AUTHOR=Fang Yunxia , Zhang Xiaoqin , Zhang Xian , Tong Tao , Zhang Ziling , Wu Gengwei , Hou Linlin , Zheng Junjun , Niu Chunyu , Li Jia , Wang Wenjia , Wang Hua , Xue Dawei 

TITLE=A High-Density Genetic Linkage Map of SLAFs and QTL Analysis of Grain Size and Weight in Barley (Hordeum vulgare L.)

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 11 - 2020

YEAR=2020

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.620922

DOI=10.3389/fpls.2020.620922

ISSN=1664-462X

ABSTRACT= Grain size is an important agronomic trait determines yield in barley, and a high-density genetic map is helpful to accurately detect quantitative trait loci (QTLs) related to grain traits. Using SLAF-seq technology, a high-density genetic map was constructed with a population of 134 recombinant inbred lines (RILs) deriving from a cross between Golden Promise (GP) and H602, which contained 12,635 SLAFs with 26,693 SNPs, and spanned 896.74 cM with an average interval of 0.07 cM on 7 chromosomes. Based on the map, a total of 16 QTLs for grain length, grain width and thousand-grain weight were detected on 1H, 2H, 4H, 5H and 6H. Among them, a major QTL locus qGL1, accounting for the max phenotypic variance of 16.7% was located on 1H, which is a new unreported QTL affecting grain length. In addition, the other two QTLs, qGL5 and qTGW5, accounting for the max phenotypic variances of 20.7% and 21.1% respectively, were identified in the same region, and sequencing results showed they are identical to HvDep1 gene. These results indicate that it is a feasible approach to construct a high-quality genetic map for QTL mapping by using SLAF markers, and the detected major QTLs qGL1, qGL5 and qTGW5 are useful for marker- assisted selection (MAS) of grain size in barley breeding.