AUTHOR=Zhang Liqing , Li Shuigen , Fang Xianping , An Haishan , Zhang Xueying 

TITLE=Genome-wide analysis of LysM gene family members and their expression in response to Colletotrichum fructicola infection in Octoploid strawberry(Fragaria × ananassa)

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.1105591

DOI=10.3389/fpls.2022.1105591

ISSN=1664-462X

ABSTRACT=<p>The cultivated octoploid strawberry (<italic>Fragaria</italic> × <italic>ananassa</italic>) is an economically important fruit that is planted worldwide. The lysin motif (LysM) protein family is composed of the major class of plant pattern recognition receptors, which play important roles in sensing pathogen-associated molecular patterns (PAMPs), and subsequently triggers downstream plant immunity. In the present study, a comprehensive, genome-wide analysis of <italic>F</italic>. × <italic>ananassa LysM</italic> (<italic>FaLysM</italic>) genes was performed to investigate gene structures, phylogenic relationships, chromosome location, collinear relationships, transcription factor binding sites, and protein model analysis. We aimed to identify the <italic>LysM</italic> genes involved in the defense against plant pathogens. A total of 14 <italic>FaLysM</italic> genes were identified in the <italic>F</italic>. × <italic>ananassa</italic> genome and divided into 2 subgroups (LYP and LYK) on the basis of the phylogenetic analysis. The Ka/Ks ratio for the duplicated pair of most <italic>FaLysM</italic> genes was less than 1, which indicates that the selection pressure was mostly subject to the purifying selection during evolution. The protein model analysis revealed that FaLysM2-10 contain conserved mode of chitin binding, which suggest the potential role of FaLysM2-10 in pathogen perception and plant immunity. The RNA-Seq results showed the differential regulation of 14 <italic>FaLysM</italic> genes in response to <italic>Colletotrichum fructicola</italic> infection, implying the complex interaction between <italic>C</italic>. <italic>fructicola</italic> and strawberry. Knockout of candidate effector gene <italic>CfLysM2</italic>, which was previously proved to be highly expressed during <italic>C</italic>. <italic>fructicola</italic> infection, resulted in the up-regulation of six <italic>FaLysM</italic> genes (<italic>FaLysM1</italic>, <italic>FaLysM2</italic>, <italic>FaLysM3</italic>, <italic>FaLysM7</italic>, <italic>FaLysM8</italic>, and <italic>FaLysM12</italic>), indicating the competitive relations between <italic>CfLysM2</italic> and <italic>FaLysM</italic> genes. Overall, this study provides fundamental information on the roles of LysM proteins in octoploid strawberry and its interaction with <italic>C</italic>. <italic>fructicola</italic>, laying useful information for further investigation on the <italic>C</italic>. <italic>fructicola</italic>-strawberry interaction and strawberry resistance breeding.</p>