AUTHOR=Rast Anna , Rengstl Birgit , Heinz Steffen , Klingl Andreas , Nickelsen Jörg 

TITLE=The Role of Slr0151, a Tetratricopeptide Repeat Protein from Synechocystis sp. PCC 6803, during Photosystem II Assembly and Repair

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 7 - 2016

YEAR=2016

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2016.00605

DOI=10.3389/fpls.2016.00605

ISSN=1664-462X

ABSTRACT=<p>The assembly and repair of photosystem II (PSII) is facilitated by a variety of assembly factors. Among those, the tetratricopeptide repeat (TPR) protein Slr0151 from <italic>Synechocystis</italic> sp. PCC 6803 (hereafter <italic>Synechocystis</italic>) has previously been assigned a repair function under high light conditions (<xref ref-type="bibr" rid="B54">Yang et al., 2014</xref>). Here, we show that inactivation of <italic>slr0151</italic> affects thylakoid membrane ultrastructure even under normal light conditions. Moreover, the level and localization of Slr0151 are affected in a variety of PSII-related mutants. In particular, the data suggest a close functional relationship between Slr0151 and Sll0933, which interacts with Ycf48 during PSII assembly and is homologous to PAM68 in <italic>Arabidopsis thaliana</italic>. Immunofluorescence analysis revealed a punctate distribution of Slr0151 within several different membrane types in <italic>Synechocystis</italic> cells.</p>