AUTHOR=Jin Changyu , Li Dong , Gao Chenhao , Liu Kaige , Qi Shuanghui , Duan Shaowei , Li Zixiong , Gong Jingyun , Wang Jianjun , Hai Jiangbo , Chen Mingxun 

TITLE=Conserved Function of ACYL–ACYL CARRIER PROTEIN DESATURASE 5 on Seed Oil and Oleic Acid Biosynthesis between Arabidopsis thaliana and Brassica napus

JOURNAL=Frontiers in Plant Science

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2017.01319

DOI=10.3389/fpls.2017.01319

ISSN=1664-462X

ABSTRACT=<p>Previous studies have shown that several ACYL–ACYL CARRIER PROTEIN DESATURASE (AtAAD) members in <italic>Arabidopsis thaliana</italic> are responsible for oleic acid (C18:1) biosynthesis. Limited research has been conducted on another member, AtAAD5, and its paralog BnAAD5 in the closely related and commercially important plant, <italic>Brassica napus</italic>. Here, we found that <italic>AtAAD5</italic> was predominantly and exclusively expressed in developing embryos at the whole seed developmental stages. The <italic>aad5</italic> mutation caused a significant decrease in the amounts of oil and C18:1, and a considerable increase in the content of stearic acid (C18:0) in mature seeds, suggesting that AtAAD5 functioned as an important facilitator of seed oil biosynthesis. We also cloned the full-length coding sequence of <italic>BnAAD5-1</italic> from the A3 subgenome of the <italic>B. napus</italic> inbred line L111. We showed that ectopic expression of <italic>BnAAD5-1</italic> in the <italic>A. thaliana aad5-2</italic> mutant fully complemented the phenotypes of the mutant, such as lower oil content and altered contents of C18:0 and C18:1. These results help us to better understand the functions of AAD members in <italic>A. thaliana</italic> and <italic>B. napus</italic> and provide a promising target for genetic manipulation of <italic>B. napus</italic>.</p>