AUTHOR=Zhao Qingsong , Tong Ya , Yang Chunyan , Yang Yongqing , Zhang Mengchen 

TITLE=Identification and Mapping of a New Soybean Male-Sterile Gene, mst-M

JOURNAL=Frontiers in Plant Science

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.00094

DOI=10.3389/fpls.2019.00094

ISSN=1664-462X

ABSTRACT=<p>The use of sterility is common in plants and multiple loci for hybrid sterility have been identified in crops such as rice. In soybean, fine-mapping and research on the molecular mechanism of male sterility is limited. Here, we identified a male-sterile soybean line, which produces larger, abnormal pollen grains that stain poorly with I<sub>2</sub>-KI. In an inheritance test, all <italic>F</italic><sub>1</sub> plants were fertile and the <italic>F</italic><sub>2</sub> and <italic>F</italic><sub>2:3</sub> populations conformed with the expected segregation ratio of 3:1 (fertility:sterility) (<italic>p</italic> = 0.82) and showed a 1:2:0 ratio of homozygous fertile: heterozygous fertile: homozygous sterile genotypes (<italic>p</italic> = 0.73), suggesting that the sterility was controlled by a single recessive gene (designated “<italic>mst-M</italic>”). Bulked segregant analysis showed that almost all single-nucleotide polymorphisms (SNPs; 95.92%) were distributed on chromosome 13 and 868 SNPs (95.81%) were distributed in the physical region of Chromosome 13.21877872 to Chromosome 13.22862641. Genetic mapping revealed that <italic>mst-M</italic> was flanked by <italic>W1</italic> and dCAPS-1 with genetic distances of 0.6 and 1.8 cM, respectively. The order of the consensus markers and known sterility genes was: Satt146 – (5.0 cM) – <italic>st5</italic> – (2.5 cM) – Satt030 – (15.3 cM) – <italic>ms6</italic> – (5.0 cM) – Satt149 – (39.5 cM) – <italic>W1</italic> – (0.6 cM) – <italic><underline>mst-M</underline></italic> – (14.1 cM) – Satt516 (7.5 cM) – <italic>ms1</italic> – (16.3 cM) – Satt595. These results suggest that <italic>mst-M</italic> is a newly identified male-sterility gene, which represents an alternative genetic resource for developing a hybrid seed production system for soybean.</p>