AUTHOR=Wu Yanqing , Liu Jiao , Zhou Guisheng 

TITLE=Whole-transcriptome analyses of Sorghum leaves identify key mRNAs and ncRNAs associated with GA3-mediated alleviation of salt stress

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.1071657

DOI=10.3389/fpls.2022.1071657

ISSN=1664-462X

ABSTRACT=Sorghum has attracted much attention in recent years for its tolerance in high salt environments. However, the effect and regulatory mechanism of the gibberellic acid (GA3)-mediated alleviation of salt stress in sorghum remains unclear. Herein, we reported that a GA3 concentrations of 50 mg/L is optimal for sorghum (“Jitian 3”) development under salt stress. We conducted a whole-transcriptome analysis between GA3-treated and control sorghum leaves under salt stress, and we identified 1002 differentially expressed (DE)-mRNAs, 81 DE-lncRNAs, 7 DE-circRNAs, and 26 DE-miRNAs in sorghum following GA3 treatment. We also identified a majority of DE-mRNAs and DE-ncRNAs targets that serve essential roles in phenylpropanoid biosynthesis and plant hormone networks. In addition, we generated a ceRNA-miRNA-target gene network, and 3 circRNAs (circRNA_2746, circRNA_6515, circRNA_5622) and 4 lncRNAs (XR_002450182.1, XR_002452422.1, XR_002448510.1, XR_002448296.1) probably acts as valuable candidates for the regulation of the GA3-mediated alleviation of salt stress in sorghum. Our findings uncovered potential mRNA and non-coding RNAs that contribute to GA3 regulation, thus offering a basis for the future investigation of underlying mechanisms of salt stress in sorghum.