AUTHOR=Sun Chao , Liang Wenjun , Yan Kan , Xu Derong , Qin Tianyuan , Fiaz Sajid , Kear Philip , Bi Zhenzhen , Liu Yuhui , Liu Zhen , Zhang Junlian , Bai Jiangping 

TITLE=Expression of Potato StDRO1 in Arabidopsis Alters Root Architecture and Drought Tolerance

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.836063

DOI=10.3389/fpls.2022.836063

ISSN=1664-462X

ABSTRACT=<p>Potato (<italic>Solanum tuberosum</italic> L) is the third important crop for providing calories to a large human population, and is considered sensitive to moderately sensitive to drought stress conditions. The development of drought-tolerant, elite varieties of potato is a challenging task, which can be achieved through molecular breeding. Recently, the <italic>DEEPER ROOTING 1</italic> (<italic>DRO1</italic>) gene has been identified in rice, which influences plant root system and regulates grain yield under drought stress conditions. The potato StDRO1 protein is mainly localized in the plasma membrane of tobacco leaf cells, and overexpression analysis of <italic>StDRO1</italic> in Arabidopsis resulted in an increased lateral root number, but decreased lateral root angle, lateral branch angle, and silique angle. Additionally, the drought treatment analysis indicated that <italic>StDRO1</italic> regulated drought tolerance and rescued the defective root architecture and drought-tolerant phenotypes of <italic>Atdro1</italic>, an Arabidopsis <italic>AtDRO1</italic> null mutant. Furthermore, <italic>StDRO1</italic> expression was significantly higher in the drought-tolerant potato cultivar “Unica” compared to the drought-sensitive cultivar “Atlantic.” The transcriptional response of <italic>StDRO1</italic> under drought stress occurred significantly earlier in Unica than in Atlantic. Collectively, the outcome of the present investigation elucidated the role of DRO1 function in the alternation of root architecture, which potentially acts as a key gene in the development of a drought stress-tolerant cultivar. Furthermore, these findings will provide the theoretical basis for molecular breeding of drought-tolerant potato cultivars for the farming community.</p>