AUTHOR=Wu Yu , Liu Huimin , Bing Jie , Zhang Genfa 

TITLE=Integrative transcriptomic and TMT-based proteomic analysis reveals the mechanism by which AtENO2 affects seed germination under salt stress

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.1035750

DOI=10.3389/fpls.2022.1035750

ISSN=1664-462X

ABSTRACT=Seed germination is critical for plant survival and agricultural production and is affected by many cues, including internal factors and external environmental conditions. As a key enzyme in glycolysis, enolase 2 (ENO2) also plays a vital role in plant growth and abiotic stress responses. In our research, we found that the seed germination rate was lower in the AtENO2 mutation (eno2-) than in the wild type (WT) under salt stress in Arabidopsis thaliana, while there was no significant difference under normal conditions. However, our knowledge of the mechanisms by which AtENO2 regulates seed germination under salt stress remains limited. In the current study, transcriptome and proteome analyses were used to compare eno2- and the WT under normal and salt stress conditions at the germination stage. There were 417 (208 upregulated and 209 downregulated) and 4442 (2032 upregulated and 2410 downregulated) differentially expressed genes (DEGs) identified by transcriptome, and 302 (120 upregulated and 182 downregulated) and 1929 (761 upregulated and 1168 downregulated) differentially expressed proteins (DEPs) qualified by proteome under normal and salt stress conditions, respectively. The combined analysis found abundant DEGs and DEPs related to stresses (PIP1B, GAPA1, GAPB, etc.) and hydrogen peroxide removal (APXT, APX4, etc.) were highly downregulated in eno2-. In addition, several DEGs and DEPs encoding phytohormone transduction pathways, i.e., auxin (PIN4 and AUX1) and GA (GASA1), were identified and may have a vital role in the contribution to salt tolerance in seed germination under AtENO2 deficiency. The DEGs or DEPs related to ABA signaling (PED1, ACO3, HY5, etc.) were relatively greatly upregulated in eno2- under salt stress. Moreover, we constructed an interactive network and further identified GAPA1 and GAPB that could interact with AtENO2, which may explain the function of AtENO2 under salt stress during seed germination. Overall, our study lays the foundation for further exploration of the molecular function of AtENO2 under salt stress at the seed germination stage in Arabidopsis thaliana.