AUTHOR=Bartels Benjamin , Svatoš Aleš 

TITLE=Spatially resolved in vivo plant metabolomics by laser ablation-based mass spectrometry imaging (MSI) techniques: LDI-MSI and LAESI

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 6 - 2015

YEAR=2015

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2015.00471

DOI=10.3389/fpls.2015.00471

ISSN=1664-462X

ABSTRACT=This short review aims to summarize the current developments and applications of mass spectrometry-based methods for in situ profiling and imaging of plants with minimal or no sample pre-treatment or manipulation. Infrared-laser ablation electrospray ionization (LAESI) and UV-laser desorption/ionization (LDI) methods are reviewed. The underlying mechanisms of the ionization techniques - namely, laser ablation of biological samples and electrospray ionization - as well as variations of the LAESI ion source for specific targets of interest are described.