AUTHOR=Lan Zhaohui , Shi Yuhua , Yin Qinggang , Gao Ranran , Liu Chunlian , Wang Wenting , Tian Xufang , Liu Jiawei , Nong Yiying , Xiang Li , Wu Lan TITLE=Comparative and phylogenetic analysis of complete chloroplast genomes from five Artemisia species JOURNAL=Frontiers in Plant Science VOLUME=Volume 13 - 2022 YEAR=2022 URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.1049209 DOI=10.3389/fpls.2022.1049209 ISSN=1664-462X ABSTRACT=Artemisia Linn. is a large genus within the family Asteraceae that includes several important medicinal plants. Most Artemisia species contain terpenoids and flavonoids, which are commonly used in antimalarial, antiinflammatory, hemostatic, and choleretic drugs. Because of their similar morphology and chemical composition, traditional identification methods often fail to distinguish them, which is not conducive for their circulation and drug safety. Therefore, developing an effective identification method for Artemisia species is an urgent requirement. In this study, we analyzed 15 chloroplast (cp) genomes, including 12 newly sequenced genomes, from 5 Artemisia species. We explored their genomic features, codon usage, amino acid frequency, simple sequence repeats (SSRs), and nucleotide diversity analyses. Finally, we constructed a phylogenetic tree comprising 29 cp genomes using maximum likelihood methods. The cp genomes from the five Artemisia species had a typical quadripartite structure and were highly conserved across species. They had varying lengths of 151,132–151,178 bp, and their gene content and codon preferences were similar. A total of 132 genes were annotated, of which 114 were unique and contained 80 protein-coding genes, 30 tRNAs, and 4 rRNAs. We observed 42–50 long repeats and 188–193 SSRs in the cp genomes. Mutation hotspot analysis identified four highly variable regions, which can potentially be used as molecular markers to identify Artemisia species. Phylogenetic analysis showed that the five Artemisia species investigated in this study were sister branches to each other, and individuals of each species formed a monophyletic clade. This study shows that the cp genome can provide distinguishing features to help identify closely related Artemisia species and has the potential to serve as a universal super barcode for plant identification.