AUTHOR=Ma Li , Jiang Hong , Bi Yang , Li Yong-Cai , Yang Jiang-Wei , Si Huai-Jun , Ren Ying-Yue , Prusky Dov 

TITLE=The Interaction Between StCDPK14 and StRbohB Contributes to Benzo-(1, 2, 3)-Thiadiazole-7-Carbothioic Acid S-Methyl Ester-Induced Wound Healing of Potato Tubers by Regulating Reactive Oxygen Species Generation

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 12 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.737524

DOI=10.3389/fpls.2021.737524

ISSN=1664-462X

ABSTRACT=<p>Reactive oxygen species (ROS) production is essential for both physiological processes and environmental stress in diverse plants. Previous studies have found that benzo-(1, 2, 3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH)-inducible ROS were associated with wound healing of potato tubers. Calcium-dependent protein kinases (CDPKs), the important calcium receptors, are known to play a crucial part in plant development and adaptation to abiotic stresses. However, whether CDPK-mediated ROS generation induced by BTH is involved in wound healing is elusive. In this study, we measured <italic>Solanum tuberosum CDPKs</italic> (<italic>StCDPKs</italic>) expression using real-time PCR, and it was found that the transcriptional levels of <italic>StCDPKs</italic> from BTH-treated tissues were significantly induced, among which <italic>StCDPK14</italic> presented the most increased level. Subcellular localization results showed that StCDPK14 is located in the nucleus and membrane. The transgenic potato plants and tubers were developed using interference-expression of <italic>StCDPK14</italic> by <italic>Agrobacterium tumefaciens</italic>–mediated transformation. The <italic>St</italic> respiratory burst oxidase homologs (<italic>StRbohs</italic>) expression showed a remarkable decrease in <italic>StCDPK14</italic> transgenic tubers, notably, H<sub>2</sub>O<sub>2</sub> content and suberin deposition were also significantly declined. To confirm the relationship between StCDPK14 and StRbohB, yeast-two-hybrid and bimolecular fluorescence complementation were used to examine the interaction, and it was shown that StCDPK14 interacted with the specific Ca<sup>2 +</sup> -binding motif (helix-loop-helix, called EF-hand) of StRbohB N-terminus. The above results unraveled that StCDPK14 functions in ROS generation <italic>via</italic> interacting with StRbohB during wound healing of potato tubers.</p>