AUTHOR=Pertl-Obermeyer Heidi , Trentmann Oliver , Duscha Kerstin , Neuhaus H. Ekkehard , Schulze Waltraud X. 

TITLE=Quantitation of Vacuolar Sugar Transporter Abundance Changes Using QconCAT Synthtetic Peptides

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 7 - 2016

YEAR=2016

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2016.00411

DOI=10.3389/fpls.2016.00411

ISSN=1664-462X

ABSTRACT=Measurements of protein abundance changes are important for biological conclusions on protein-related processes such as activity or complex formation. Proteomic analyses in general are almost routine tasks in many laboratories. However, a precise and quantitative description of (absolute) protein abundance changes requires careful experimental design and high data quality. A vast choice of metabolic labeling and label-free quantitation protocols are available, but the trade-off between quantitative precision and proteome coverage of quantified proteins as well as missing value problems remain. Here, we provide an example of a targeted proteomic approach using artificial standard proteins consisting of concatenated peptides of interest (QconCAT) to specifically quantify abiotic stress-induced abundance changes in low abundant vacuolar transporters. An advantage of this approach lies in the reliable quantitation of a limited set of low-abundant target proteins throughout different conditions. We show that vacuolar ATPase AVP1, sugar transporters of the ERDL (early responsive to dehydration-like) family, and TMT2 (tonoplast monosaccharide transporter 2) are increased in abundance upon salt stress.