AUTHOR=Qian Lichao , Zhao Jinping , Du Yumei , Zhao Xijuan , Han Meng , Liu Yule 

TITLE=Hsp90 Interacts With Tm-22 and Is Essential for Tm-22-Mediated Resistance to Tobacco mosaic virus

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 9 - 2018

YEAR=2018

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2018.00411

DOI=10.3389/fpls.2018.00411

ISSN=1664-462X

ABSTRACT=<p>The tomato resistance gene <italic>Tm-2</italic><sup>2</sup> encodes a coiled coil-nucleotide binding site-leucine rich repeat type resistance protein and confers effective immune response against tobamoviruses by detecting the presence of viral movement proteins (MPs). In this study, we show that the <italic>Nicotiana benthamiana</italic> Heat shock protein 90-kD (Hsp90) interacts with Tm-2<sup>2</sup>. Silencing of <italic>Hsp90</italic> reduced <italic>Tm-2</italic><sup>2</sup>-mediated resistance to <italic>Tobacco mosaic virus</italic> (TMV) and the steady-state levels of Tm-2<sup>2</sup> protein. Further, Hsp90 associates with SGT1 in yeast and in plant cells. These results suggest that Hsp90-SGT1 complex takes part in <italic>Tm-2</italic><sup>2</sup>-mediated TMV resistance by functioning as chaperone to regulate Tm-2<sup>2</sup> stability.</p>