AUTHOR=Meng Xiao-Yan , Wang Dian-Dian , Xie Tao-Rong , Yang Run-Zhou , Liu Chun-Feng , Liu Dan-Hua , Li Shu-Ang , Luan Yi , Kang Jian-Sheng 

TITLE=A sensitive mitochondrial thermometry 2.0 and the availability of thermogenic capacity of brown adipocyte

JOURNAL=Frontiers in Physiology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2022.977431

DOI=10.3389/fphys.2022.977431

ISSN=1664-042X

ABSTRACT=The temperature of a living cell is a crucial parameter for cellular events, such as cell division, gene expressions, enzyme activities and metabolism. We previously developed a quantifiable mitochondrial thermometry 1.0 based on rhodamine B methyl ester (RhB-ME) and rhodamine 800 (Rh800), and deduced the theory for mitochondrial thermogenesis. Given that RhB-ME needs de novo synthesis so that it was not readily available, thus, we further evolved a convenient and sensitive mitochondrial thermometry 2.0 based on tetra-methyl rhodamine methyl ester (TMRM) and Rh800 for the thermogenic study of brown adipocyte. TMRM was demonstrated to be more sensitive (~1.4 times) to temperature than RhB-ME, and thus the TMRM-based mito-thermometry 2.0, which could be easily used for the qualitatively dynamic profiles for mitochondrial temperature and membrane potential in living cells simultaneously. Therefore, TMRM-based mito-thermometry 2.0 was used to measure the availability of thermogenic capacity of brown adipocyte under various stimulations