AUTHOR=Deng Zhiying , Cui Yong , Han Qingdian , Fang Wenqi , Li Jifa , Tian Jichun 

TITLE=Discovery of Consistent QTLs of Wheat Spike-Related Traits under Nitrogen Treatment at Different Development Stages

JOURNAL=Frontiers in Plant Science

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2017.02120

DOI=10.3389/fpls.2017.02120

ISSN=1664-462X

ABSTRACT=<p>Spike-related traits such as spike length (Sl), fertile spikelet number (Fsn), sterile spikelet number (Ssn), grain number per spike (Gns), and thousand-kernel weight (Tkw) are important factors influencing wheat yield. However, reliably stable markers that can be used for molecular breeding in different environments have not yet been identified. In this study, a double haploid (DH) population was used for quantitative trait locus (QTL) mapping of five spike-related traits under four different nitrogen (N) supply dates in two locations and years. Seventy additive QTLs with phenotypic variation ranging from 4.12 to 34.74% and 10 major epistatic QTLs were identified. Eight important chromosomal regions on five chromosomes (1B, 2B, 2D, 5D, and 6A) were found. Sixteen stable QTLs were detected for which N application had little effect. Among those stable QTLs, <italic>QSl.sdau-2D-1</italic>, and <italic>QSl.sdau-2D-2</italic>, with phenotypic variation explained (PVE) of 10.4 and 24.2%, respectively, were flanked by markers <italic>Xwmc112</italic> and <italic>Xcfd53</italic> in the same order. The QTLs <italic>QSsn.sdau-2B-1, QFsn.sdau-2B-1</italic>, and <italic>QGns.sdau-2B</italic>, with PVE ranging from 4.37 to 28.43%, collocated in the <italic>Xwmc179</italic>-<italic>Xbarc373</italic> marker interval. The consistent kernel wheat QTL (<italic>QTkw.sdau-6A</italic>) on the long arm of chromosome 6A, flanked by SSR markers <italic>Xbarc1055</italic> and <italic>Xwmc553</italic>, showed PVE of 5.87–15.18%. Among these stable QTLs, the two flanking markers <italic>Xwmc112</italic> and <italic>Xcfd53</italic> have been validated using different varieties and populations for selecting Sl. Therefore, these results will be of great value for marker-assisted selection (MAS) in breeding programs and will accelerate the understanding of the genetic relationships among spike-related traits at the molecular level.</p>