AUTHOR=Qiao Ling , Li Hanlin , Wang Jie , Zhao Jiajia , Zheng Xingwei , Wu Bangbang , Du Weijun , Wang Juanling , Zheng Jun 

TITLE=Analysis of Genetic Regions Related to Field Grain Number per Spike From Chinese Wheat Founder Parent Linfen 5064

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 12 - 2021

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.808136

DOI=10.3389/fpls.2021.808136

ISSN=1664-462X

ABSTRACT=Wheat founder parents have been important in the development of new and more productive wheat varieties. Understanding the effects of specific genome regions on yield-related traits in founder variety derivatives can enable more efficient use of these genetic resources through molecular breeding. In the present study, the genetic regions related to field grain number per spike from the founder parent Linfen 5064 were analyzed using a haploid (DH) population developed from a cross between Linfen 5064 and Nongda 3338. QTL for five spike related traits over nine experimental locations/years were identified, including total spikelet number per spike (TSS), base sterile spikelet number per spike (BSSS), top sterile spikelet number per spike (TSSS), fertile spikelet number per spike (FSS), and grain number per spike (GNS). Thirteen stable QTL explaining 3.91-19.51% of the phenotypic variation were found. The effect of six of these QTL, Qtss.saw-2B.1, Qtss.saw-2B.2, Qtss.saw-3B, Qfss.saw-2B.2, Qbsss.saw-5A.1, and Qgns.saw-1A, were verified by another DH population (Linfen 5064 / Jinmai 47), which showed extreme significance (P<0.05) in more than three environments. No homologues of reported grain number related genes from grass species were found in the physical regions of Qtss.saw-2B.1 and Qtss.saw-3B, indicating both of them are novel QTL, or possess novel related genes. The positive alleles of Qtss.saw-2B.2 from Linfen 5064 has the lager effect on TSS (3.30%, 0.62) and has 66.89% percent in Chinese landmark cultivars under selecting. This study revealed three key regions for GNS in Linfen 5064 and provides insights into molecular marker assisted breeding.