<p>On the other hand, a meta-analysis of lower extremity power (CMJ) was undertaken, and the PT indicated a significant increase with a small effect (ES &#x3d; 0.50). Such effects were noted for participants with a wide range of sports backgrounds. A meta-analysis on individual sports athletes was done by <xref ref-type="bibr" rid="B99">Sole et al. (2021)</xref>, and the results showed similar improvements in the CMJ performance of ES &#x3d; 0.49. In short, factors including improved motor unit recruitment, increased intermuscular coordination, increased neural drive to the muscles of the agonist, and improved SSC utilization are responsible for jump performance improvements (<xref ref-type="bibr" rid="B68">Markovic and Mikulic, 2010</xref>; <xref ref-type="bibr" rid="B102">Taube et al., 2012</xref>). Of note, a study conducted by <xref ref-type="bibr" rid="B37">Fernandez-Fernandez et al. (2018)</xref> showed that performing NMT before regular tennis practice produced a substantially significant positive effect on CMJ. Conversely, when NMT was conducted after regular tennis drills, there was no improvement in CMJ assessed. The previous tennis regular practice that resulted in acute fatigue may have contributed to the lack of a marked increase in CMJ (<xref ref-type="bibr" rid="B39">Garcia-Pallares and Izquierdo, 2011</xref>). From this, the optimal design and implementation of training strategies are significant for tennis players. This should be taken into account in future research.</p>
<p>Overall, our findings imply that PT positively affects tennis players&#x2019; lower extremity power, and future research should consider further research on upper extremity power.</p>
</sec>
<sec id="s5-4">
<title>Effect of plyometric training on upper and lower extremity muscle strength</title>
<p>For successful athletic performance, strength is essential (<xref ref-type="bibr" rid="B97">Slimani et al., 2016</xref>). Moreover, it has been reported that a tennis player&#x2019;s upper and lower body strength can be very helpful in preventing injuries (<xref ref-type="bibr" rid="B27">Ellenbecker and Roetert, 2004</xref>). Of the four studies included in this review assessing strength, three reported upper body strength. These studies showed significant improvement in upper extremity strength tests (<xref ref-type="bibr" rid="B72">&#xd6;l&#xe7;&#xfc;c&#xfc; et al., 2013</xref>, <xref ref-type="bibr" rid="B8">Behringer et al., 2013</xref>; <xref ref-type="bibr" rid="B70">Mohanta et al., 2019</xref>).</p>
<p>Meanwhile, it has been found in the present meta-analysis that PT led to small gains in lower extremity muscle strength (ES &#x3d; 0.30), but this was not statistically significant. In contrast, <xref ref-type="bibr" rid="B22">de Villarreal et al. (2010)</xref> observed that PT has a significant, moderate effect on lower limb muscle strength (ES &#x3d; 0.97). Theoretically, PT increases cross-bridge mechanics, activates motor units, enhances neural efficiency, and provides passive tension to the muscle-tendon complex, contributing to strength performance (<xref ref-type="bibr" rid="B67">Malisoux, 2005</xref>; <xref ref-type="bibr" rid="B79">Ram&#xed;rez-Campillo et al., 2015</xref>). Moreover, improvements in muscle strength after PT may also be associated with muscle hypertrophy (<xref ref-type="bibr" rid="B42">Grgic et al., 2020</xref>).</p>
<p>Additionally, the narrative synthesis in our review showed a positive direction of evidence for muscle strength, but the certainty of the evidence was very low to low. As a result, these findings are unclear, though this could be due to the relatively few studies conducted in this field. Likewise, although our meta-analysis results do not show a significant effect from PT on lower extremity muscle strength, numerous previous studies have concluded that PT is an efficient training technique to enhance strength performance in other ball players, such as basketball players (<xref ref-type="bibr" rid="B84">Ramirez-Campillo et al., 2021a</xref>), handball players (<xref ref-type="bibr" rid="B47">Hammami et al., 2020</xref>). Therefore, to confirm the effects of PT on tennis players, further high-quality studies simultaneously evaluating the influence of upper and lower body muscle strength are needed to draw more solid conclusions.</p>
</sec>
<sec id="s5-5">
<title>Effect of plyometric training on agility</title>
