AUTHOR=Ramalingam Jegadeesan , Raveendra Chandavarapu , Savitha Palanisamy , Vidya Venugopal , Chaithra Thammannagowda Lingapatna , Velprabakaran Senthilvel , Saraswathi Ramasamy , Ramanathan Ayyasamy , Arumugam Pillai Madhavan Pillai , Arumugachamy Samudrakani , Vanniarajan Chockalingam 

TITLE=Gene Pyramiding for Achieving Enhanced Resistance to Bacterial Blight, Blast, and Sheath Blight Diseases in Rice

JOURNAL=Frontiers in Plant Science

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.591457

DOI=10.3389/fpls.2020.591457

ISSN=1664-462X

ABSTRACT=<p>Bacterial blight, blast, and sheath blight are the commonest diseases causing substantial yield loss in rice around the world. Stacking of broad-spectrum resistance genes/QTLs into popular cultivars is becoming a major objective of any disease resistance breeding program. The varieties ASD 16 and ADT 43 are the two popular, high yielding, and widely grown rice cultivars of South India, which are susceptible to bacterial blight (BB), blast, and sheath blight diseases. The present study was carried out to improve the cultivars (ASD 16 and ADT 43) through introgression of bacterial blight (<italic>xa5</italic>, <italic>xa13</italic>, and <italic>Xa21</italic>), blast (<italic>Pi54</italic>), and sheath blight (<italic>qSBR7-1</italic>, <italic>qSBR11-1</italic>, and <italic>qSBR11-2</italic>) resistance genes/QTLs by MABB (marker-assisted backcross breeding). IRBB60 (<italic>xa5</italic>, <italic>xa13</italic>, and <italic>Xa21</italic>) and Tetep (<italic>Pi54</italic>; <italic>qSBR7-1</italic>, <italic>qSBR11-1</italic>, and <italic>qSBR11-2</italic>) were used as donors to introgress BB, blast, and sheath blight resistance into the recurrent parents (ASD 16 and ADT 43). Homozygous (BC<sub>3</sub>F<sub>3</sub> generation), three-gene bacterial blight pyramided (<italic>xa5</italic> + <italic>xa13</italic> + <italic>Xa21</italic>) lines were developed, and these lines were crossed with Tetep to combine blast (<italic>Pi54</italic>) and sheath blight (<italic>qSBR7-1</italic>, <italic>qSBR11-1</italic>, and <italic>qSBR11-2</italic>) resistance. In BC<sub>3</sub>F<sub>3</sub> generation, the improved pyramided lines carrying a total of seven genes/QTLs (<italic>xa5</italic> + <italic>xa13</italic> + <italic>Xa21</italic> + <italic>Pi54</italic> + <italic>qSBR7-1</italic> + <italic>qSBR11-1</italic> + <italic>qSBR11-2</italic>) were selected through molecular and phenotypic assay, and these were evaluated for resistance against bacterial blight, blast, and sheath blight pathogens under greenhouse conditions. We have selected nine lines in ASD 16 background and 15 lines in ADT 43 background, exhibiting a high degree of resistance to BB, blast, and sheath blight diseases and also possessing phenotypes of recurrent parents. The improved pyramided lines are expected to be used as improved varieties or used as a potential donor in breeding programs. The present study successfully introgressed <italic>Pi54</italic>, and <italic>qSBR</italic> QTLs (<italic>qSBR7-1</italic>, <italic>qSBR11-1</italic>, and <italic>qSBR11-2</italic>) from Tetep and major effective BB-resistant genes (<italic>xa5</italic>, <italic>xa13</italic>, and <italic>Xa21</italic>) from IRBB60 into the commercial varieties for durable resistance to multiple diseases.</p>