AUTHOR=Fekecsová Sona , Danchenko Maksym , Uvackova Lubica , Skultety Ludovit , Hajduch Martin 

TITLE=Using 7 cm immobilized pH gradient strips to determine levels of clinically relevant proteins in wheat grain extracts

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 6 - 2015

YEAR=2015

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2015.00433

DOI=10.3389/fpls.2015.00433

ISSN=1664-462X

ABSTRACT=The aim of the work was to test relatively simple proteomics approach based on phenol extraction, two-dimensional electrophoresis (2-DE) in combination with 7cm immobilized pH gradient strips for the determination of clinically relevant proteins in wheat grain.    Using this approach, 157 2-DE spots were quantified in biological triplicate, out of which 53 were identified by MALDI-TOF/TOF mass spectrometry. Clinically relevant proteins associated with celiac disease, wheat dependent exercise induced anaphylaxis, baker´s asthma, and food allergy, were detected in 24 2-DE spots.  The comparison with recent quantitative study suggested that gel-based and gel-free proteomics approaches are complementary for the detection and quantification of clinically relevant proteins in wheat grain.