<p>The basic requirement for a tennis player is the ability to quickly switch between multidirectional movements (e.g., vertical, lateral, forward, backward) (<xref ref-type="bibr" rid="B86">Rathore, 2016</xref>). In the present review, one of the most studied physical attributes is agility (seven papers). The findings in the current meta-analysis show that PT has a significant, moderate effect (ES &#x3d; 0.88) on agility. Similar improvement in agility performance was observed by <xref ref-type="bibr" rid="B4">Asadi et al. (2016)</xref> and <xref ref-type="bibr" rid="B105">Thapa et al. (2021)</xref>, supporting the findings of our study. The present research evaluated agility performance using a variety of tests (5-0&#x2013;5 test, T-test, Illinois test, Heroxge test, tennis-specific agility test). Of note, <xref ref-type="bibr" rid="B4">Asadi et al. (2016)</xref> suggested that PT could improve change-of-direction ability (ES &#x3d; 0.26&#x2013;2.8, small-to-large), depending on the types of plyometric exercises and change-of-direction tests. The current results are consonant with these findings, with extensively positive results in all of the agility tests after PT.</p>
<p>Additionally, <xref ref-type="bibr" rid="B37">Fernandez-Fernandez et al. (2018)</xref> found positive effects from pre-test to post-test analyzes on agility in the EG1. In contrast, the EG2 observed negative effects on agility. As aforementioned, the fatigue of regular tennis training may have contributed to the decline in agility. In the literature, it has been well identified that PT is a time-efficient, effective and simple method for improving agility among athletes (<xref ref-type="bibr" rid="B5">Asadi, 2013</xref>; <xref ref-type="bibr" rid="B3">Arazi et al., 2014</xref>; <xref ref-type="bibr" rid="B78">Ram&#xed;rez-Campillo et al., 2014</xref>). Indeed, PT helps reduce ground contact by boosting muscular force output and movement efficiency, which is related to agility improvement (<xref ref-type="bibr" rid="B68">Markovic and Mikulic, 2010</xref>; <xref ref-type="bibr" rid="B4">Asadi et al., 2016</xref>). Moreover, this type of training approach could have increased eccentric strength in the legs, allowing athletes better to switch between deceleration and acceleration movements (<xref ref-type="bibr" rid="B13">Brughelli et al., 2008</xref>). Meanwhile, plyometric exercises which included powerful multidirectional movements helped improve the ability to change directions rapidly (<xref ref-type="bibr" rid="B98">S&#xf6;hnlein et al., 2014</xref>). Therefore, the findings suggest that PT might assist tennis players to enhance their agility performance.</p>
</sec>
<sec id="s5-6">
<title>Effect of plyometric training on reaction time/aerobic endurance</title>
<p>The tennis player&#x2019;s ability to react quickly in response to their competitor&#x2019;s movements is crucial during a game (<xref ref-type="bibr" rid="B87">Reid et al., 2013</xref>). Reaction time performance has only been investigated in one study, and this study reported significant improvement in the reaction time test (<xref ref-type="bibr" rid="B92">Salonikidis and Zafeiridis, 2008</xref>). Similar results were found in a study done by <xref ref-type="bibr" rid="B107">Turgut et al. (2019)</xref> which showed a significant improvement in reaction time as a result of PT. However, discussion on the mechanisms related to improved reaction time in athletes after PT remains unclear, with extensive empirical research required to elucidate such mechanisms.</p>
<p>Likewise, aerobic endurance is a major component of physical fitness (<xref ref-type="bibr" rid="B110">Wezenberg et al., 2013</xref>), and can be considered one of the benefits of PT. However, only one paper examined aerobic endurance in the current review, and this study found no significant improvement in the aerobic endurance test (30&#x2013;15 interval fitness test) (<xref ref-type="bibr" rid="B35">Fernandez-Fernandez et al., 2016</xref>). This may be due to repetitive short-duration exercises which induced changes in glycolytic enzymes, muscle buffering, and ion regulation and led to improved anaerobic capacity (<xref ref-type="bibr" rid="B21">Dawson, 2012</xref>) rather than aerobic performance.</p>
<p>Based on two articles, we cannot get more information from these two variables, thus, in the future, more studies evaluating the effects of PT on reaction time and aerobic endurance are required to reach more reliable conclusions.</p>
</sec>
<sec id="s5-7">
<title>Limitations</title>
