AUTHOR=Yang Ying , Wang Jiayan , Xu Yan , Abbas Farhat , Xu Dongbin , Tao Shenchen , Xie Xiaoting , Song Feng , Huang Qiaoyu , Sharma Anket , Zheng Luqing , Yan Daoliang , Wang Xiaofei , Zheng Bingsong , Yuan Huwei , Wu Rongling , He Yi 

TITLE=Genome-wide identification and expression analysis of AUX/LAX family genes in Chinese hickory (Carya cathayensis Sarg.) Under various abiotic stresses and grafting

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 13 - 2022

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.1060965

DOI=10.3389/fpls.2022.1060965

ISSN=1664-462X

ABSTRACT=Auxin is essential for regulating plant growth and development, as well as plant response to abiotic stresses. The auxin influx transporter AUX/LAX protein belongs to the amino acid permease family of proton-driven transporters and is involved in the transport of indole-3-acetic acid (IAA). For the first time, eight cDNAs encoding the open reading frame of the Chinese hickory AUX/LAX family were identified and characterized. The family proteins were highly conserved. Evolutionary analysis showed that CcAUX/LAXs were more closely related to their homologous proteins in woody plants. Tissue-specific expression analysis of the CcAUX/LAX genes revealed that expression of this family members was higher in the root and lower in the stem. The promoters of CcAUX/LAX genes contained a large number of light responsive, hormone responsive, and abiotic stress responsive cis-regulatory elements. CcAUX/LAX genes responded differently to different levels of drought and salt stress treatments. Furthermore, expression analysis revealed that CcAUX/LAX genes played complex regulatory roles during Chinese hickory grafting. These findings will not only be regarded as a valuable resource for further functional validation of CcAUX/LAXs, but also contribute to a better understanding of the molecular basis for stress regulation by auxin in Chinese hickory.