AUTHOR=Ghori Ifra , Tubassam Misbah , Ahmad Tanveer , Zuberi Amina , Imran Muhammad 

TITLE=Gut microbiome modulation mediated by probiotics: Positive impact on growth and health status of Labeo rohita

JOURNAL=Frontiers in Physiology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2022.949559

DOI=10.3389/fphys.2022.949559

ISSN=1664-042X

ABSTRACT=The current study was targeted to determine the effect of probiotics on the growth, physiology, and gut microbiology of Labeo rohita fingerlings. One hundred and twenty fishes were divided into four dietary groups each in triplicate for a feeding trial of 90 days. These treatments included: T0 (Control, basal diet) used as reference, and three probiotic supplemented diets represented as Tbc (Bacillus cereus), Tgc (Geotrichum candidum) and Tmc (B. cereus and G. candidum).The probiotics were supplemented at a level of 1 × 109 CFU/g feed. Fishes nurtured on probiotic added diet showed significantly high (P<0.05) physiological improvement (P<0.05) in terms of growth, feed utilization capacity hematological profile, enzymes titers as compared to control. The Tmc exhibiting maximum fish growth when challenged by to Staphylococcus aureus showed fish survival when compared to control in which fish mortalily was examined. Fish gut microbial composition was modulated by probiotic treatments, especially in Tgc and Tmc as compared to control. The absence of opportunistic pathogens such as Staphylococcus saprophyticus , Sporobolomyces lactosus and the detection of  low percentage of Trichosporon and Cryptococcus in treated groups indicates the gut modulation driven by applied probiotics. G. candidum QAUGC01 was retrieved in yeast metagenomics data which might be due to the production of polyamines by them that facilitated in adherence and consequent persistence. In conclusion it can be suggested that the probiotic supplemented diet could enhance fish growth profile and feed efficiency through community modulation and digestive enzymes thus could be a milestone in local aquaculture.