AUTHOR=Krčková Zuzana , Brouzdová Jitka , Daněk Michal , Kocourková Daniela , Rainteau Dominique , Ruelland Eric , Valentová Olga , Pejchar Přemysl , Martinec Jan 

TITLE=Arabidopsis non-specific phospholipase C1: characterization and its involvement in response to heat stress

JOURNAL=Frontiers in Plant Science

VOLUME=6

YEAR=2015

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2015.00928

DOI=10.3389/fpls.2015.00928

ISSN=1664-462X

ABSTRACT=<p>The <italic>Arabidopsis</italic> non-specific phospholipase C<sup><xref ref-type="fn" rid="fn01">1</xref></sup> (NPC) protein family is encoded by the genes <italic>NPC1 – NPC6</italic>. It has been shown that NPC4 and NPC5 possess phospholipase C activity; NPC3 has lysophosphatidic acid phosphatase activity. NPC3, 4 and 5 play roles in the responses to hormones and abiotic stresses. NPC1, 2 and 6 has not been studied functionally yet. We found that <italic>Arabidopsis</italic> NPC1 expressed in <italic>Escherichia coli</italic> possesses phospholipase C activity <italic>in vitro</italic>. This protein was able to hydrolyse phosphatidylcholine to diacylglycerol. NPC1-green fluorescent protein was localized to secretory pathway compartments in <italic>Arabidopsis</italic> roots. In the knock out T-DNA insertion line NPC1 (<italic>npc1</italic>) basal thermotolerance was impaired compared with wild-type (WT); <italic>npc1</italic> exhibited significant decreases in survival rate and chlorophyll content at the seventh day after heat stress (HS). Conversely, plants overexpressing NPC1 (NPC1-OE) were more resistant to HS compared with WT. These findings suggest that NPC1 is involved in the plant response to heat.</p>