AUTHOR=Zhang Bo , Yang Hui-Juan , Yang Ya-Zhou , Zhu Zhen-Zhen , Li Ya-Nan , Qu Dong , Zhao Zheng-Yang 

TITLE=mdm-miR828 Participates in the Feedback Loop to Regulate Anthocyanin Accumulation in Apple Peel

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 11 - 2020

YEAR=2020

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.608109

DOI=10.3389/fpls.2020.608109

ISSN=1664-462X

ABSTRACT=Anthocyanins are responsible for the red pigmentation in the peel of apple (Malus × domestica Borkh.) fruit. Relatively few studies have investigated anthocyanins at the post-transcriptional level. MicroRNAs play an important role in plant growth and development by regulating gene expression at the post-transcriptional level. In this study, mdm-miR828 showed a relatively low expression level during the rapid fruit coloration period. However, the mdm-miR828 expression level increased in the late fruit coloration stage. Overexpression of mdm-miR828 inhibited anthocyanin synthesis in apple and Arabidopsis. Dual-luciferase and yeast one-hybrid assays showed that MdMYB1 is capable of binding to the promoter of mdm-MIR828b to promote its expression. The results indicate that mdm-miR828 is involved in a feedback regulatory mechanism associated with anthocyanin accumulation in apple. In addition, mdm-miR828 may be involved in the inhibition of anthocyanin accumulation in response to high temperature.