AUTHOR=Singh Amit P. , Dixit Garima , Kumar Amit , Mishra Seema , Singh Pradyumna K. , Dwivedi Sanjay , Trivedi Prabodh K. , Chakrabarty Debasis , Mallick Shekhar , Pandey Vivek , Dhankher Om P. , Tripathi Rudra D. 

TITLE=Nitric Oxide Alleviated Arsenic Toxicity by Modulation of Antioxidants and Thiol Metabolism in Rice (Oryza sativa L.)

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 6 - 2015

YEAR=2016

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2015.01272

DOI=10.3389/fpls.2015.01272

ISSN=1664-462X

ABSTRACT=<p>Nitric oxide (NO) is a gaseous signaling molecule and has a profound impact on plant growth and development. It is reported to serve as pro oxidant as well as antioxidant in plant system. In the present study, we evaluated the protective role of NO against arsenate (As<sup>V</sup>) toxicity in rice plants. As<sup>V</sup> exposure has hampered the plant growth, reduced the chlorophyll content, and enhanced the oxidative stress, while the exogenous NO supplementation has reverted these symptoms. NO supplementation has reduced the arsenic (As) accumulation in root as well as shoot. NO supplementation to As<sup>V</sup> exposed plants has reduced the gene expression level of <italic>OsLsi1</italic> and <italic>OsLsi2</italic>. As<sup>V</sup> stress significantly impacted thiol metabolism, it reduced GSH content and GSH/GSSG ratio, and enhanced the level of PCs. NO supplementation maintained the GSH/GSSG ratio and reduced the level of PCs. NO supplementation reverted As<sup>V</sup> induced iron deficiency in shoot and had significant impact of gene expression level of various iron transporters (<italic>OsYSL2, OsFRDL1, OsIRT1</italic>, and <italic>OsIRO2</italic>). Conclusively, exogenous application of NO could be advantageous against As<sup>V</sup> toxicity and could confer the tolerance to As<sup>V</sup> stress in rice.</p>