AUTHOR=Hou Siyu , Ren Xuemei , Yang Yang , Wang Donghang , Du Wei , Wang Xinfang , Li Hongying , Han Yuanhuai , Liu Longlong , Sun Zhaoxia 

TITLE=Genome-Wide Development of Polymorphic Microsatellite Markers and Association Analysis of Major Agronomic Traits in Core Germplasm Resources of Tartary Buckwheat

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.819008

DOI=10.3389/fpls.2022.819008

ISSN=1664-462X

ABSTRACT=Tartary buckwheat (Fagopyrum tataricum Gaertn.) is an important multigrain crop and medicinal plant, but its functional genomics and molecular breeding research have been lagging for a long time. Here, a genome-wide screening was performed to develop SSR markers and associated with 7 major agronomic traits and rutin content of 97 core germplasms. A total of 40,901 SSR loci were found and uniformly distributed across the whole tartary buckwheat genome with a mean distance of 11 Kb between each other. Among them, 8,089 pairs of SSR primers were designed and 101 paired of polymorphic primers were used for genotyping of 97 core germplasms. These polymorphic loci showed a high genetic variation in these core germplasms with an average of 0.476. In addition, fv, such as SXAU1108 (100-grain weight), SXAU7142 (plant height), and SXAU3257 (number of main stem nodes). Finally, based on gene function annotation and homology analysis, a candidate gene FtPinG0007685500 that may affect the node number and stem diameter of the main stem by participating in the lignin synthesis was found. This study reported the genome-wide SSR loci mining and marker development on tartary buckwheat, all SSR loci will help the genotyping of tartary buckwheat germplasms. Additionally, the detected markers and candidate genes could be further used for marker-assisted breeding and functional gene cloning of tartary buckwheat.