AUTHOR=Zhao Xuehui , Wen Binbin , Li Chen , Tan Qiuping , Liu Li , Chen Xiude , Li Ling , Fu Xiling 

TITLE=Overexpression of the Peach Transcription Factor Early Bud-Break 1 Leads to More Branches in Poplar

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 12 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.681283

DOI=10.3389/fpls.2021.681283

ISSN=1664-462X

ABSTRACT=Shoot branching is an important adaptive trait that determines plant architecture. In our previous study, the EBB1 gene in peach (Prunus persica var. nectarina) cultivar Zhongyou 4 was transformed into poplar (Populus trichocarpa). PpEBB1-oe poplar showed more branched phenotype. To understand the potential mechanisms underlying EBB1-mediated branching, transcriptomic and proteomics analyses were used. The results showed that a large number of DEGs/DEPs associated with light response, sugars, brassinosteroids and nitrogen metabolism were significantly enriched in PpEBB1-oe poplar. In addition, contents of sugars, brassinosteroids and amino acids were measured. Results showed that PpEBB1 significantly promoted the accumulation of fructose, glucose, sucrose, trehalose and starch. Contents of BL, CS and 6-deoxoCS were all significantly changed with overexpressing PpEBB1. Various types of amino acids were measured and four of them were significantly improved in PpEBB1-oe poplar, including Asp, Arg, Cys and Trp. Taken together, shoot branching is a process controlled by a complex regulatory network, and PpEBB1 may play important roles in this process through the coordinating multiple metabolic pathways involved in shoot branching including light reponse, phytohormones, sugars, and nitrogen.