AUTHOR=Pizarro Lorena , Leibman-Markus Meirav , Schuster Silvia , Bar Maya , Avni Adi TITLE=Tomato Dynamin Related Protein 2A Associates With LeEIX2 and Enhances PRR Mediated Defense by Modulating Receptor Trafficking JOURNAL=Frontiers in Plant Science VOLUME=10 YEAR=2019 URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.00936 DOI=10.3389/fpls.2019.00936 ISSN=1664-462X ABSTRACT=

The endocytic trafficking pathway is employed by the plant to regulate immune responses, and is often targeted by pathogen effectors to promote virulence. The model system of the tomato receptor-like protein (RLP) LeEIX2 and its ligand, the elicitor EIX, employs endocytosis to transmit receptor-mediated signals, with some of the signaling events occurring directly from endosomal compartments. Here, to explore the trafficking mechanism of LeEIX2-mediated immune signaling, we used a proteomic approach to identify LeEIX2-associating proteins. We report the identification of SlDRP2A, a dynamin related protein, as an associating partner for LeEIX2. SlDRP2A localizes at the plasma membrane. Overexpression of SlDRP2A increases the sub-population of LeEIX2 in VHAa1 endosomes, and enhances LeEIX2- and FLS2-mediated defense. The effect of SlDRP2A on induction of plant immunity highlights the importance of endomembrane components and endocytosis in signal propagation during plant immune responses.