<p>This systematic review can be regarded as having some limitations, which should be taken into account. The first limitation is that only 12 publications were analyzed, and there were insufficient RCTs (<italic>n</italic> &#x3d; 5) included in this study. Secondly, there is no study specifically analyzing female tennis players in this review, which limits our comprehension of the overall effectiveness of PT in tennis players. This presents a crucial gap in the study, and should be addressed in further studies. Thirdly, three studies were excluded from the current meta-analysis because they lacked control group data or did not offer enough. In addition, several outcomes (such as serve accuracy, upper extremity power and strength) were excluded from our meta-analysis, since there was an insufficient number of studies involved. Fourthly, several studies did not give a comprehensive description of the training program; for example, three of the included publications did not include details regarding the training protocol. Fifthly, the majority of the studies included in this review had small sample sizes (8&#x2013;30 people per EG), and only one research disclosed the sample size calculation technique (<xref ref-type="bibr" rid="B70">Mohanta et al., 2019</xref>), meaning statistically significant results may not reflect the actual effect. As demonstrated by GRADE, the certainty of the evidence ranged from very low to moderate for most of the study outcomes, lowering confidence in the reported estimates.</p>
</sec>
</sec>
<sec sec-type="conclusion" id="s6">
<title>Conclusion</title>
<p>To summarize, this systematic review and meta-analysis demonstrated that PT implicates muscle stimulus that induces neuro-mechanical adaptations, which could increase force production, thereby improving tennis performance, including maximal serve velocity and physical performance components (i.e., sprint speed, lower extremity muscle power, agility). There was no definitive evidence that PT changed serve accuracy, upper extremity power and strength, reaction time, and aerobic endurance. According to GRADE, the certainty of evidence is very low to moderate in studies. Future high-quality evidence is needed to demonstrate the effects of PT on skill and physical performance in tennis players.</p>
</sec>
<sec id="s7">
<title>Practical application</title>
<p>The findings of this review have practical implications for athletic trainers, coaches, and athletes. PT might be advised as a training method to boost maximal serve velocity or different parameters of physical performance in healthy tennis players. These factors are essential for enhancing the competitive level of tennis players (<xref ref-type="bibr" rid="B64">Lambrich and Muehlbauer, 2022</xref>). Moreover, one of the primary advantages of this type of training is that it requires only inexpensive equipment (e.g., jump box, medicine ball), so it can be readily incorporated into the daily training regimen (<xref ref-type="bibr" rid="B33">Fernandez-Fernandez et al., 2013</xref>). Regarding the characteristics of effective PT interventions, it appears that a training frequency of two to three sessions per week for six to nine weeks is a sufficient stimulus to obtain improvement. However, due to the limited number of high-quality research conducted on the topic, specific recommendations cannot be made. More researchers are encouraged to explore the effects of PT on the athletic performance of tennis players.</p>
</sec>
</body>
<back>
<sec sec-type="data-availability" id="s8">
<title>Data availability statement</title>
<p>The original contributions presented in the study are included in the article/<xref ref-type="sec" rid="s12">Supplementary Material</xref>, further inquiries can be directed to the corresponding authors.</p>
</sec>
<sec id="s9">
<title>Author contributions</title>
<p>All authors contributed to the study&#x2019;s conception and design. Data synthesis and analysis were performed by ND and DH, in which study exclusion and data extraction was verified by three authors (ND, DH, and KS). The first draft of the manuscript was written by ND and all authors reviewed and contributed to the drafts and the final version of the submitted manuscript.</p>
</sec>
<sec sec-type="COI-statement" id="s10">
<title>Conflict of interest</title>
<p>The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.</p>
</sec>
<sec sec-type="disclaimer" id="s11">
<title>Publisher&#x2019;s note</title>
<p>All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.</p>
</sec>
<sec id="s12">
<title>Supplementary material</title>
<p>The Supplementary Material for this article can be found online at: <ext-link ext-link-type="uri" xlink:href="https://www.frontiersin.org/articles/10.3389/fphys.2022.1024418/full#supplementary-material">https://www.frontiersin.org/articles/10.3389/fphys.2022.1024418/full&#x23;supplementary-material</ext-link>
</p>
<supplementary-material xlink:href="DataSheet1.ZIP" id="SM1" mimetype="application/ZIP" xmlns:xlink="http://www.w3.org/1999/xlink"/>
</sec>
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