AUTHOR=Ma Li , Jiang Hong , Bi Yang , Li Yong-Cai , Yang Jiang-Wei , Si Huai-Jun , Ren Ying-Yue , Prusky Dov 

TITLE=The Interaction Between StCDPK14 and StRbohB Contributes to Benzo-(1, 2, 3)-Thiadiazole-7-Carbothioic Acid S-Methyl Ester-Induced Wound Healing of Potato Tubers by Regulating Reactive Oxygen Species Generation

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 12 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.737524

DOI=10.3389/fpls.2021.737524

ISSN=1664-462X

ABSTRACT=Reactive oxygen species (ROS) production is essential for both physiological processes and environmental stress in diverse plants. Previous studies found BTH-inducible ROS were associated with wound healing of potato tubers. Calcium-dependent protein kinases (CDPKs), the important calcium receptors, are known to play a crucial part in plant development and adaptation to abiotic stresses. However, whether CDPK-mediated ROS generation induced by BTH involved in wound healing is elusive. Here, we measured StCDPKs expression using real-time PCR, it was found that the transcriptional levels of StCDPKs from BTH-treated tissues were significantly induced, among which StCDPK14 presented the most increased level. Subcellular localization results showed that StCDPK14 is located in the nucleus and membrane. The transgenic potato plants and tubers were developed using interference-expression of StCDPK14 by Agrobacterium tumefaciens–mediated transformation. The StRbohs expression showed a remarkable decrease in StCDPK14 transgenic tubers, notably, H2O2 content and suberin deposition were also significantly declined. To confirm the relationship between StCDPK14 and StRbohB, yeast two-hybrid and bimolecular fluorescence complementation were used to examine the interaction, it was shown that StCDPK14 interacted with EF-hand motifs of StRbohB N-terminus. These results above unraveled that StCDPK14 functions in ROS generation via interacting with StRbohB during wound-healing of potato tubers.