AUTHOR=Driouich Azeddine , follet-gueye marie-laure , Bernard sophie , kousar sumaira , Chevalier Laurence , Vicré Maité , Lerouxel Olivier TITLE=Golgi-Mediated Synthesis and Secretion of Matrix Polysaccharides of the Primary Cell Wall of Higher Plants JOURNAL=Frontiers in Plant Science VOLUME=Volume 3 - 2012 YEAR=2012 URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2012.00079 DOI=10.3389/fpls.2012.00079 ISSN=1664-462X ABSTRACT=

The Golgi apparatus of eukaryotic cells is known for its central role in the processing, sorting, and transport of proteins to intra- and extra-cellular compartments. In plants, it has the additional task of assembling and exporting the non-cellulosic polysaccharides of the cell wall matrix including pectin and hemicelluloses, which are important for plant development and protection. In this review, we focus on the biosynthesis of complex polysaccharides of the primary cell wall of eudicotyledonous plants. We present and discuss the compartmental organization of the Golgi stacks with regards to complex polysaccharide assembly and secretion using immuno-electron microscopy and specific antibodies recognizing various sugar epitopes. We also discuss the significance of the recently identified Golgi-localized glycosyltransferases responsible for the biosynthesis of xyloglucan (XyG) and pectin.