AUTHOR=Jensen Lea M., Kliebenstein Daniel J., Burow Meike 

TITLE=Investigation of the multifunctional gene AOP3 expands the regulatory network fine-tuning glucosinolate production in Arabidopsis

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 6 - 2015

YEAR=2015

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2015.00762

DOI=10.3389/fpls.2015.00762

ISSN=1664-462X

ABSTRACT=<p>Quantitative trait loci (QTL) mapping studies enable identification of loci that are part of regulatory networks controlling various phenotypes. Detailed investigations of genes within these loci are required to ultimately understand the function of individual genes and how they interact with other players in the network. In this study, we use transgenic plants in combination with natural variation to investigate the regulatory role of the <italic>AOP3</italic> gene found in <italic>GS-AOP</italic> locus previously suggested to contribute to the regulation of glucosinolate defense compounds. Phenotypic analysis and QTL mapping in F2 populations with different <italic>AOP3</italic> transgenes support that the enzymatic function and the <italic>AOP3</italic> RNA both play a significant role in controlling glucosinolate accumulation. Furthermore, we find different loci interacting with either the enzymatic activity or the RNA of <italic>AOP3</italic> and thereby extend the regulatory network controlling glucosinolate accumulation.</p>