AUTHOR=Liu Xintong , Fang Peihong , Wang Zicheng , Cao Xiaoqian , Yu Zhiyi , Chen Xi , Zhang Zhao 

TITLE=Comparative RNA-seq analysis reveals a critical role for ethylene in rose (Rosa hybrida) susceptible response to Podosphera pannosa

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.1018427

DOI=10.3389/fpls.2022.1018427

ISSN=1664-462X

ABSTRACT=Rose is one of the most important ornamental flowers, accounting for approximately one-third of the world's cut flower market. Powdery mildew caused by Podosphera pannosa is a devastating fungal diseases in rose, mainly infecting the young leaves, causing serious economic losses. Therefore, study on the mechanism of the fungus infecting rose leaves, and the possibility to improve resistance hereby, is interesting and meaningful. Accordingly, we conducted a transcriptome sequencing of rose leaves infected by P. pannosa at different time points to reveal the molecular mechanism of resistance to powdery mildew. The high-quality reads were aligned to reference genome of Rosa chinensis, yielding 51230 transcripts. A total of 1181 differentially expressed genes were identified in leaves during P. pannosa infection at 12 hpi, 24 hpi and 48 hpi. The transcription factors of ERF, MYB, bHLH, WRKY, etc. family were identified among DEGs and most of them were down-regulated during P. pannosa infection. KEGG analysis showed that hormone signal transduction pathway, especially ethylene signal related genes, were consistently down-regulated expression during powdery mildew infection. More importantly, exogenous 1-MCP (inhibitor of ET) treatment could improve rose leaves resistance to P. pannosa. Summarily, our transcriptome of rose leaf infected by powdery mildew gives universal insights into the complex gene regulatory networks mediating the rose leaf response to P. pannosa. Further demonstrated the positive role of 1-MCP in resistance to biotrophic pathogens.