AUTHOR=Peters Katrin , Belt Katharina , Braun Hans-Peter 

TITLE=3D Gel Map of Arabidopsis Complex I

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 4 - 2013

YEAR=2013

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2013.00153

DOI=10.3389/fpls.2013.00153

ISSN=1664-462X

ABSTRACT=<p>Complex I has a unique structure in plants and includes extra subunits. Here, we present a novel study to define its protein constituents. Mitochondria were isolated from <italic>Arabidopsis thaliana</italic> cell cultures, leaves, and roots. Subunits of complex I were resolved by 3D blue-native (BN)/SDS/SDS-PAGE and identified by mass spectrometry. Overall, 55 distinct proteins were found, seven of which occur in pairs of isoforms. We present evidence that <italic>Arabidopsis</italic> complex I consists of 49 distinct types of subunits, 40 of which represent homologs of bovine complex I. The nine other subunits represent special proteins absent in the animal linage of eukaryotes, most prominently a group of subunits related to bacterial gamma-type carbonic anhydrases. A GelMap <uri xlink:href="http://www.gelmap.de/arabidopsis-3d-complex-i/" xmlns:xlink="http://www.w3.org/1999/xlink">http://www.gelmap.de/arabidopsis-3d-complex-i/</uri> is presented for promoting future complex I research in <italic>Arabidopsis thaliana</italic>.</p>