AUTHOR=Yallampalli Chandra , Betancourt Ancizar , Mishra Akansha , Pennington Kathleen A. , Ruano Simone Hernandez , Tacam Moises , Chauhan Madhu 

TITLE=Role of adrenomedullin2/ intermedin in pregnancy induced vascular and metabolic adaptation in mice

JOURNAL=Frontiers in Physiology

VOLUME=Volume 14 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2023.1116042

DOI=10.3389/fphys.2023.1116042

ISSN=1664-042X

ABSTRACT=This study was designed to assess the role of Adrenomedullin2 (ADM2) in pregnancy induced vascular and metabolic adaptation using ADM2 knockout mice (Adm2-/-). The Adm2-/- mice	
were successfully generated using Clustered Regularly Interspaced Short Palindromic Repeats
(CRISPR) / Nuclease Cas 9 system. Current  data shows that:  1) Ablation of ADM2 does not affect the gestational length of mice; 2) Adm2-/-  females are fertile with no significant difference in number of pups/litter compared to Adm2+/+; 3) Total number of pups born dead or that die after birth is greater in Adm2-/- mice compared to Adm2+/+ mice (p=0.01);  4) Blood pressure in Adm2-/- mice is elevated during pregnancy compared to Adm2+/+ mice (p<0.05);5) The vascular sensitivity for CGRP and ADM mediated relaxation are unaltered in Adm2-/- mice compared to Adm2+/+ during pregnancy ; 6) The sensitivity of mesenteric artery for the contractile responses  to angiotensin 2 are  enhanced in Adm2-/-  mice compared to Adm2+/+(p<0.05) ;7)  Serum sFLT-1 levels are higher in pregnant Adm2-/- mice compared to pregnant Adm2+/+ mice (p=0.015),8) Adm2-/-  mice develop glucose intolerance during pregnancy compared to their wild type littermates with elevated serum levels of Insulin and triglycerides in Adm2-/-  mice compared to the wild types. Therefore, current data suggests a physiological role for ADM2 in pregnancy induced vascular and metabolic adaptations in mice.